European Journal of Applied Physiology

, Volume 114, Issue 4, pp 805–814 | Cite as

Energy expenditure and substrate oxidation during and after eccentric cycling

  • Luis Peñailillo
  • Anthony Blazevich
  • Kazunori Nosaka
Original Article

Abstract

Purpose

This study compared concentric cycling (CONC) and two bouts of eccentric cycling (ECC1, ECC2) for substrate utilisation and resting energy expenditure (REE).

Methods

Ten men (28 ± 8 years) performed each cycling bout for 30 min at 60 % of maximal concentric power output, with 2 weeks between bouts. Fat and carbohydrate (CHO) utilisation were assessed during and after cycling, and REE was measured before and 2 days after CONC, and before, 2 and 4 days after ECC1 and ECC2, using indirect calorimetry. An oral glucose tolerance test was performed before and 1 day after CONC, and before and 1 and 3 days after ECC1 and ECC2, and both peak and area-under-the-curve (AUC) of the glucose concentration were compared between bouts.

Results

Energy expenditure and CHO utilisation during cycling were 36 and 42 % less in ECC1, and 40 and 52 % less in ECC2, than CONC (P < 0.05). Fat utilisation was greater during ECC1 (72 %) and ECC2 (85 %) than CONC, and 48 % greater during ECC2 than ECC1 (P < 0.05). Post-exercise energy expenditure and fat utilisation were less for ECC1 than CONC (30 and 52 %, respectively), but similar between CONC and ECC2. REE did not change from baseline after any bouts. Peak and AUC glucose concentration decreased 3 days after ECC1, but no changes were evident after CONC or ECC2.

Conclusion

These results show greater fat utilisation during eccentric than concentric cycling at the same workload, and greater fat oxidation during and after secondary eccentric cycling bout without glucose uptake impairment.

Keywords

Fat oxidation Carbohydrate oxidation Glucose tolerance Recumbent bicycle Repeated bout effect Muscle damage 

