European Journal of Applied Physiology

, Volume 112, Issue 8, pp 3045–3052 | Cite as

Vitamin D supplementation during exercise training does not alter inflammatory biomarkers in overweight and obese subjects

  • Andres E. Carrillo
  • Michael G. Flynn
  • Catherine Pinkston
  • Melissa M. Markofski
  • Yan Jiang
  • Shawn S. Donkin
  • Dorothy Teegarden
Original Article


The purpose of this study was to examine the effects of vitamin D supplementation on inflammatory biomarkers in overweight and obese adults participating in a progressive resistance exercise training program. Twenty-three (26.1 ± 4.7 years) overweight and obese (BMI 31.3 ± 3.2 kg/m2) adults were randomized into a double-blind vitamin D supplementation (Vit D 4,000 IU/day; female 5, male 5) or placebo (PL, female 7; male 6) intervention trial. Both groups performed 12 weeks (3 days/week) of progressive resistance exercise training (three sets of eight exercises) at 70–80% of one repetition maximum. Whole-blood lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNF) α production as well as circulating C-reactive protein (CRP), TNFα, interleukin 6 (IL-6), and alanine aminotransferase (ALT) were assessed at baseline and after the 12-week intervention. No main effects of group or time were detected for circulating CRP, TNFα, IL-6, and ALT. As expected, when PL and Vit D groups were combined, there was a significant correlation between percent body fat and CRP at baseline (r = 0.45, P = 0.04), and between serum 25OHD and CRP at 12 weeks (r = 0.49, P = 0.03). The PL group had a significant increase in 25 μg/ml LPS + polymixin B-stimulated TNFα production (P = 0.04), and both groups had a significant reduction in unstimulated TNFα production (P < 0.05) after the 12-week intervention. Vitamin D supplementation in healthy, overweight, and obese adults participating in a resistance training intervention did not augment exercise-induced changes in inflammatory biomarkers.


25-hydroxyvitamin D Resistance training Inflammation Tumor necrosis factor α C-reactive protein Cytokine 



