European Journal of Applied Physiology

, Volume 112, Issue 10, pp 3547–3558 | Cite as

Serum skeletal troponin I following inspiratory threshold loading in healthy young and middle-aged men

  • Glen E. Foster
  • Jiro Nakano
  • A. William  Sheel
  • Jeremy A. Simpson
  • Jeremy D. Road
  • W. Darlene  ReidEmail author
Original Article


The purpose of this study was to determine if serum levels of skeletal troponin I (sTnI, fast and slow isoforms) could provide a sensitive marker of respiratory muscle damage in healthy humans subjected to inspiratory loads. To accomplish this, we studied healthy, young (27 ± 2 years, Mean ± SEM, n = 5) and middle-aged (55 ± 5, n = 5) men to (1) determine the magnitude, pattern, and time course of the presence of sTnI in the serum after a single 60 min bout of inspiratory threshold loading [ITL, ~70% of maximal inspiratory pressure (MIP)], (2) determine the distribution and magnitude of DOMS after loading, and (3) compare fast and slow sTnI levels, and their relationship to other markers/indices of muscle injury including delayed onset muscle soreness (DOMS), serum creatine kinase (CK) levels, and force generating capacity of the respiratory muscles [MIP and maximal expiratory pressure (MEP)]. There was a 24 ± 4 and 27 ± 3% increase in fast sTnI 1 hour (p < 0.01) and 3 days (p < 0.01) after ITL. Slow sTnI was elevated by 24 ± 10% (p < 0.05) 4 days postITL. Other indices of respiratory muscle injury such as CK, MIP and MEP did not show a difference in mean data following ITL; DOMS was slightly but significantly increased following ITL. Our results suggest that sTnI has superior sensitivity compared to other biomarkers/indices of skeletal muscle injury. Future studies examining the impact of respiratory muscle injury with ventilator weaning should consider using sTnI as a sensitive marker of skeletal muscle injury.


Biomarker Diaphragm Exercise Hypoxia Muscle 



We thank our subjects for volunteering their time to complete this research. Primary monoclonal antibody, MYNT-S, was provided to us courtesy of N. Matsumoto. We extend gratitude for the technical and administrative support provided by Ms. Jennifer Rurak and Dr. Cristiane Yamabayashi. This research was supported by a grant-in-aid from the British Columbia Lung Association. G.E. Foster was supported by a fellowship from the Natural Sciences and Engineering Research Council of Canada. A.W. Sheel was supported by a New Investigator award from the Canadian Institutes of Health Research.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Glen E. Foster
    • 1
  • Jiro Nakano
    • 2
  • A. William  Sheel
    • 1
  • Jeremy A. Simpson
    • 5
  • Jeremy D. Road
    • 6
  • W. Darlene  Reid
    • 3
    • 4
    Email author
  1. 1.School of KinesiologyUniversity of British ColumbiaVancouverCanada
  2. 2.Unit of Physical Therapy and Occupational Therapy SciencesGraduate School of Biomedical Sciences, Nagasaki UniversityNagasakiJapan
  3. 3.Department of Physical Therapy, Vancouver Coastal Health Research InstituteUniversity of British ColumbiaVancouverCanada
  4. 4.Muscle Biophysics LaboratoryVancouver Coastal Health AuthorityVancouverCanada
  5. 5.Human Health and Nutritional SciencesUniversity of GuelphGuelphOntario
  6. 6.Division of Respiratory Medicine, Department of MedicineUniversity of British ColumbiaVancouverCanada

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