European Journal of Applied Physiology

, Volume 112, Issue 11, pp 3755–3764

Analysis of both pulsatile and streamline blood flow patterns during aerobic and resistance exercise

Original Article

Abstract

Blood flow-induced endothelial shear stress (ESS) during aerobic (AX) and resistance (RX) exercise can regulate endothelial function. However, non-invasive in vivo ESS estimation is normally obtained only according to Poiseuille’s laws for streamline flow, rather than using Womersley’s approximation for pulsatile flows. Here, we sought to determine brachial and femoral artery blood flow patterns, based on ESS, flow direction, and flow turbulence, using both pulsatile and streamline flow approximations during low- and moderate-intensity AX and RX. We performed high-resolution ultrasound imaging and Doppler peak blood flow velocity (V) measurements of the brachial and femoral arteries in eight young, healthy men during rest and two intensities of AX and RX at 40 and 70% of VO2max and 1-RM, respectively. Microhematocrit measurement was used to determine blood density (ρ) and viscosity (μ). ESS was calculated using Poiseuille’s law, ESS = 2μ × SR (V/artery diameter), and Womersley’s approximation, ESS = 2 Kμ × SR, where K is a function of Womersley’s parameter α. Turbulence was determined using Reynolds number (Re). Re was calculated using Re = V × artery diameter × ρ/μ and normalized to resting steady-state values (nRe). ESS increases in a dose-dependent manner in the femoral and brachial arteries during both AX and RX when using either streamline or pulsatile approximations. However, our findings indicate that ESS is underestimated when using Poiseuille’s law. Secondly, turbulence increases in conduit arteries with exercise intensity in a dose-dependent manner in both retrograde and antegrade flows during both AX and RX.

Keywords

Endothelial shear stress Poiseuille’s law Womersley’s approximation Turbulence Reynolds number 