Abbreviations

CHO

Carbohydrates

VCO2/VO2

Carbon dioxide production/oxygen consumption

RER

Respiratory exchange ratio

REE

Resting energy expenditure

BMI

Body mass index

OGTT

Oral glucose tolerance test

CONC

Concentric cycling

ECC1

First eccentric cycling bout

ECC1

Second eccentric cycling bout

POmax

Maximal power output

VO2peak

Peak oxygen consumption

VO2max

Maximal oxygen consumption

AUC

Area-under-the-curve

CV

Coefficient of variation

ANOVA

Analysis of variance

CK activity

Creatine kinase activity

RM

Repetition maximum

References

  1. Abbott BC, Bigland B, Ritchie JM (1952) The physiological cost of negative work. J Physiol 117(3):380–390PubMedCentralPubMedGoogle Scholar
  2. Allen DG (2001) Eccentric muscle damage: mechanisms of early reduction of force. Acta Physiol Scand 171(3):311–319PubMedCrossRefGoogle Scholar
  3. Asp S, Daugaard JR, Richter EA (1995) Eccentric exercise decreases glucose transporter GLUT4 protein in human skeletal muscle. J Physiol 482(3):705–712PubMedCentralPubMedGoogle Scholar
  4. Binzen CA, Swan PD, Manore MM (2001) Postexercise oxygen consumption and substrate use after resistance exercise in women. Med Sci Sports Exerc 33(6):932–938PubMedCrossRefGoogle Scholar
  5. Compher C, Frankenfield D, Keim N, Roth-Yousey L (2006) Best practice methods to apply to measurement of resting metabolic rate in adults: a systematic review. J Am Diet Assoc 106(6):881–903PubMedCrossRefGoogle Scholar
  6. Cooper JA, Watras AC, O’Brien MJ, Luke A, Dobratz JR, Earthman CP, Schoeller DA (2009) Assessing validity and reliability of resting metabolic rate in six gas analysis systems. J Am Diet Assoc 109(1):128–132PubMedCentralPubMedCrossRefGoogle Scholar
  7. De Pauw K, Roelands B, Cheung SS, de Geus B, Rietjens G, Meeusen R (2013) Guidelines to classify subject groups in sport-science research. Int J Sports Physiol Perform 8(2):111–122PubMedGoogle Scholar
  8. Dolezal BA, Potteiger JA (1998) Concurrent resistance and endurance training influence basal metabolic rate in nondieting individuals. J Appl Physiol 85(2):695–700PubMedGoogle Scholar
  9. Dolezal BA, Potteiger JA, Jacobsen DJ, Benedict SH (2000) Muscle damage and resting metabolic rate after acute resistance exercise with an eccentric overload. Med Sci Sports Exerc 32(7):1202–1207PubMedCrossRefGoogle Scholar
  10. Drexel H, Saely CH, Langer P, Loruenser G, Marte T, Risch L, Hoefle G, Aczel S (2008) Metabolic and anti-inflammatory benefits of eccentric endurance exercise—a pilot study. Eur J Clin Invest 38(4):218–226PubMedCrossRefGoogle Scholar
  11. Dumortier M, Thoni G, Brun JF, Mercier J (2005) Substrate oxidation during exercise: impact of time interval from the last meal in obese women. Int J Obes 29(8):966–974CrossRefGoogle Scholar
  12. Fabbri LM, Luppi F, Beghe B, Rabe KF (2008) Complex chronic comorbidities of COPD. Eur Res J 31(1):204–212CrossRefGoogle Scholar
  13. Fielding RA, Meredith CN, O’Reilly KP, Frontera WR, Cannon JG, Evans WJ (1991) Enhanced protein breakdown after eccentric exercise in young and older men. J Appl Physiol 71(2):674–679PubMedGoogle Scholar
  14. Green MS, Doyle J, Ingalls C, Benardot D, Rupp J, Corona B (2010) Adaptation of insulin-resistance indicators to a repeated bout of eccentric exercise in human skeletal muscle. Int J Sport Nut Exerc Met 20:181–190Google Scholar
  15. Haskell WL, Lee IM, Pate RR, Powell KE, Blair SN, Franklin BA, Macera CA, Heath GW, Thompson PD, Bauman A (2007) Physical activity and public health: updated recommendation for adults from the American College of Sports Medicine and the American Heart Association. Med Sci Sports Exerc 39(8):1423–1434PubMedCrossRefGoogle Scholar
  16. Heden T, Lox C, Rose P, Reid S, Kirk EP (2011) One-set resistance training elevates energy expenditure for 72 h similar to three sets. Eur J Appl Physiol 111(3):477–484PubMedCentralPubMedCrossRefGoogle Scholar
  17. Hody S, Leprince P, Sergeant K, Renaut J, Croisier JL, Wang F, Rogister B (2011) Human muscle proteome modifications after acute or repeated eccentric exercises. Med Sci Sports Exerc 43(12):2281–2296PubMedCrossRefGoogle Scholar
  18. Jamurtas AZ, Koutedakis Y, Paschalis V, Tofas T, Yfanti C, Tsiokanos A, Koukoulis G, Kouretas D, Loupos D (2004) The effects of a single bout of exercise on resting energy expenditure and respiratory exchange ratio. Eur J Appl Physiol 92(4–5):393–398PubMedGoogle Scholar
  19. Jeukendrup AE, Wallis GA (2005) Measurement of substrate oxidation during exercise by means of gas exchange measurements. Int J Sports Med 26(Suppl 1):S28–S37PubMedCrossRefGoogle Scholar
  20. Jeukendrup AE, Saris WH, Wagenmakers AJ (1998) Fat metabolism during exercise: a review—part II: regulation of metabolism and the effects of training. Int J Sports Med 19(5):293–302PubMedCrossRefGoogle Scholar
  21. Kirwan JP, Hickner RC, Yarasheski KE, Kohrt WM, Wiethop BV, Holloszy JO (1992) Eccentric exercise induces transient insulin resistance in healthy individuals. J Appl Physiol 72(6):2197–2202PubMedCrossRefGoogle Scholar
  22. Knuttgen HG, Patton JF, Vogel JA (1982) An ergometer for concentric and eccentric muscular exercise. J Appl Physiol 53(3):784–788PubMedGoogle Scholar
  23. Krishnan RK, Evans WJ, Kirwan JP (2003) Impaired substrate oxidation in healthy elderly men after eccentric exercise. J Appl Physiol 94(2):716–723PubMedGoogle Scholar
  24. LaStayo PC, Pierotti DJ, Pifer J, Hoppeler H, Lindstedt SL (2000) Eccentric ergometry: increases in locomotor muscle size and strength at low training intensities. Am J Physiol Regul Integr Comp Physiol 278(5):R1282–R1288PubMedGoogle Scholar
