The effects of aerobic exercise training at two different intensities in obesity and type 2 diabetes: implications for oxidative stress, low-grade inflammation and nitric oxide production
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To investigate the effect of 16 weeks of aerobic training performed at two different intensities on nitric oxide (tNOx) availability and iNOS/nNOS expression, oxidative stress (OS) and inflammation in obese humans with or without type 2 diabetes mellitus (T2DM).
Twenty-five sedentary, obese (BMI > 30 kg/m2) males (52.8 ± 7.2 years); 12 controls versus 13 T2DM were randomly allocated to four groups that exercised for 30 min, three times per week either at low (Fat-Max; 30–40 % VO2max) or moderate (T vent; 55–65 % VO2max) intensity. Before and after training, blood and muscle samples (v. lateralis) were collected.
Baseline erythrocyte glutathione was lower (21.8 ± 2.8 vs. 32.7 ± 4.4 nmol/ml) and plasma protein oxidative damage and IL-6 were higher in T2DM (141.7 ± 52.1 vs. 75.5 ± 41.6 nmol/ml). Plasma catalase increased in T2DM after T vent training (from 0.98 ± 0.22 to 1.96 ± 0.3 nmol/min/ml). T2DM groups demonstrated evidence of oxidative damage in response to training (elevated protein carbonyls). Baseline serum tNOx were higher in controls than T2DM (18.68 ± 2.78 vs. 12.34 ± 3.56 μmol/l). Training at T vent increased muscle nNOS and tNOx in the control group only. Pre-training muscle nNOS was higher in controls than in T2DMs, while the opposite was found for iNOS. No differences were found after training for plasma inflammatory markers.
Exercise training did not change body composition or aerobic fitness, but improved OS markers, especially when performed at T vent. Non-diabetics responded to T vent training by increasing muscle nNOS expression and tNOx levels in skeletal muscle while these parameters did not change in T2DM, perhaps due to higher insulin resistance (unchanged after intervention).
KeywordsDiabetes Obesity Adipokines Oxidative stress Nitric oxide Blood Skeletal muscle
Nitric oxide metabolites
Type 2 diabetes mellitus
This work was supported by the Institute of Technology Technological Sector Research (TSR): Strand III—Core Research Strengths Enhancement (Ireland) and the Irish Research Council for Science, Engineering and Technology.
Conflict of interest
The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.
- Fayh AP, Krause M, Rodrigues-Krause J, Ribeiro JL, Ribeiro JP, Friedman R, Moreira JC, Reischak-Oliveira A (2013) Effects of L-arginine supplementation on blood flow, oxidative stress status and exercise responses in young adults with uncomplicated type I diabetes. Eur J Nutr 52(3):975–983Google Scholar
- Krause MS, Oliveira LP Jr, Silveira EM, Vianna DR, Rossato JS, Almeida BS, Rodrigues MF, Fernandes AJ, Costa JA, Curi R, de Bittencourt PI Jr (2007) MRP1/GS-X pump ATPase expression: is this the explanation for the cytoprotection of the heart against oxidative stress-induced redox imbalance in comparison to skeletal muscle cells? Cell Biochem Funct 25:23–32PubMedCrossRefGoogle Scholar
- Krause M, Bittencourt A, Homem de Bittencourt PI, McClenaghan NH, Flatt PR, Murphy C, Newsholme P (2012a) Physiological concentrations of interleukin-6 directly promote insulin secretion, signal transduction, nitric oxide release, and redox status in a clonal pancreatic β-cell line and mouse islets. J Endocrinol 214(3):301–311Google Scholar
- Krause M, Rodrigues-Krause J, O’Hagan C, De Vito G, Boreham C, Susta D, Newsholme P, Murphy C (2012b) Differential nitric oxide levels in the blood and skeletal muscle of type 2 diabetic subjects may be consequence of adiposity: a preliminary study. Metabolism 61(11):1528–1537Google Scholar
- Madsen EL, Rissanen A, Bruun JM, Skogstrand K, Tonstad S, Hougaard DM, Richelsen B (2008) Weight loss larger than 10 % is needed for general improvement of levels of circulating adiponectin and markers of inflammation in obese subjects: a 3-year weight loss study. Eur J Endocrinol 158:179–187PubMedCrossRefGoogle Scholar
- Monti LD, Setola E, Lucotti PC, Marrocco-Trischitta MM, Comola M, Galluccio E, Poggi A, Mammi S, Catapano AL, Comi G, Chiesa R, Bosi E, Piatti PM (2012) Effect of a long-term oral l-arginine supplementation on glucose metabolism: a randomized, double-blind, placebo-controlled trial. Diabetes Obes Metab 14(10):893–900Google Scholar
- Thomas DE, Elliott EJ, Naughton GA (2006) Exercise for type 2 diabetes mellitus. Cochrane Database Syst Rev CD002968Google Scholar
- Wisse W, Rookhuizen MB, de Kruif MD, van Rossum J, Jordans I, ten Cate H, van Loon LJ, Meesters EW (2010) Prescription of physical activity is not sufficient to change sedentary behavior and improve glycemic control in type 2 diabetes patients. Diabetes Res Clin Pract 88:e10–e13PubMedCrossRefGoogle Scholar