European Journal of Applied Physiology

, Volume 112, Issue 8, pp 3045–3052 | Cite as

Vitamin D supplementation during exercise training does not alter inflammatory biomarkers in overweight and obese subjects

  • Andres E. Carrillo
  • Michael G. Flynn
  • Catherine Pinkston
  • Melissa M. Markofski
  • Yan Jiang
  • Shawn S. Donkin
  • Dorothy Teegarden
Original Article

Abstract

The purpose of this study was to examine the effects of vitamin D supplementation on inflammatory biomarkers in overweight and obese adults participating in a progressive resistance exercise training program. Twenty-three (26.1 ± 4.7 years) overweight and obese (BMI 31.3 ± 3.2 kg/m2) adults were randomized into a double-blind vitamin D supplementation (Vit D 4,000 IU/day; female 5, male 5) or placebo (PL, female 7; male 6) intervention trial. Both groups performed 12 weeks (3 days/week) of progressive resistance exercise training (three sets of eight exercises) at 70–80% of one repetition maximum. Whole-blood lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNF) α production as well as circulating C-reactive protein (CRP), TNFα, interleukin 6 (IL-6), and alanine aminotransferase (ALT) were assessed at baseline and after the 12-week intervention. No main effects of group or time were detected for circulating CRP, TNFα, IL-6, and ALT. As expected, when PL and Vit D groups were combined, there was a significant correlation between percent body fat and CRP at baseline (r = 0.45, P = 0.04), and between serum 25OHD and CRP at 12 weeks (r = 0.49, P = 0.03). The PL group had a significant increase in 25 μg/ml LPS + polymixin B-stimulated TNFα production (P = 0.04), and both groups had a significant reduction in unstimulated TNFα production (P < 0.05) after the 12-week intervention. Vitamin D supplementation in healthy, overweight, and obese adults participating in a resistance training intervention did not augment exercise-induced changes in inflammatory biomarkers.

Keywords

25-hydroxyvitamin D Resistance training Inflammation Tumor necrosis factor α C-reactive protein Cytokine 

Notes

Acknowledgments

We would like thank our phlebotomist Douglas Maish, as well as Krysta Rickey, Elizabeth Kuhns, Lauren Wagner, and Jessica Harris for assisting with data collection, analysis, and for guiding and motivating participants during training sessions. This research was supported by Gatorade Sports Science Institute and by the National Institutes of Health, National Cancer Institute R25CA128770 (D. Teegarden) Cancer Prevention Internship Program (Yan Jiang) administered by the Oncological Sciences Center and the Discovery Learning Research Center at Purdue University.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Andres E. Carrillo
    • 1
  • Michael G. Flynn
    • 2
  • Catherine Pinkston
    • 3
  • Melissa M. Markofski
    • 4
  • Yan Jiang
    • 3
  • Shawn S. Donkin
    • 5
  • Dorothy Teegarden
    • 3
  1. 1.Wastl Human Performance LaboratoryPurdue UniversityWest LafayetteUSA
  2. 2.Department of Health and Human PerformanceCollege of CharlestonCharlestonUSA
  3. 3.Nutrition SciencePurdue UniversityWest LafayetteUSA
  4. 4.Sealy Center on AgingUniversity of Texas Medical BranchGalvestonUSA
  5. 5.Department of Animal SciencesPurdue UniversityWest LafayetteUSA

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