We would like thank our phlebotomist Douglas Maish, as well as Krysta Rickey, Elizabeth Kuhns, Lauren Wagner, and Jessica Harris for assisting with data collection, analysis, and for guiding and motivating participants during training sessions. This research was supported by Gatorade Sports Science Institute and by the National Institutes of Health, National Cancer Institute R25CA128770 (D. Teegarden) Cancer Prevention Internship Program (Yan Jiang) administered by the Oncological Sciences Center and the Discovery Learning Research Center at Purdue University.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Armstrong L, Balady G, Berry M, Davis S, Davy B, Davy K, Franklin B, Gordon N, Lee I, McConnell T, Myers J, Pizza F, Rowland T, Stewart K, Thompson P, Wallace J (2006) ACSM’s guidelines for exercise testing and prescription, 7th edn. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
  2. Beavers KM, Nicklas BJ (2011) Effects of lifestyle interventions on inflammatory markers in the metabolic syndrome. Front Biosci (Schol Ed) 3:168–177CrossRefGoogle Scholar
  3. Bjorkman MP, Sorva AJ, Tilvis RS (2009) C-reactive protein and fibrinogen of bedridden older patients in a six-month vitamin D supplementation trial. J Nutr Health Aging 13:435–439PubMedCrossRefGoogle Scholar
  4. Blum M, Dolnikowski G, Seyoum E, Harris SS, Booth SL, Peterson J, Saltzman E, Dawson-Hughes B (2008) Vitamin D(3) in fat tissue. Endocrine 33:90–94PubMedCrossRefGoogle Scholar
  5. Cigolini M, Iagulli MP, Miconi V, Galiotto M, Lombardi S, Targher G (2006) Serum 25-hydroxyvitamin D3 concentrations and prevalence of cardiovascular disease among type 2 diabetic patients. Diabetes Care 29:722–724PubMedCrossRefGoogle Scholar
  6. Dandona P, Aljada A, Bandyopadhyay A (2004) Inflammation: the link between insulin resistance, obesity and diabetes. Trends Immunol 25:4–7PubMedCrossRefGoogle Scholar
  7. Donges CE, Duffield R, Drinkwater EJ (2010) Effects of resistance or aerobic exercise training on interleukin-6, C-reactive protein, and body composition. Med Sci Sports Exerc 42:304–313PubMedGoogle Scholar
  8. Fisher G, Hyatt TC, Hunter GR, Oster RA, Desmond RA, Gower BA (2010) Effect of diet with and without exercise training on markers of inflammation and fat distribution in overweight women. Obesity (Silver Spring) 19:1131–1136CrossRefGoogle Scholar
  9. Goldner WS, Stoner JA, Thompson J, Taylor K, Larson L, Erickson J, McBride C (2008) Prevalence of vitamin D insufficiency and deficiency in morbidly obese patients: a comparison with non-obese controls. Obes Surg 18:145–150PubMedCrossRefGoogle Scholar
  10. Greiwe JS, Cheng B, Rubin DC, Yarasheski KE, Semenkovich CF (2001) Resistance exercise decreases skeletal muscle tumor necrosis factor alpha in frail elderly humans. FASEB J 15:475–482PubMedCrossRefGoogle Scholar
  11. Gutierrez DA, Puglisi MJ, Hasty AH (2009) Impact of increased adipose tissue mass on inflammation, insulin resistance, and dyslipidemia. Curr Diab Rep 9:26–32PubMedCrossRefGoogle Scholar
  12. Heaney RP (2000) Vitamin D: how much do we need, and how much is too much? Osteoporos Int 11:553–555PubMedCrossRefGoogle Scholar
  13. Hirschfield GM, Pepys MB (2003) C-reactive protein and cardiovascular disease: new insights from an old molecule. QJM 96:793–807PubMedCrossRefGoogle Scholar
  14. Holick MF (2005) Vitamin D: important for prevention of osteoporosis, cardiovascular heart disease, type 1 diabetes, autoimmune diseases, and some cancers. South Med J 98:1024–1027PubMedCrossRefGoogle Scholar
  15. Jorde R, Sneve M, Torjesen P, Figenschau Y (2010a) No improvement in cardiovascular risk factors in overweight and obese subjects after supplementation with vitamin D3 for 1 year. J Intern Med 267:462–472PubMedCrossRefGoogle Scholar
  16. Jorde R, Sneve M, Torjesen PA, Figenschau Y, Goransson LG, Omdal R (2010b) No effect of supplementation with cholecalciferol on cytokines and markers of inflammation in overweight and obese subjects. Cytokine 50:175–180PubMedCrossRefGoogle Scholar
  17. Jorde R, Sneve M, Torjesen PA, Figenschau Y, Hansen JB, Grimnes G (2010c) No significant effect on bone mineral density by high doses of vitamin D3 given to overweight subjects for one year. Nutr J 9:1PubMedCrossRefGoogle Scholar
  18. Lechleitner M, Herold M, Dzien-Bischinger C, Hoppichler F, Dzien A (2002) Tumour necrosis factor-alpha plasma levels in elderly patients with Type 2 diabetes mellitus-observations over 2 years. Diabet Med 19:949–953PubMedCrossRefGoogle Scholar
  19. Mauricio D, Mandrup-Poulsen T, Nerup J (1996) Vitamin D analogues in insulin-dependent diabetes mellitus and other autoimmune diseases: a therapeutic perspective. Diabetes Metab Rev 12:57–68PubMedGoogle Scholar