References

  1. ACSM (2000) American College of Sports Medicine’s Guidelines for exercise testing and prescription. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
  2. Adkisson EJ, Casey Darren P, Beck Darren T, Gurovich Alvaro N, Martin Jeffery S, Braith Randy W (2010) Central, peripheral, and resistance arterial reactivity fluctuates during the phases of the menstrual cycle. Exp Biol Med 235:111–118CrossRefGoogle Scholar
  3. Braith RW, Conti CR, Nichols WW, Choi CY, Khuddus MA, Beck DT, Casey DP (2010) Enhanced external counterpulsation improves peripheral artery flow-mediated dilation in patients with chronic angina: a randomized sham-controlled study. Circulation 122:1612–1620PubMedCrossRefGoogle Scholar
  4. Charlesworth D (1981) Relationship of blood rheology to blood flow. In: Lowe G, Barbenel J, Forbes C (eds) Clinical aspects of blood viscosity and cell deformability. Springer, New York, pp 91–96CrossRefGoogle Scholar
  5. Chatzizisis YS, Coskun AU, Jonas M, Edelman ER, Feldman CL, Stone PH (2007) Role of endothelial shear stress in the natural history of coronary atherosclerosis and vascular remodeling: molecular, cellular, and vascular behavior. J Am Coll Cardiol 49:2379–2393PubMedCrossRefGoogle Scholar
  6. Cheng C, van Haperen R, de Waard M, van Damme LC, Tempel D, Hanemaaijer L, van Cappellen GW, Bos J, Slager CJ, Duncker DJ, van der Steen AF, de Crom R, Krams R (2005) Shear stress affects the intracellular distribution of eNOS: direct demonstration by a novel in vivo technique. Blood 106:3691–3698PubMedCrossRefGoogle Scholar
  7. Cheng C, Tempel D, van Haperen R, van der Baan A, Grosveld F, Daemen MJ, Krams R, de Crom R (2006) Atherosclerotic lesion size and vulnerability are determined by patterns of fluid shear stress. Circulation 113:2744–2753PubMedCrossRefGoogle Scholar
  8. Davies PF (2009) Hemodynamic shear stress and the endothelium in cardiovascular pathophysiology. Nat Clin Pract Cardiovasc Med 6:16–26PubMedCrossRefGoogle Scholar
  9. Davies PF, Remuzzi A, Gordon EJ, Dewey CF Jr, Gimbrone MA Jr (1986) Turbulent fluid shear stress induces vascular endothelial cell turnover in vitro. Proc Nat Acad Sci USA 83:2114–2117PubMedCrossRefGoogle Scholar
  10. Dormandy J (1981) Measurement of whole-blood viscosity. In: Lowe G, Barbenel J, Forbes C (eds) Clinical aspects of blood viscosity and cell deformability. Springer, New York, pp 67–78CrossRefGoogle Scholar
  11. Feldman AM (2002) Enhanced external counterpulsation: mechanism of action. Clin Cardiol 25:II11–II15PubMedGoogle Scholar
  12. Green DJ (2009) Exercise training as vascular medicine: direct impacts on the vasculature in humans. Exerc Sport Sci Rev 37:196–202PubMedGoogle Scholar
  13. Green D, Cheetham C, Mavaddat L, Watts K, Best M, Taylor R, O’Driscoll G (2002a) Effect of lower limb exercise on forearm vascular function: contribution of nitric oxide. Am J Physiol 283:H899–H907Google Scholar
  14. Green D, Cheetham C, Reed C, Dembo L, O’Driscoll G (2002b) Assessment of brachial artery blood flow across the cardiac cycle: retrograde flows during cycle ergometry. J Appl Physiol 93:361–368PubMedGoogle Scholar
  15. Green DJ, Maiorana AJ, Cable NT (2008a) Point: exercise training does induce vascular adaptations beyond the active muscle beds. J Appl Physiol 105:1002–1004 (discussion 1007)PubMedCrossRefGoogle Scholar
  16. Green DJ, O’Driscoll G, Joyner MJ, Cable NT (2008b) Exercise and cardiovascular risk reduction: time to update the rationale for exercise? J Appl Physiol 105:766–768PubMedCrossRefGoogle Scholar
  17. Hambrecht R, Wolf A, Gielen S, Linke A, Hofer J, Erbs S, Schoene N, Schuler G (2000) Effect of exercise on coronary endothelial function in patients with coronary artery disease. N Engl J Med 342:454–460PubMedCrossRefGoogle Scholar
  18. Kazakidi A, Sherwin SJ, Weinberg PD (2009) Effect of Reynolds number and flow division on patterns of haemodynamic wall shear stress near branch points in the descending thoracic aorta. J R Soc Interface 6:539–548PubMedGoogle Scholar
  19. Koskinas KC, Chatzizisis YS, Baker AB, Edelman ER, Stone PH, Feldman CL (2009) The role of low endothelial shear stress in the conversion of atherosclerotic lesions from stable to unstable plaque. Curr Opin Cardiol 24:580–590PubMedCrossRefGoogle Scholar
  20. Ku DN (1997) Blood flow in arteries. Annu Rev Fluid Mech 29:399–434CrossRefGoogle Scholar
  21. Laughlin MH (1995) Endothelium-mediated control of coronary vascular tone after chronic exercise training. Med Sci Sports Exerc 27:1135–1144PubMedGoogle Scholar
  22. Laughlin MH, Newcomer SC, Bender SB (2008) Importance of hemodynamic forces as signals for exercise-induced changes in endothelial cell phenotype. J Appl Physiol 104:588–600PubMedCrossRefGoogle Scholar
  23. Metkus TS, Baughman KL, Thompson PD (2010) Exercise prescription and primary prevention of cardiovascular disease. Circulation 121:2601–2604PubMedCrossRefGoogle Scholar
