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European Journal of Applied Physiology

, Volume 116, Issue 4, pp 841–850 | Cite as

Effect of acute hypoxia on inspiratory muscle oxygenation during incremental inspiratory loading in healthy adults

  • Nada BasoudanEmail author
  • Babak Shadgan
  • Jordan A. Guenette
  • Jeremy Road
  • W. Darlene Reid
Original Article

Abstract

Purpose

To non-invasively examine the effect of acute hypoxia and inspiratory threshold loading (ITL) on inspiratory muscles [sternocleidomastoid (SCM), scalene (SA) and parasternal (PS)] oxygenation in healthy adults using near-infrared spectroscopy (NIRS).

Methods

Twenty healthy adults (12 M/8 F) were randomly assigned to perform two ITL tests while breathing a normoxic or hypoxic (FIO2 = 15 %) gas mixture. NIRS devices were placed over the SCM, PS, SA, and a control muscle, tibialis anterior (TA), to monitor oxygenated (O2Hb), deoxygenated (HHb), total hemoglobin (tHb) and tissue saturation index (TSI). With the nose occluded, subjects breathed normally for 4 min through a mouthpiece that was connected to a weighted threshold loading device. ITL began by adding a 100-g weight to the ITL device. Then, every 2 min 50-g was added until task failure. Vital signs, ECG and ventilatory measures were monitored throughout the protocol.

Result

Participants were 31 ± 12 year and had normal spirometry. At task failure, the maximum load and ventilatory parameters did not differ between the hypoxic and normoxic ITL. At hypoxic ITL task failure, SpO2 was significantly lower, and ∆HHb increased more so in SA, SCM and PS than normoxic values. SCM ∆TSI decreased more so during hypoxic compared to normoxic ITL. ∆tHb in the inspiratory muscles (SCM, PS and SA) increased significantly compared to the decrease in TA during both hypoxic and normoxic ITL.

Conclusion

The SCM, an accessory inspiratory muscle was the most vulnerable to deoxygenation during incremental loading and this response was accentuated by acute hypoxia.

Keywords

Inspiratory muscle Respiratory muscle Near-infrared spectroscopy NIRS Acute hypoxia Inspiratory load 

Abbreviations

FEV1

Forced expiratory volume in 1 s

FEV1/FVC ratio

Ratio of the forced expiratory volume in the first 1 s to the forced vital capacity of the lungs

FIO2

Fraction of inspired oxygen

FVC

Forced vital capacity

HHb

Deoxygenated hemoglobin

ITL

Inspiratory threshold loading

NIRS

Near-infrared spectroscopy

O2Hb

Oxygenated hemoglobin

PETCO2

End tidal carbon dioxide partial pressure

PmPeak

Peak mouth pressure

PS

Parasternal

SA

Scalene

SCM

Sternocleidomastoid

SpO2

Saturation of hemoglobin with oxygen as measured by pulse oximetry

TA

Tibialis anterior

tHb

Total hemoglobin

TSI

Tissue saturation index

VE

Minute ventilation

VT

Tidal volume

Notes

Acknowledgments

We thank all subjects for their participation. We are also very appreciative of the valuable assistance given by Carmen Ma during data collection and Dr. Salem Bahubeshi for ECG monitoring. This study was supported by a grant and scholarship funding from Princess Nora bint Abdul Rahman University.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Department of Physical TherapyUniversity of British Columbia (UBC)VancouverCanada
  2. 2.College of Health and Rehabilitation SciencesPrincess Nora Bint Abdul Rahman UniversityRiyadhSaudi Arabia
  3. 3.International Collaboration on Repair DiscoveriesVancouverCanada
  4. 4.Centre for Heart Lung InnovationUBC and St. Paul’s HospitalVancouverCanada
  5. 5.Division of Respiratory Medicine, Department of MedicineUBCVancouverCanada
  6. 6.Physical Therapy DepartmentUniversity of TorontoTorontoCanada

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