Cytokines and cell adhesion molecules associated with high-intensity eccentric exercise

  • L. L. Smith
  • A. Anwar
  • M. Fragen
  • C. Rananto
  • R. Johnson
  • D. Holbert
ORIGINAL ARTICLE

DOI: 10.1007/s004210050652

Cite this article as:
Smith, L., Anwar, A., Fragen, M. et al. Eur J Appl Physiol (2000) 82: 61. doi:10.1007/s004210050652

Abstract

Unaccustomed, eccentrically biased exercise induces trauma to muscle and/or connective tissue. Tissue damage activates an acute inflammatory response. Inflammation requires the effective interaction of different physiological and biological systems. Much of this is coordinated by the de novo synthesis of families of protein molecules, cytokines. The purpose of the present paper was to determine changes in blood levels of various cytokines in response to exercise-induced muscle damage that was effected using high-intensity eccentric exercise. Six healthy, untrained, college-age male subjects were required to perform the eccentric phase of a bench press and a leg curl (4 sets, 12 repetitions/set) at an intensity equivalent to 100% of their previously determined one-repetition maximum. Samples of blood were drawn at the following times: before exercise and 1.5, 6, 12, 24, 48, 72, 96, 120, and 144 h after exercise. These samples were analyzed for interleukins (IL): IL-lβ, IL-6, and IL-10; tumor necrosis factor-α; colony stimulating factors (CSF): granulocyte-CSF, macrophage-CSF, and GM-CSF; for cell adhesion molecules (CAM): P- and E-selectin, and intercellular cell adhesion molecule (ICAM-1), and vascular cell adhesion molecule (VCAM-1). Results were analyzed using a repeated-measures analysis of variance (P=0.05). Compared to baseline values, IL-1β was reduced (P=0.03) at 6, 24, and 96–144 h after exercise; IL-6 was elevated (P=0.01) at 12, 24, and 72 h after exercise; IL-10 was elevated (P=0.009) between 72 and 144 h after exercise; M-CSF was elevated (P=0.005) at 12 and 48–144 h after exercise; and P-selectin was reduced (P=0.01) between 24 and 144 h after exercise. It is concluded that when high-intensity eccentric exercise is compared to strenuous endurance exercise, post-exercise changes in cytokines do occur, but they are generally of a smaller magnitude, and occur at a later time period after the termination of exercise.

Key words Interleukins Tumor necrosis factor Colony stimulating factors Cell adhesion molecules 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • L. L. Smith
    • 1
  • A. Anwar
    • 2
  • M. Fragen
    • 2
  • C. Rananto
    • 3
  • R. Johnson
    • 3
  • D. Holbert
    • 4
  1. 1.Department of Exercise and Sport Science, 371 Ward Sports Medicine Building, East-Carolina University, Greenville, NC 27858, USA Tel.: +1-252-3284688; Fax: +1-252-3284689US
  2. 2.Center for Exercise Science and Cardiovascular Rehabilitation, Northeastern Illinois University, Chicago, IL 60625, USAUS
  3. 3.Department of Health, Leisure, and Exercise Science, Appalachian State University, Boone, NC 28608, USAUS
  4. 4.Department of Biostatistics, East Carolina University, Greenville, NC 27858, USAUS

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