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European Journal of Applied Physiology

, Volume 112, Issue 2, pp 493–500 | Cite as

Carbohydrate supplementation delays DNA damage in elite runners during intensive microcycle training

  • Maysa Vieira de Sousa
  • Klavs Madsen
  • Rosa Fukui
  • Aritania Santos
  • Maria Elizabeth Rossi da Silva
Original Article

Abstract

The aim of this study was to evaluate the effect of carbohydrate supplementation on free plasma DNA and conventional markers of training and tissue damage in long-distance runners undergoing an overload training program. Twenty-four male runners were randomly assigned to two groups (CHO group and control group). The participants were submitted to an overload training program (days 1–8), followed by a high-intensity intermittent running protocol (10 × 800 m) on day 9. The runners received maltodextrin solution (CHO group) or zero energy placebo solution as the control equivalent before, during, and after this protocol. After 8 days of intensive training, baseline LDH levels remained constant in the CHO group (before: 449.1 ± 18.2, after: 474.3 ± 22.8 U/L) and increased in the control group (from 413.5 ± 23.0 to 501.8 ± 24.1 U/L, p < 0.05). On day 9, LDH concentrations were lower in the CHO group (509.2 ± 23.1 U/L) than in the control group (643.3 ± 32.9 U/L, p < 0.01) post-intermittent running. Carbohydrate ingestion attenuated the increase of free plasma DNA post-intermittent running (48,240.3 ± 5,431.8 alleles/mL) when compared to the control group (73,751.8 ± 11,546.6 alleles/mL, p < 0.01). Leukocyte counts were lower in the CHO group than in the control group post-intermittent running (9.1 ± 0.1 vs. 12.2 ± 0.7 cells/µL; p < 0.01) and at 80 min of recovery (10.6 ± 0.1 vs. 13.9 ± 1.1 cells/µL; p < 0.01). Cortisol levels were positively correlated with free plasma DNA, leukocytes, and LDH (all r > 0.4 and p < 0.001). The results showed that ingestion of a carbohydrate beverage resulted in less DNA damage and attenuated the acute post-exercise inflammation response, providing better recovery during intense training.

Keywords

Free plasma DNA Tissue damage Inflammation Overtraining Intensive training 

Notes

Acknowledgments

The authors thank Niels Pallisgaard from Velje Hospital in Denmark, and Maria José Pegoraro and Greci da Silva Paula from LIM-18, University of São Paulo Medical School, for help with the study. We also thank Claire Neesham and Kerstin Markendorf for proofreading the manuscript, and the State of São Paulo Research Foundation (FAPESP), Brazil, for the fellowship granted. The project was supported by FAPESP.

