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European Journal of Applied Physiology

, Volume 118, Issue 8, pp 1725–1736 | Cite as

Effects of high loading by eccentric triceps surae training on Achilles tendon properties in humans

  • Jeam Marcel Geremia
  • Bruno Manfredini Baroni
  • Maarten Frank Bobbert
  • Rodrigo Rico Bini
  • Fabio Juner Lanferdini
  • Marco Aurélio Vaz
Original Article

Abstract

Purpose

To document the magnitude and time course of human Achilles tendon adaptations (i.e. changes in tendon morphological and mechanical properties) during a 12-week high-load plantar flexion training program.

Methods

Ultrasound was used to determine Achilles tendon cross-sectional area (CSA), length and elongation as a function of plantar flexion torque during voluntary plantar flexion. Tendon force–elongation and stress–strain relationships were determined before the start of training (pre-training) and after 4 (post-4), 8 (post-8) and 12 (post-12) training weeks.

Results

At the end of the training program, maximum isometric force had increased by 49% and tendon CSA by 17%, but tendon length, maximal tendon elongation and maximal strain were unchanged. Hence, tendon stiffness had increased by 82%, and so had Young’s modulus, by 86%. Significant changes were first detected at post-4 in stiffness (51% increase) and Young’s modulus (87% increase), and at post-8 in CSA (15% increase).

Conclusions

Achilles tendon material properties already improved after 4 weeks of high-load training: stiffness increased while CSA remained unchanged. Tendon hypertrophy (increased CSA) was observed after 8 training weeks and contributed to a further increase in Achilles tendon stiffness, but tendon stiffness increases were mostly caused by adaptations in tissue properties.

Keywords

Human tendon stiffness Myotendinous junction Ultrasound Eccentric training 

Abbreviations

ANOVA

Analysis of variance

Baseline

Evaluation before control period

CSA

Cross-sectional area

EMG

Surface electromyography

ICC

Intraclass correlation coefficient

MTJ

Myotendinous junction

MVIC

Maximal voluntary isometric contraction

Post-12

After 12 high loading training weeks

Post-4

After 4 high loading training weeks

Post-8

After 8 high loading training weeks

Pre-training

After a 4-week control period

StiffnessABS

Slope of the force–elongation curve obtained from 50 to 100% of peak force of the weakest participant

StiffnessREL

Slope of the force–elongation curve obtained from 50 to 100% of maximal isometric voluntary force

TL

Tendon length

US

Ultrasound

W

Watts

YM

Young’s Modulus

YMABS

Slope of the stress–strain curve obtained from 50 to 100% of peak force of the weakest participant

YMREL

Slope of the stress–strain curve obtained from 50 to 100% of maximal isometric voluntary stress

Notes

Acknowledgements

The authors would like to acknowledge Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Programa Ciências Sem Fronteiras (CSF) and Financiadora de Estudos e Projetos (FINEP) from Brazil for financial support, and Amanda de Lima and Mayra Casa Nova for technical support.

Author contributions

Study conception and design: JMG, MAV, BMB, data acquisition: JMG, FJL. Analysis and interpretation of data: JMG, RRB, FJL, BMB, MFB, MAV. Drafting of manuscript: JMG, BMB, RRB, FJL, MFB, MAV. Critical revision: JMG, BMB, RRB, FJL, MFB, MAV.

Compliance with ethical standards

Conflict of interest

No conflicts of interest, financial or otherwise, are declared by the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratório de Biomecânica (LABIOMEC), Centro de Educação Física e DesportosUniversidade Federal de Santa Maria (UFSM)Santa MariaBrazil
  2. 2.Laboratório de Pesquisa do Exercício, Escola de Educação Física, Fisioterapia e DançaUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  3. 3.Departamento de FisioterapiaUniversidade Federal de Ciências da Saúde de Porto AlegrePorto AlegreBrazil
  4. 4.Department of Human Movement Sciences, Faculty of Behavioral and Movement SciencesVrije Universiteit Amsterdam, Amsterdam Movement SciencesAmsterdamThe Netherlands
  5. 5.La Trobe Rural Health SchoolLa Trobe UniversityBendigoAustralia

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