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European Journal of Applied Physiology

, Volume 119, Issue 3, pp 633–643 | Cite as

Comparison between men and women of volume regulating hormones and aquaporin-2 excretion following graded central hypovolemia

  • Nandu Goswami
  • Johannes Reichmuth
  • Annarita Di Mise
  • Bianca Brix
  • Andreas Roessler
  • Mariangela Centrone
  • Marianna Ranieri
  • Annamaria Russo
  • Natale Gaspare De Santo
  • Grazia Tamma
  • Ferdinando Carlo SassoEmail author
  • Giovanna ValentiEmail author
Original Article

Abstract

Central hypovolemia induced by orthostatic loading causes reno-vascular changes that can lead to orthostatic intolerance. In this study, we investigated volume regulating hormonal responses and reno-vascular changes in male and female subjects as they underwent central hypovolemia, induced by graded lower body negative pressure (LBNP). Aquaporin-2 (AQP2) excretion was measured as a biomarker for the renal system response to vasopressin. 37 young healthy subjects (n = 19 males; n = 18 females) were subjected to graded LBNP until − 40 mmHg LBNP. Under resting conditions, males had significantly higher copeptin (a stable peptide derived from vasopressin) levels compared with females. Adrenocorticotropin (ACTH), adrenomedullin (ADM), vasopressin (AVP) and brain natriuretic peptide (BNP) were not affected by our experimental protocol. Nevertheless, an analysis of ADM and BNP with the data normalized as percentages of the baseline value data showed an increase from baseline to 10 min after recovery in the males in ADM and in the females in BNP. Analysis of BNP and ADM raises the possibility of a preferential adaptive vascular response to central hypovolemia in males as shown by the normalized increase in ADM, whereas females showed a preferential renal response as shown by the normalized increase in BNP. Furthermore, our results suggest that there might be a difference between men and women in the copeptin response to alterations in orthostatic loading, simulated either using LBNP or during posture changes.

Keywords

Orthostatic loading Lower body negative pressure Vasopressin Copeptin Adrenomedullin Aquaporins 

Abbreviations

ACTH

Adrenocorticotropic hormone

ADM

Adrenomedullin

ANP

Atrial natriuretic peptide

AQP2

Aquaporin 2

AVP

Arginine vasopressin

BNP

Brain natriuretic peptide

CVP

Central venous pressure

HUT

Head up tilt

LBNP

Lower body negative pressure

RAAS

Renin–angiotensin–aldosterone system

Notes

Acknowledgements

We thank the participants for their time and co-operation.

Author contributions

NG designed the experiments wrote the manuscript. JR designed the experiments, performed the experiments, and analyzed the data. ADM performed the experiments and analyzed the data. BB performed the experiments and analyzed the data. AR performed the experiments and analyzed the data. MC performed the experiments and analyzed the data. MR performed the experiments and analyzed the data. AR performed the experiments and analyzed the data. NGDS critical reading of the manuscript. GT performed the experiments and analyzed the data. FCS designed the experiments and wrote the manuscript. GV designed the experiments and wrote the manuscript.

Funding

This study was supported by ASI (Italian Space Agency, Grant number 2013-091-R.0) to FCS and GV.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Nandu Goswami
    • 1
  • Johannes Reichmuth
    • 1
  • Annarita Di Mise
    • 2
  • Bianca Brix
    • 1
  • Andreas Roessler
    • 1
  • Mariangela Centrone
    • 2
  • Marianna Ranieri
    • 2
  • Annamaria Russo
    • 2
  • Natale Gaspare De Santo
    • 3
  • Grazia Tamma
    • 2
  • Ferdinando Carlo Sasso
    • 3
    Email author
  • Giovanna Valenti
    • 2
    Email author
  1. 1.Physiology Division, Otto Loewi Research Center for Vascular Biology, Immunology and InflammationMedical University of GrazGrazAustria
  2. 2.Department of Biosciences, Biotechnologies and BiopharmaceuticsUniversity of Bari Aldo MoroBariItaly
  3. 3.Department of Advanced Medical and Surgical SciencesUniversità della Campania “L. Vanvitelli”CasertaItaly

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