Metabolic and functional effects of beta-hydroxy-beta-methylbutyrate (HMB) supplementation in skeletal muscle
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Beta-hydroxy-beta-methylbutyrate (HMB) is a metabolite derived from leucine. The anti-catabolic effect of HMB is well documented but its effect upon skeletal muscle strength and fatigue is still uncertain. In the present study, male Wistar rats were supplemented with HMB (320 mg/kg per day) for 4 weeks. Placebo group received saline solution only. Muscle strength (twitch and tetanic force) and resistance to acute muscle fatigue of the gastrocnemius muscle were evaluated by direct electrical stimulation of the sciatic nerve. The content of ATP and glycogen in red and white portions of gastrocnemius muscle were also evaluated. The effect of HMB on citrate synthase (CS) activity was also investigated. Muscle tetanic force was increased by HMB supplementation. No change was observed in time to peak of contraction and relaxation time. Resistance to acute muscle fatigue during intense contractile activity was also improved after HMB supplementation. Glycogen content was increased in both white (by fivefold) and red (by fourfold) portions of gastrocnemius muscle. HMB supplementation also increased the ATP content in red (by twofold) and white (1.2-fold) portions of gastrocnemius muscle. CS activity was increased by twofold in red portion of gastrocnemius muscle. These results support the proposition that HMB supplementation have marked change in oxidative metabolism improving muscle strength generation and performance during intense contractions.
KeywordsHMB Muscle contraction Glycogen ATP Fatigue Oxidative metabolism
C.H.J Pinheiro was scholar fellowship of São Paulo Research Foundation, FAPESP [2008/54693-9]. Gerlinger-Romero F, Guimarães-Ferreira L, Nachbar RT and Vitzel KF were scholar fellowship of CAPES.
Conflict of interest
The authors declare they have no conflict of interest.
- Cross DA, Alessi DR, Vandenheede JR et al (1994) The inhibition of glycogen synthase kinase-3 by insulin or insulin-like growth factor 1 in the rat skeletal muscle cell line L6 is blocked by wortmannin, but not by rapamycin: evidence that wortmannin blocks activation of the mitogen-activated protein kinase pathway in L6 cells between Ras and Raf. Biochem J 303(Pt 1):21–26PubMedGoogle Scholar
- da Justa Pinheiro CH, de Queiroz JC, Guimarães-Ferreira L et al (2011) Local Injections of adipose-derived mesenchymal stem cells modulate inflammation and increase angiogenesis ameliorating the dystrophic phenotype in dystrophin-deficient skeletal muscle. Stem Cell Rev. doi: 10.1007/s12015-011-9304-0
- Gerlinger-Romero F, Guimarães-Ferreira L, Giannocco G et al (2011). Chronic supplementation of beta-hydroxy-beta methylbutyrate (HMβ) increases the activity of the GH/IGF-I axis and induces hyperinsulinemia in rats. Growth Horm IGF Res. doi: 10.1016/j.ghir.2010.12.006
- Keppler D, Decker K (1974). Glycogen: determination with amyloglucosidase. In: Methods of enzymatic analysis, vol 3, Chemie International, Deerfield Beach, pp 1127–1131 [2nd English edn, translated from the 3rd German edn (Bergmeyer HU, ed)]Google Scholar
- Kreider RB, Ferreira M, Greenwod M et al (2000) Effects of calcium β-HMB supplementation during training on markers of catabolism, body composition, strength and sprint performance. JEP. 3:48–59Google Scholar
- Pinheiro CH, Vitzel KF, Curi R (2010). Effect of N-acetylcysteine on markers of skeletal muscle injury after fatiguing contractile activity. Scand J Med Sci Sports. doi: 10.1111/j.1600-0838.2010.01143.x
- Portal S, Zadik Z, Rabinowitz J et al (2011). The effect of HMB supplementation on body composition, fitness, hormonal and inflammatory mediators in elite adolescent volleyball players: a prospective randomized, double-blind, placebo-controlled study. Eur J Appl Physiol. doi: 10.1007/s00421-011-1855-x
- Randle PJ, Garland PB, Hales CN et al (1963) The glucose fatty-acid cycle. Its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus. Lancet 1(7285):785–789Google Scholar
- Turner N, Bruce CR, Beale SM et al (2007). Excess lipid availability increases mitochondrial fatty acid oxidative capacity in muscle: evidence against a role for reduced fatty acid oxidation in lipid-induced insulin resistance in rodents. Diabetes (8):2085–2092Google Scholar
- van Koverin M, Nissen SL (1992) Oxidation of leucine and alpha-ketoisocaproate to β-hydroxy-β-methylbutyrate in vivo. Am J Physiol Endocrinol Metab 262:27Google Scholar
- van Someren KA, Edwards AJ, Howatson G (2005) Supplementation with beta-hydroxy-beta-methylbutyrate (HMB) and alpha-ketoisocaproic acid (KIC) reduce signs and symptoms of exercise-induced muscle damage in man. Int J Sport Nutr Exerc Metabol 15:413–424Google Scholar
- Zanchi NE, Gerlinger-Romero F, Guimarães-Ferreira L et al (2010) HMB supplementation: clinical and athletic performance-related effects and mechanisms of action. Amino Acids. doi: 10.1007/s00726-010-0678-0