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European Journal of Applied Physiology

, Volume 112, Issue 1, pp 387–396 | Cite as

Alterations in muscular oxidative metabolism parameters in incremental treadmill exercise test in untrained rats

  • Ricardo A. Pinho
  • Luciano D. Silva
  • Cleber A. Pinho
  • Juliana F. Daufenbach
  • Gislaine T. Rezin
  • Luciano A. da Silva
  • Emílio L. Streck
  • Claudio T. Souza
Original Article

Abstract

The present study investigates the effects of incremental exercise test on muscular oxidative metabolism. Thirty-six 2-month-old male Wistar rats were distributed in seven groups that performed exercise at different levels: first level (control), second level (0.6 km/h), third level (0.6 and 0.8 km/h), fourth level (0.6, 0.8 and 1.0 km/h), fifth level (0.6, 0.8, 1.0 and 1.2 km/h), sixth level (0.6, 0.8, 1.0, 1.2 and 1.4 km/h), and seventh level (0.6, 0.8, 1.0, 1.2, 1.4 and 1.6 km/h). At the end of the exercise challenge, level of blood lactate (BL), glycogen content (MG), creatine kinase (CK), complexes (CI, CII, CIII, CIV), oxidative damage, succinate dehydrogenase (SDH), cytochrome c oxidase as well as antioxidant enzymes (SOD and CAT) expression were measured. The speed of 1.0 km/h increased BL level, while 1.2 km/h decreased MG and increased serum CK. Increased SDH expression was observed after intensity levels 6 and 7, and cytochrome c oxidase expression increased after levels 5, 6 and 7, in comparison with lower intensity levels, ETC enzyme activities increased when exercise was applied at intensities of 0.8 km/h (CI), 1.0 km/h (CII and CIII), and 1.2 km/h (CIV). The increase in SOD expression did not occur as observed for superoxide production, except for rats that underwent exercise at level 7, but CAT expression increased significantly in all levels, starting from level 3. Our results show interesting alterations in the muscular metabolism parameters, and suggest a differential response of muscle oxidative metabolism when intense exercise is applied at different speeds.

Keywords

Physical exercise Effort intensity Skeletal muscle Electron transport chain 

Notes

Acknowledgments

This research was supported by grants from CNPq/MCT (Brazil), CAPES/MEC (Brazil) and UNESC (Brazil).

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ricardo A. Pinho
    • 1
  • Luciano D. Silva
    • 1
  • Cleber A. Pinho
    • 1
  • Juliana F. Daufenbach
    • 2
  • Gislaine T. Rezin
    • 2
  • Luciano A. da Silva
    • 1
  • Emílio L. Streck
    • 2
  • Claudio T. Souza
    • 1
  1. 1.Exercise Biochemistry and Physiology Laboratory, Postgraduate Program in Health Sciences, Health Sciences UnitUniversidade do Extremo Sul CatarinenseCriciúmaBrazil
  2. 2.Experimental Physiopathology Laboratory, Postgraduate Program in Health Sciences, Health Sciences UnitUniversidade do Extremo Sul CatarinenseCriciúmaBrazil

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