  24. Mora S, Cook N, Buring JE, Ridker PM, Lee IM (2007) Physical activity and reduced risk of cardiovascular events: potential mediating mechanisms. Circulation 116:2110–2118PubMedCrossRefGoogle Scholar
  25. Nichols WW, O’Rourke MF (2005) McDonald’s blood flow in arteries. Hodder Arnold, LondonGoogle Scholar
  26. Ozawa ET, Bottom KE, Xiao X, Kamm RD (2001) Numerical simulation of enhanced external counterpulsation. Ann Biomed Eng 29:284–297PubMedCrossRefGoogle Scholar
  27. Parker BA, Trehearn TL, Meendering JR (2009a) Last Word on Viewpoint: Pick your Poiseuille: normalizing the shear stimulus in studies of flow-mediated dilation. J Appl Physiol 107:1366CrossRefGoogle Scholar
  28. Parker BA, Trehearn TL, Meendering JR (2009b) Pick your Poiseuille: normalizing the shear stimulus in studies of flow-mediated dilation. J Appl Physiol 107:1357–1359PubMedCrossRefGoogle Scholar
  29. Peacock J, Jones T, Tock C, Lutz R (1998) The onset of turbulence in physiological pulsatile flow in a straight tube. Exp Fluids 24:1–9CrossRefGoogle Scholar
  30. Pyke KE, Tschakovsky ME (2007) Peak vs. total reactive hyperemia: which determines the magnitude of flow-mediated dilation? J Appl Physiol 102:1510–1519PubMedCrossRefGoogle Scholar
  31. Radegran G (1997) Ultrasound Doppler estimates of femoral artery blood flow during dynamic knee extensor exercise in humans. J Appl Physiol 83:1383–1388PubMedGoogle Scholar
  32. Radegran G, Saltin B (1998) Muscle blood flow at onset of dynamic exercise in humans. Am J Physiol 274:H314–H322PubMedGoogle Scholar
  33. Radegran G, Saltin B (2000) Human femoral artery diameter in relation to knee extensor muscle mass, peak blood flow, and oxygen uptake. Am J Physiol 278:H162–H167Google Scholar
  34. Rakobowchuk M, Tanguay S, Burgomaster KA, Howarth KR, Gibala MJ, MacDonald MJ (2008) Sprint interval and traditional endurance training induce similar improvements in peripheral arterial stiffness and flow-mediated dilation in healthy humans. Am J Physiol Regul Integr Comp Physiol 295:R236–R242PubMedCrossRefGoogle Scholar
  35. Rosamond W, Flegal K, Friday G, Furie K, Go A, Greenlund K, Haase N, Ho M, Howard V, Kissela B, Kittner S, Lloyd-Jones D, McDermott M, Meigs J, Moy C, Nichol G, O’Donnell CJ, Roger V, Rumsfeld J, Sorlie P, Steinberger J, Thom T, Wasserthiel-Smoller S, Hong Y (2007) Heart disease and stroke statistics—2007 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Circulation 115:e69–e171PubMedCrossRefGoogle Scholar
  36. Shahcheraghi N, Dwyer HA, Cheer AY, Barakat AI, Rutaganira T (2002) Unsteady and three-dimensional simulation of blood flow in the human aortic arch. J Biomech Eng 124:378–387PubMedCrossRefGoogle Scholar
  37. Simon AC, Levenson J, Flaud P (1990) Pulsatile flow and oscillating wall shear stress in the brachial artery of normotensive and hypertensive subjects. Cardiovasc Res 24:129–136PubMedCrossRefGoogle Scholar
  38. Taguchi I, Ogawa K, Kanaya T, Matsuda R, Kuga H, Nakatsugawa M (2004) Effects of enhanced external counterpulsation on hemodynamics and its mechanism. Circ J 68:1030–1034PubMedCrossRefGoogle Scholar
  39. Tanaka H, Shimizu S, Ohmori F, Muraoka Y, Kumagai M, Yoshizawa M, Kagaya A (2006) Increases in blood flow and shear stress to nonworking limbs during incremental exercise. Med Sci Sports Exerc 38:81–85PubMedCrossRefGoogle Scholar
  40. Thijssen DH, Hopman MT (2008) Counterpoint: exercise training does not induce vascular adaptations beyond the active muscle beds. J Appl Physiol 105:1004–1006 (discussion 1006–1007)PubMedCrossRefGoogle Scholar
  41. Thijssen DH, Dawson EA, Black MA, Hopman MT, Cable NT, Green DJ (2009a) Brachial artery blood flow responses to different modalities of lower limb exercise. Med Sci Sports Exerc 41:1072–1079PubMedCrossRefGoogle Scholar
  42. Thijssen DH, Dawson EA, Tinken TM, Cable NT, Green DJ (2009b) Retrograde flow and shear rate acutely impair endothelial function in humans. Hypertension 53:986–992PubMedCrossRefGoogle Scholar
  43. Tinken TM, Thijssen DH, Hopkins N, Black MA, Dawson EA, Minson CT, Newcomer SC, Laughlin MH, Cable NT, Green DJ (2009) Impact of shear rate modulation on vascular function in humans. Hypertension 54:278–285PubMedCrossRefGoogle Scholar
  44. Tinken TM, Thijssen DH, Hopkins N, Dawson EA, Cable NT, Green DJ (2010) Shear stress mediates endothelial adaptations to exercise training in humans. Hypertension 55:312–318PubMedCrossRefGoogle Scholar
  45. Wisloff U, Stoylen A, Loennechen JP, Bruvold M, Rognmo O, Haram PM, Tjonna AE, Helgerud J, Slordahl SA, Lee SJ, Videm V, Bye A, Smith GL, Najjar SM, Ellingsen O, Skjaerpe T (2007) Superior cardiovascular effect of aerobic interval training versus moderate continuous training in heart failure patients: a randomized study. Circulation 115:3086–3094PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Department of Applied Physiology and Kinesiology, Center for Exercise Science, College of Health and Human PerformanceUniversity of FloridaGainesvilleUSA

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