  20. May E, Asadullah K, Zugel U (2004) Immunoregulation through 1,25-dihydroxyvitamin D3 and its analogs. Curr Drug Targets Inflamm Allergy 3:377–393PubMedCrossRefGoogle Scholar
  21. McFarlin BK, Flynn MG, Campbell WW, Stewart LK, Timmerman KL (2004) TLR4 is lower in resistance-trained older women and related to inflammatory cytokines. Med Sci Sports Exerc 36:1876–1883PubMedCrossRefGoogle Scholar
  22. Meshkani R, Adeli K (2009) Hepatic insulin resistance, metabolic syndrome and cardiovascular disease. Clin Biochem 42:1331–1346PubMedCrossRefGoogle Scholar
  23. Myrianthefs P, Karatzas S, Venetsanou K, Grouzi E, Evagelopoulou P, Boutzouka E, Fildissis G, Spiliotopoulou I, Baltopoulos G (2003) Seasonal variation in whole blood cytokine production after LPS stimulation in normal individuals. Cytokine 24:286–292PubMedCrossRefGoogle Scholar
  24. Okita K, Nishijima H, Murakami T, Nagai T, Morita N, Yonezawa K, Iizuka K, Kawaguchi H, Kitabatake A (2004) Can exercise training with weight loss lower serum C-reactive protein levels? Arterioscler Thromb Vasc Biol 24:1868–1873PubMedCrossRefGoogle Scholar
  25. Phillips MD, Flynn MG, McFarlin BK, Stewart LK, Timmerman KL (2010) Resistance training at eight-repetition maximum reduces the inflammatory milieu in elderly women. Med Sci Sports Exerc 42:314–325Google Scholar
  26. Romero-Corral A, Sierra-Johnson J, Lopez-Jimenez F, Thomas RJ, Singh P, Hoffmann M, Okcay A, Korinek J, Wolk R, Somers VK (2008) Relationships between leptin and C-reactive protein with cardiovascular disease in the adult general population. Nat Clin Pract Cardiovasc Med 5:418–425PubMedCrossRefGoogle Scholar
  27. Shah A, Mehta N, Reilly MP (2008) Adipose inflammation, insulin resistance, and cardiovascular disease. JPEN J Parenter Enteral Nutr 32:638–644PubMedCrossRefGoogle Scholar
  28. Sitnick M, Bodine SC, Rutledge JC (2009) Chronic high fat feeding attenuates load-induced hypertrophy in mice. J Physiol 587:5753–5765PubMedCrossRefGoogle Scholar
  29. Stewart LK, Flynn MG, Campbell WW, Craig BA, Robinson JP, McFarlin BK, Timmerman KL, Coen PM, Felker J, Talbert E (2005) Influence of exercise training and age on CD14+ cell-surface expression of toll-like receptor 2 and 4. Brain Behav Immun 19:389–397PubMedCrossRefGoogle Scholar
  30. Stewart LK, Flynn MG, Campbell WW, Craig BA, Robinson JP, Timmerman KL, McFarlin BK, Coen PM, Talbert E (2007) The influence of exercise training on inflammatory cytokines and C-reactive protein. Med Sci Sports Exerc 39:1714–1719PubMedCrossRefGoogle Scholar
  31. Timmerman KL, Flynn MG, Coen PM, Markofski MM, Pence BD (2008) Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise? J Leukoc Biol 84:1271–1278PubMedCrossRefGoogle Scholar
  32. Timms PM, Mannan N, Hitman GA, Noonan K, Mills PG, Syndercombe-Court D, Aganna E, Price CP, Boucher BJ (2002) Circulating MMP9, vitamin D and variation in the TIMP-1 response with VDR genotype: mechanisms for inflammatory damage in chronic disorders? QJM 95:787–796PubMedCrossRefGoogle Scholar
  33. Topolski TD, LoGerfo J, Patrick DL, Williams B, Walwick J, Patrick MB (2006) The Rapid Assessment of Physical Activity (RAPA) among older adults. Prev Chronic Dis 3:A118PubMedGoogle Scholar
  34. von Hurst PR, Stonehouse W, Coad J (2010) Vitamin D supplementation reduces insulin resistance in South Asian women living in New Zealand who are insulin resistant and vitamin D deficient—a randomised, placebo-controlled trial. Br J Nutr 103:549–555Google Scholar
  35. Wortsman J, Matsuoka LY, Chen TC, Lu Z, Holick MF (2000) Decreased bioavailability of vitamin D in obesity. Am J Clin Nutr 72:690–693PubMedGoogle Scholar
  36. Zheng SX, Vrindts Y, Lopez M, De Groote D, Zangerle PF, Collette J, Franchimont N, Geenen V, Albert A, Reginster JY (1997) Increase in cytokine production (IL-1 beta, IL-6, TNF-alpha but not IFN-gamma, GM-CSF or LIF) by stimulated whole blood cells in postmenopausal osteoporosis. Maturitas 26:63–71PubMedCrossRefGoogle Scholar
  37. Zittermann A, Frisch S, Berthold HK, Gotting C, Kuhn J, Kleesiek K, Stehle P, Koertke H, Koerfer R (2009) Vitamin D supplementation enhances the beneficial effects of weight loss on cardiovascular disease risk markers. Am J Clin Nutr 89:1321–1327PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Andres E. Carrillo
    • 1
  • Michael G. Flynn
    • 2
  • Catherine Pinkston
    • 3
  • Melissa M. Markofski
    • 4
  • Yan Jiang
    • 3
  • Shawn S. Donkin
    • 5
  • Dorothy Teegarden
    • 3
  1. 1.Wastl Human Performance LaboratoryPurdue UniversityWest LafayetteUSA
  2. 2.Department of Health and Human PerformanceCollege of CharlestonCharlestonUSA
  3. 3.Nutrition SciencePurdue UniversityWest LafayetteUSA
  4. 4.Sealy Center on AgingUniversity of Texas Medical BranchGalvestonUSA
  5. 5.Department of Animal SciencesPurdue UniversityWest LafayetteUSA

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