European Journal of Applied Physiology

, Volume 113, Issue 10, pp 2613–2619 | Cite as

Influence of CMV/EBV serostatus on respiratory infection incidence during 4 months of winter training in a student cohort of endurance athletes

  • Cheng-Shiun He
  • Michal Handzlik
  • Ayu Muhamad
  • Michael GleesonEmail author
Original Article



The purpose of this study was to examine the influence of previous infection with cytomegalovirus (CMV) or Epstein Barr virus (EBV) on the incidence, severity and duration of upper respiratory tract infections (URTIs) in endurance athletes during a 4-month winter training period.


Blood samples were obtained from 236 subjects (166 males and 70 females, aged 18–35 years) at the start of the study period. Plasma samples were analysed for CMV and EBV serostatus. Weekly training and daily illness logs were kept for the next 16 weeks.


With regard to CMV/EBV serostatus, the results indicated that athletes with previous CMV infection (n = 58, 25 % of cohort) had significantly fewer URTI symptom days (median 2 vs. 4 days, p = 0.033) during the study period than those with no previous infection (n = 178, 75 %), whereas positive EBV serostatus (n = 197, 84 %) had no influence on URTI episode incidence, severity or duration. Moreover, we found that athletes with prior infection of both CMV and EBV (n = 50, 21 %) had 50 % fewer URTI episodes (p = 0.04) and symptom days (median 2 vs 8 days, p = 0.01) than athletes who were seronegative for both CMV and EBV (n = 31, 13 %).


Previous coinfection with CMV and EBV might promote protective immune surveillance to lower the risk of URTI. Further research is required to clarify why previous CMV and EBV infection reduces the incidence of URTI.


Exercise training Herpesvirus T lymphocytes Common cold 



This study was supported by a grant from the Gatorade Sports Science Institute.


  1. Bate SL, Dollard SC, Cannon MJ (2010) Cytomegalovirus seroprevalence in the United States: the national health and nutrition examination surveys, 1988–2004. Clin Infect Dis 50(11):1439–1447PubMedCrossRefGoogle Scholar
  2. Chang WL, Barry PA (2010) Attenuation of innate immunity by cytomegalovirus IL-10 establishes a long-term deficit of adaptive anti-viral immunity. PNAS 107:22647–22652PubMedCrossRefGoogle Scholar
  3. Craig CL, Marshall AL, Sjostrom M, Bauman AE, Booth ML, Ainsworth BE, Pratt M, Ekelund U, Yngve A, Sallis JF, Oja P (2003) International physical activity questionnaire: 12-country reliability and validity. Med Sci Sports Exerc 35(8):1381–1395PubMedCrossRefGoogle Scholar
  4. Fahlman MM, Engels HJ (2005) Mucosal IgA and URTI in American college football players: a year longitudinal study. Med Sci Sports Exerc 37(3):374–380PubMedCrossRefGoogle Scholar
  5. Gleeson M, Walsh NP (2012) The BASES expert statement on exercise, immunity, and infection. J Sports Sci 30(3):321–324PubMedCrossRefGoogle Scholar
  6. Gleeson M, Pyne DB, Austin JP, Lynn Francis J, Clancy RL, McDonald WA (2002) Epstein–Barr virus reactivation and upper-respiratory illness in elite swimmers. Med Sci Sports Exerc 34(3):411–417PubMedCrossRefGoogle Scholar
  7. Gleeson M, Bishop N, Oliveira M, McCauley T, Tauler P, Muhamad AS (2012) Respiratory infection risk in athletes: association with antigen-stimulated IL 10 production and salivary IgA secretion. Scand J Med Sci Sports 22:410–417PubMedCrossRefGoogle Scholar
  8. Gleeson M, Bishop N, Oliveira M, McCauley T, Tauler P (2013) Influence of training load on upper respiratory tract infection incidence and antigen-stimulated cytokine production in whole blood culture. Scand J Med Sci Sports 23:451–457PubMedCrossRefGoogle Scholar
  9. He CS, Handzlik M, Fraser WD, Muhamad A, Preston H, Richardson A, Gleeson M (2013) Influence of vitamin D status on respiratory infection incidence and immune function during 4 months of winter training in endurance sport athletes. Exerc Immunol Rev 19:86–101PubMedGoogle Scholar
  10. Jackson GG, Dowling HF, Spiesman IG, Boand AV (1958) Transmission of the common cold to volunteers under controlled conditions: the common cold as a clinical entity. Arch Intern Med 101:267–278CrossRefGoogle Scholar
  11. Liu Y, de Waal Malefyt R, Briere F, Parham C, Bridon JM, Banchereau J, Moore KW, Xu J (1997) The EBV IL-10 homologue is a selective agonist with impaired binding to the IL-10 receptor. J Immunol 158(2):604–613PubMedGoogle Scholar
  12. Niederman JC, Evans AS, Subrahmanyan L, McCollum RW (1970) Prevalence, incidence and persistence of EB virus antibody in young adults. N Engl J Med 282:361–365PubMedCrossRefGoogle Scholar
  13. Nieman DC (1994) Exercise, infection, and immunity. Int J Sports Med 15(Suppl 3):S131–S141PubMedCrossRefGoogle Scholar
  14. Predy GN, Goel V, Lovlin R, Donner A, Stitt L, Basu TK (2005) Efficacy of an extract of North American ginseng containing poly-furanosyl-pyranosyl-saccharides for preventing upper respiratory tract infections: a randomized controlled trial. Can Med Assoc J 173(9):1043–1048CrossRefGoogle Scholar
  15. Peters EM, Goetzsche JM, Grobbelaar B, Noakes TD (1993) Vitamin C supplementation reduces the incidence of postrace symptoms of upper-respiratory-tract infection in ultramarathon runners. Am J Clin Nutr 57(2):170–174PubMedGoogle Scholar
  16. Pottgiesser T, Wolfarth B, Schumacher YO, Bauer G (2006) Epstein–Barr virus serostatus: no difference despite aberrant patterns in athletes and control group. Med Sci Sports Exerc 38(10):1782–1791PubMedCrossRefGoogle Scholar
  17. Turner JE, Aldred S, Witard OC, Drayson MT, Moss PM, Bosch JA (2010) Latent cytomegalovirus infection amplifies CD8 T-lymphocyte mobilisation and egress in response to exercise. Brain Behav Immun 24(8):1362–1370PubMedCrossRefGoogle Scholar
  18. Wherry EJ, Ahmed R (2004) Memory CD8 T-cell differentiation during viral infection. J Virol 78(11):5535–5545PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Cheng-Shiun He
    • 1
  • Michal Handzlik
    • 2
  • Ayu Muhamad
    • 1
  • Michael Gleeson
    • 1
    Email author
  1. 1.School of Sport, Exercise and Health SciencesLoughborough UniversityLeicestershireUK
  2. 2.School of Biomedical SciencesNottingham UniversityNottinghamUK

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