Distinctive bronchial inflammation status in athletes: basophils, a new player
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The aim of the study was to establish bronchial inflammation status and to measure eicosanoids in sputum obtained from active elite athletes. A total of 68 subjects were enrolled. Twelve were non-athletes and non-asthmatic (NAtNAs), 21 non-athlete asthmatics (NAtAs), 11 athlete non-asthmatics (AtNAs), and 24 athletes with asthma (AtAs) with positive indirect or direct bronchial challenges. Induced sputum was used to measure cells and eicosanoids. Sputum differential cell counts in all the subject groups revealed eosinophilia with the exception of NAtNAs control subjects. Athletes with and without diagnosed asthma showed a significant increase in bronchial epithelial cells and lymphocytes present in their sputum. Also, flow cytometry revealed that a significantly higher number of basophils were present in sputum from athletes (without and with asthma) when compared with non-athletes (without and with asthma). Asthmatic athletes and non-athletes showed a higher increase in LTC4 levels and PGE2 metabolites in sputum when compared with healthy controls. The present study identifies basophils as a new player present in athletes bronchial inflammation defining athlete status and not necessarily associated with exercise-induced bronchoconstriction.
KeywordsAsthma Cellular inflammation Basophils Epithelial cells Eicosanoid mediators
This study was supported by Universidad Europea de Madrid (Cátedra Real Madrid) and CIBER de Enfermedades Respiratorias (CIBERES), an initiative of Carlos III Institute of Health. The authors recognize Oliver Shaw for his revision and editing in English and Ignacio Mahillo for statistical support.
- American Thoracic Society. Standards for the diagnosis and care of patients with chronic obstructive pulmonary disease (COPD) and asthma (1987) This official statement of the American Thoracic Society was adopted by the ATS Boards of Directors, November 1986. Am Rev Respir Dis 136:225–244Google Scholar
- Carlsen KH, Anderson SD, Bjermer L et al (2008) European Respiratory Society; European Academy of Allergy and Clinical Immunology. Exercise-induced asthma, respiratory and allergic disorders in elite athletes: epidemiology, mechanisms and diagnosis: part I of the report from the Joint Task Force of the European Respiratory Society (ERS) and the European Academy of Allergy and Clinical Immunology (EAACI) in cooperation with GA2LEN. Allergy 63:387–403PubMedCrossRefGoogle Scholar
- Domínguez-Ortega J, León F, Martínez Alonso JC et al (2004) Fluorocytometric analysis of induced sputum cells in an asthmatic population. J Invest Allergol Clin Immunol 14:108–113Google Scholar
- Fahy JV (2001) Remodeling of the airway epithelium in asthma. Am J Respir Crit Care Med 164:546–551Google Scholar
- Global strategy for asthma management and prevention (2002) Update from NHLB/WHO Workshop Report GINA, revised 2002, NIH Publication No. 02-3659Google Scholar
- Hening NR, Aitken ML, Liu MC et al (2000) Effect of recombinant human platelet-activating factor-acetylhydrolase on allergen-induced asthmatic responses. Am J Respir Crit care Med 162:523–527Google Scholar