References

  1. Angeli A, Minetto M, Dovio A, Paccotti P (2004) The overtraining syndrome in athletes: a stress-related disorder. J Endocrinol Invest 27:603–612PubMedGoogle Scholar
  2. Balon TW, Nadler JL (1994) Nitric oxide release is present from incubated skeletal muscle preparations. J Appl Physiol 77:2519–2521PubMedGoogle Scholar
  3. Bassini-Cameron A, Sweet E, Bottino A, Bittar C, Veiga C, Cameron L (2007) Effect of caffeine supplementation on haematological and biochemical variables in elite soccer players under physical stress conditions. Br J Sports Med 41:523–530PubMedCrossRefGoogle Scholar
  4. Bessa A, Nissenbaum M, Monteiro A, Gandra PG, Nunes LS, Bassini-Cameron A, Werneck-de-Castro JPS, de Macedo DV, Cameron LC (2008) High-intensity ultraendurance promotes early release of muscle injury markers. Br J Sports Med 42:889–893PubMedCrossRefGoogle Scholar
  5. Brancaccio P, Maffulli N, Limongelli FM (2007) Creatine kinase monitoring in sport medicine. Br Med Bull 81–82:209–230PubMedCrossRefGoogle Scholar
  6. Chen TC, Hsieh SS (2001) Effects of a 7-day eccentric training period on muscle damage and inflammation. Med Sci Sports Exerc 33:1732–1738PubMedCrossRefGoogle Scholar
  7. Close GL, Ashton T, Cable T, Doran D, Noyes C, McArdle F, MacLaren DPM (2005) Effects of dietary carbohydrate on delayed onset muscle soreness and reactive. Br J Sports Med 39:948–953PubMedCrossRefGoogle Scholar
  8. de Sousa MV, Madsen K, Simoes HG, Pereira RM, Negrao CE, Mendonca RZ, Takayama L, Fukui R, da Silva ME (2010) Effects of carbohydrate supplementation on competitive runners undergoing overload training followed by a session of intermittent exercise. Eur J Appl Physiol 109:507–516PubMedCrossRefGoogle Scholar
  9. Dhabhar FS, McEwen BS (1999) Enhancing versus suppressive effects of stress hormones on skin immune function. Proc Nat Acad Sci 96:1059–1064PubMedCrossRefGoogle Scholar
  10. Fatouros IG, Destouni A, Margonis K, Jamurtas AZ, Vrettou C, Kouretas D, Mastorakos G, Mitrakou A, Taxildaris K, Kanavakis E, Papassotiriou I (2006) Cell-free plasma DNA as a novel marker of aseptic inflammation severity related to exercise overtraining. Clin Chem 52:1820–1821PubMedCrossRefGoogle Scholar
  11. Febbraio M, Pedersen BK (2002) Muscle-derived interleukin-6: mechanisms for activation and possible biological roles. FASEB J 16:1335–1347PubMedCrossRefGoogle Scholar
  12. Flint MS, Baum A, Chambers WH, Jenkins FJ (2007) Induction of DNA damage, alteration of DNA repair and transcriptional activation by stress hormones. Psychoneuroendocrinology 32:470–479PubMedCrossRefGoogle Scholar
  13. Gleeson M (2007) Immune function in sport and exercise. J Appl Physiol 103:693–699PubMedCrossRefGoogle Scholar
  14. Halson SL, Jeukendrup AE (2004) Does overtraining exist? Sports Med 34:967–981PubMedCrossRefGoogle Scholar
  15. Inoue M, Yamamoto S, Kurahashi N, Iwasaki M, Sasazuki S, Tsugane S (2008) Daily total physical activity level and total cancer risk in men and women: results from a large-scale population-based cohort study in Japan, and for the Japan public health center-based prospective study group. Am J Epidemiol 168:391–403PubMedCrossRefGoogle Scholar
  16. Judelson DA, Maresh CM, Yamamoto LM, Farrell MJ, Armstrong LE, Kraemer WJ, Volek JS, Spiering BA, Casa DJ, Anderson JM (2008) Effect of hydration state on resistance exercise-induced endocrine markers of anabolism, catabolism, and metabolism. J Appl Physiol 105:816–824PubMedCrossRefGoogle Scholar
  17. Kenttä G, Hassmén P (1998) Overtraining and recovery. Sports Med 26:1–16PubMedCrossRefGoogle Scholar
  18. Kraemer WJ, Volek JS, Bush JA, Putukian M, Sebastianelli WJ (1998) Hormonal responses to consecutive days of heavy-resistance exercise with or without nutritional supplementation. J Appl Physiol 85:1544–1555PubMedGoogle Scholar
  19. Li Y, Wu H, Khardori R, Song YH, Lu YW, Geng YJ (2009) Insulin-like growth factor-1 receptor activation prevents high glucose-induced mitochondrial dysfunction, cytochrome-c release and apoptosis. Biochem Biophys Res Commun 384:259–264PubMedCrossRefGoogle Scholar
  20. Maughan RJ (1997) Energy and macronutrient intakes of professional soccer players. Br J Sports Med 31:45–47PubMedCrossRefGoogle Scholar
  21. McAnulty S, McAnulty L, Nieman D, Morrow J, Dumke C, Utter A (2007) Carbohydrate effect: hormone and oxidative changes. Int J Sports Med 28:921–927PubMedCrossRefGoogle Scholar
  22. McKenzie DC (1999) Markers of excessive exercise. Can J Appl Physiol 24:66–73PubMedCrossRefGoogle Scholar
  23. Meeusen R, Duclos M, Gleeson M, Rietjens G, Steinacker J, Urhausen A (2006) Prevention, diagnosis and treatment of the overtraining syndrome. Eur J Sport Sci 6:1–14CrossRefGoogle Scholar
