Swim training does not protect mice from skeletal muscle oxidative damage following a maximum exercise test
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We investigated whether swim training protects skeletal muscle from oxidative damage in response to a maximum progressive exercise. First, we investigated the effect of swim training on the activities of the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), in the gastrocnemius muscle of C57Bl/6 mice, 48 h after the last training session. Mice swam for 90 min, twice a day, for 5 weeks at 31°C (±1°C). The activities of SOD and CAT were increased in trained mice (P < 0.05) compared to untrained group. However, no effect of training was observed in the activity of GPx. In a second experiment, trained and untrained mice were submitted to a maximum progressive swim test. Compared to control mice (untrained, not acutely exercised), malondialdehyde (MDA) levels were increased in the skeletal muscle of both trained and untrained mice after maximum swim. The activity of GPx was increased in the skeletal muscle of both trained and untrained mice, while SOD activity was increased only in trained mice after maximum swimming. CAT activity was increased only in the untrained compared to the control group. Although the trained mice showed increased activity of citrate synthase in skeletal muscle, swim performance was not different compared to untrained mice. Our results show an imbalance in the activities of SOD, CAT and GPx in response to swim training, which could account for the oxidative damage observed in the skeletal muscle of trained mice in response to maximum swim, resulting in the absence of improved exercise performance.
KeywordsSwim Oxidative stress Superoxide dismutase Catalase Glutathione peroxidase Exercise performance
This work was supported by FAPEMIG grant number CDSAPQ-4263-5.01/07.
- Campi-Azevedo AC, Cleto LS, Silva RS, Sousa-Franco J, Magalhães JC, Penaforte CL, Castro Pinto KM, Rocha-Vieira E (2011) Divergent cytokine response following maximum progressive swimming in hot water. Cell Biochem Funct doi: 10.1002/cbf.1795
- Cleto LS, Oleto AF, Sousa LP, Barreto TO, Cruz JS, Penaforte CL, Magalhães JC, Sousa-Franco J, Pinto KM, Campi-Azevedo AC, Rocha-Vieira E (2011) Plasma cytokine response, lipid peroxidation and NF-κB activation in skeletal muscle following maximum progressive swimming. Braz J Med Biol Res 44:546–552Google Scholar
- Fabri T, Machado K, Rezende R, Vieira M, Mercês L, Santos MJC, Rocha-Vieira E, Oliveira LKB (2010) Aquatic and land exercise training affects renal function in rats under isosmotic volume expansion. J Exer Physiol Online 13:42–51Google Scholar
- König D, Wagner KH, Elmadfa I, Berg A (2001) Exercise and oxidative stress: significance of antioxidants with reference to inflammatory, muscular, and systemic stress. Exerc Immunol Rev 7:108–133Google Scholar
- Kuwahara H, Horie T, Ishikawa S, Tsuda C, Kawakami S, Noda Y, Kaneko T, Tahara S, Tachibana T, Okabe M, Melki J, Takano R, Toda T, Morikawa D, Nojiri H, Kurosawa H, Shirasawa T, Shimizu T (2010) Oxidative stress in skeletal muscle causes severe disturbance of exercise activity without muscle atrophy. Free Radic Biol Med 48:1252–1262PubMedCrossRefGoogle Scholar
- Pereira B, Costa Rosa LFB, Safi DA, Medeiros MHG, Curi R, Bechara EJH (1994) Superoxide dismutase, catalase, and glutathione peroxidase activities in muscle and lymphoid organs of sedentary and exercise-trained rats. Physiol Behav 56:1095e9Google Scholar