European Journal of Applied Physiology

, Volume 112, Issue 1, pp 357–364 | Cite as

Effects of aerobic exercise on urinary estrogens and progestagens in pre and postmenopausal women

  • María Concepción Robles GilEmail author
  • R. Timón
  • A. F. Toribio
  • D. Muñoz
  • J. I. Maynar
  • M. J. Caballero
  • M. Maynar
Original Article


The purpose of this study was to elucidate the effect of 6 months of aerobic exercise on urinary excretion of female steroid hormones in pre and postmenopausal women and to check the basal values of urinary steroid. To this end, 20 premenopausal (age 45.56 ± 4.06 years) and 20 postmenopausal (age 52.27 ± 3.80 years) women, all sedentary, were studied before and after a supervised 6-month exercise training program (at 60–70% of maximal heart rate, 60 min/day, 3 days/week), based on aerobic dance. The exercise included standing on one leg, squatting, walking, and touching their heels. Before and after the program, anthropometric data and VO2max were measured and urine samples were collected and analyzed by gas chromatography/mass spectrometry (GC/Q-MS). Both, pre and postmenopausal women, improved their VO2max after the aerobic exercise program. Regarding the urinary steroids, on the one hand, important differences were observed between urinary estrogens and progestagens in pre and postmenopausal women in basal values. Estrone (P < 0.05), pregnanediol (P < 0.01), pregnanetriol (P < 0.05), and estriol (P < 0.01) levels were lower in postmenopausal women than in premenopausal women. On the other hand, the aerobic exercise program did not affect postmenopausal women in the same way as premenopausal women. After the exercise program, no changes in urinary steroid levels were observed in premenopausal women. However, the aerobic exercise program caused an increase in urinary excretion of pregnanediol (P < 0.05) and pregnanetriol (P < 0.05) in postmenopausal women.


Urinary estrogens Urinary progestagens Menopause Aerobic exercise 



This work was supported by the University of Extremadura (Spain) [OR06/A7-07 and A2-37].

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • María Concepción Robles Gil
    • 1
    • 5
    Email author
  • R. Timón
    • 1
  • A. F. Toribio
    • 2
  • D. Muñoz
    • 1
  • J. I. Maynar
    • 2
  • M. J. Caballero
    • 3
  • M. Maynar
    • 4
  1. 1.Department of Physical Education and Sport, Sport Sciences FacultyUniversity of ExtremaduraBadajozSpain
  2. 2.Department of Analytical Chemistry, Science FacultyUniversity of ExtremaduraBadajozSpain
  3. 3.Unit of Pharmacology, School of MedicineUniversity of ExtremaduraBadajozSpain
  4. 4.Department of Physiology, Sport Sciences FacultyUniversity of ExtremaduraBadajozSpain
  5. 5.CáceresSpain

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