  24. Mitchell JB, Pizza FX, Paquet A, Davis BJ, Forrest MB, Braun WA (1998) Influence of carbohydrate status on immune responses before and after endurance exercise. J Appl Physiol 84:1917–1925PubMedGoogle Scholar
  25. Moreira A, Delgado L, Moreira P, Haahtela T (2009) Does exercise increase the risk of upper respiratory tract infections? Br Med Bull 90:111–131PubMedCrossRefGoogle Scholar
  26. Mougios V (2007) Reference intervals for serum creatine kinase in athletes. Br J Sports Med 41:674–678PubMedCrossRefGoogle Scholar
  27. Nikolaidis MG, Jamurtas AZ, Paschalis V, Fatouros IG, Koutedakis Y, Kouretas D (2008) The effect of muscle-damaging exercise on blood and skeletal muscle oxidative stress. Sports Med 38:579–606PubMedCrossRefGoogle Scholar
  28. Overgaard K, Fredsted A, Hyldal A, Ingemann-Hansen T, Gissel H, Clausen T (2004) Effects of running distance and training on Ca2+ content and damage in human muscle. Med Sci Sports Exerc 36:821–829PubMedCrossRefGoogle Scholar
  29. Pedersen BK, Hoffman-Goetz L (2000) Exercise and the immune system: regulation, integration, and adaptation. Physiol Rev 80:1055–1081PubMedGoogle Scholar
  30. Romano-Ely BC, Todd MK, Saunders MJ, Laurent TS (2006) Effect of an isocaloric carbohydrate–protein-antioxidant drink on cycling performance. Med Sci Sports Exerc 38:1608–1616PubMedCrossRefGoogle Scholar
  31. Rose AJ, Richter EA (2005) Skeletal muscle glucose uptake during exercise: how is it regulated? Physiology 20:260–270PubMedCrossRefGoogle Scholar
  32. Saunders MJ, Kane MD, Todd MK (2004) Effects of a carbohydrate–protein beverage on cycling endurance and muscle damage. Med Sci Sports Exerc 36:1233–1238PubMedCrossRefGoogle Scholar
  33. Scharhag J, Meyer T, Auracher M, Gabriel HH, Kindermann W (2006) Effects of graded carbohydrate supplementation on the immune response in cycling. Med Sci Sports Exerc 38:286–292PubMedCrossRefGoogle Scholar
  34. Stroun M, Lyautey J, Lederrey C, Olson-Sand A, Anker P (2001) About the possible origin and mechanism of circulating DNA: apoptosis and active DNA release. Clin Chim Acta 313:139–142PubMedCrossRefGoogle Scholar
  35. Su Q, Tian Y, Zhang JG, Zhang H (2008) Effects of allicin supplementation on plasma markers of exercise-induced muscle damage, IL-6 and antioxidant capacity. Eur J Appl Physiol 103:275–283PubMedCrossRefGoogle Scholar
  36. Sugiura K, Suzuki I, Kobayashi K (1999) Nutritional intake of elite Japanese track-and-field athletes. Int J Sport Nutr 9:202–212PubMedGoogle Scholar
  37. Sun X, Yasuda O, Takemura Y, Kawamoto H, Higuchi M, Baba Y, Katsuya T, Fukuo K, Ogihara T, Rakugi H (2008) Akt activation prevents Apop-1-induced death of cells. Biochem Biophys Res Commun 377:1097–1101PubMedCrossRefGoogle Scholar
  38. Suzuki K, Totsuka M, Nakaji S, Yamada M, Kudoh S, Liu Q, Sugawara K, Yamaya K, Sato K (1999) Endurance exercise causes interaction among stress hormones, cytokines, neutrophil dynamics, and muscle damage. J Appl Physiol 87:1360–1367PubMedGoogle Scholar
  39. Swaminathan R, Butt AN (2006) Circulating nucleic acids in plasma and serum: recent developments. Ann N Y Acad Sci 1075:1–9PubMedCrossRefGoogle Scholar
  40. Teshima Y, Takahashi N, Thuc LC, Nishio S, Nagano-Torigoe Y, Miyazaki H, Ezaki K, Yufu K, Hara M, Nakagawa M, Saikawa T (2010) High-glucose condition reduces cardioprotective effects of insulin against mechanical stress-induced cell injury. Life Sci 87:154–161PubMedCrossRefGoogle Scholar
  41. Totsuka M, Nakaji S, Suzuki K, Sugawara K, Sato K (2002) Break point of serum creatine kinase release after endurance exercise. J Appl Physiol 93:1280–1286PubMedGoogle Scholar
  42. Tsai K, Hsu T, Hsu K, Cheng H, Liu T, Hsu C, Kong C (2001) Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise. Free Radic Biol Med 31:1465–1472PubMedCrossRefGoogle Scholar
  43. van der Vaart M, Pretorius PJ (2008) Circulating DNA—its origin and fluctuation. Ann N Y Acad Sci 1137:18–26PubMedCrossRefGoogle Scholar
  44. Ziegler PJ, Nelson JA, Jonnalagadda SS (1999) Nutritional and physiological status of US national figure skating. Int J Sports Nutr 9:345–360Google Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Maysa Vieira de Sousa
    • 1
  • Klavs Madsen
    • 2
  • Rosa Fukui
    • 1
  • Aritania Santos
    • 1
  • Maria Elizabeth Rossi da Silva
    • 1
  1. 1.Laboratory of Medical Investigation LIM-18, Faculty of MedicineUniversity of São PauloSão PauloBrazil
  2. 2.Department of Sport ScienceAarhus UniversityAarhusDenmark

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