  25. LaStayo PC, Ewy GA, Pierotti DD, Johns RK, Lindstedt S (2003) The positive effects of negative work: increased muscle strength and decreased fall risk in a frail elderly population. J Gerontol A Biol Sci Med Sci 58(5):M419–M424PubMedCrossRefGoogle Scholar
  26. Lazzer S, Lafortuna C, Busti C, Galli R, Tinozzi T, Agosti F, Sartorio A (2010) Fat oxidation rate during and after a low- or high-intensity exercise in severely obese Caucasian adolescents. Eur J Appl Physiol 108(2):383–391PubMedCrossRefGoogle Scholar
  27. Marcus RL, Lastayo PC, Dibble LE, Hill L, McClain DA (2009) Increased strength and physical performance with eccentric training in women with impaired glucose tolerance: a pilot study. J Womens Health 18(2):253–260CrossRefGoogle Scholar
  28. McHugh MP (2003) Recent advances in the understanding of the repeated bout effect: the protective effect against muscle damage from a single bout of eccentric exercise. Scand J Med Sci Sports 13(2):88–97PubMedCrossRefGoogle Scholar
  29. Nordby P, Saltin B, Helge JW (2006) Whole-body fat oxidation determined by graded exercise and indirect calorimetry: a role for muscle oxidative capacity? Scand J Med Sci Sports 16(3):209–214PubMedCrossRefGoogle Scholar
  30. Nosaka K, Clarkson PM (1995) Muscle damage following repeated bouts of high force eccentric exercise. Med Sci Sports Exerc 27(9):1263–1269PubMedCrossRefGoogle Scholar
  31. Panayiotou G, Paschalis V, Nikolaidis MG, Theodorou AA, Deli CK, Fotopoulou N, Fatouros IG, Koutedakis Y, Sampanis M, Jamurtas AZ (2013) No adverse effects of statins on muscle function and health-related parameters in the elderly: an exercise study. Scand J Med Sci Sports 23(5):556–567PubMedGoogle Scholar
  32. Paschalis V, Nikolaidis MG, Giakas G, Theodorou AA, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ (2010a) Beneficial changes in energy expenditure and lipid profile after eccentric exercise in overweight and lean women. Scand J Med Sci Sports 20(1):e103–e111PubMedCrossRefGoogle Scholar
  33. Paschalis V, Nikolaidis MG, Theodorou AA, Panayiotou G, Fatouros IG, Koutedakis Y, Jamurtas AZ (2010b) A weekly bout of eccentric exercise is sufficient to induce health-promoting effects. Med Sci Sports Exerc 43(1):64–73CrossRefGoogle Scholar
  34. Penailillo L, Blazevich A, Numazawa H, Nosaka K (2013) Metabolic and muscle damage profiles of concentric versus repeated eccentric cycling. Med Sci Sports Exerc 45(9):1773–1781PubMedCrossRefGoogle Scholar
  35. Perrey S, Betik A, Candau R, Rouillon JD, Hughson RL (2001) Comparison of oxygen uptake kinetics during concentric and eccentric cycle exercise. J Appl Physiol 91(5):2135–2142PubMedGoogle Scholar
  36. Phelain JF, Reinke E, Harris MA, Melby CL (1997) Postexercise energy expenditure and substrate oxidation in young women resulting from exercise bouts of different intensity. J Am Coll Nutr 16(2):140–146PubMedCrossRefGoogle Scholar
  37. Rumpler WV, Seale JL, Conway JM, Moe PW (1990) Repeatability of 24-h energy expenditure measurements in humans by indirect calorimetry. Am J Clin Nutr 51(2):147–152PubMedGoogle Scholar
  38. Schuenke MD, Mikat RP, McBride JM (2002) Effect of an acute period of resistance exercise on excess post-exercise oxygen consumption: implications for body mass management. Eur J Appl Physiol 86(5):411–417PubMedCrossRefGoogle Scholar
  39. Sedlock DA (1992) A comparison of postexercise energy-expenditure following treadmill and cycle ergometry. Res Q Exerc Sport 63(1):A28Google Scholar
  40. Segal KR (1987) Comparison of indirect calorimetric measurements of resting energy expenditure with a ventilated hood, face mask, and mouthpiece. Am J Clin Nut 45(6):1420–1423Google Scholar
  41. Smith J, Mc Naughton L (1993) The effects of intensity of exercise on excess postexercise oxygen consumption and energy expenditure in moderately trained men and women. Eur J Appl Physiol Occup Physiol 67(5):420–425PubMedCrossRefGoogle Scholar
  42. Smith J, McNaughton L (1993) The effects of intensity of exercise on excess postexercise oxygen consumption and energy expenditure in moderately trained men and women. Eur J Appl Physiol Occup Physiol 67(5):420–425PubMedCrossRefGoogle Scholar
  43. Venables MC, Achten J, Jeukendrup AE (2005) Determinants of fat oxidation during exercise in healthy men and women: a cross-sectional study. J Appl Physiol 98(1):160–167PubMedCrossRefGoogle Scholar
  44. Walsh-Riddle M, Blumenthal JA (1989) Cardiovascular responses during upright and semi-recumbent cycle ergometry testing. Med Sci Sports Exerc 21(5):581–585PubMedCrossRefGoogle Scholar
  45. Weir JB (1949) New methods for calculating metabolic rate with special reference to protein metabolism. J Physiol 109(1–2):1–9PubMedCentralPubMedGoogle Scholar
  46. Zurlo F, Lillioja S, Esposito-Del Puente A, Nyomba BL, Raz I, Saad MF, Swinburn BA, Knowler WC, Bogardus C, Ravussin E (1990) Low ratio of fat to carbohydrate oxidation as predictor of weight gain: study of 24-h RQ. Am J Physiol 259(5 Pt 1):E650–E657PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Luis Peñailillo
    • 1
    • 2
  • Anthony Blazevich
    • 1
  • Kazunori Nosaka
    • 1
  1. 1.Centre for Exercise and Sports Science Research, School of Exercise and Health SciencesEdith Cowan UniversityJoondalupAustralia
  2. 2.Exercise Science Laboratory, Faculty of Medicine, School of KinesiologyUniversidad Finis TerraeProvidenciaChile

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