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European Journal of Applied Physiology

, Volume 112, Issue 1, pp 357–364 | Cite as

Effects of aerobic exercise on urinary estrogens and progestagens in pre and postmenopausal women

  • María Concepción Robles GilEmail author
  • R. Timón
  • A. F. Toribio
  • D. Muñoz
  • J. I. Maynar
  • M. J. Caballero
  • M. Maynar
Original Article

Abstract

The purpose of this study was to elucidate the effect of 6 months of aerobic exercise on urinary excretion of female steroid hormones in pre and postmenopausal women and to check the basal values of urinary steroid. To this end, 20 premenopausal (age 45.56 ± 4.06 years) and 20 postmenopausal (age 52.27 ± 3.80 years) women, all sedentary, were studied before and after a supervised 6-month exercise training program (at 60–70% of maximal heart rate, 60 min/day, 3 days/week), based on aerobic dance. The exercise included standing on one leg, squatting, walking, and touching their heels. Before and after the program, anthropometric data and VO2max were measured and urine samples were collected and analyzed by gas chromatography/mass spectrometry (GC/Q-MS). Both, pre and postmenopausal women, improved their VO2max after the aerobic exercise program. Regarding the urinary steroids, on the one hand, important differences were observed between urinary estrogens and progestagens in pre and postmenopausal women in basal values. Estrone (P < 0.05), pregnanediol (P < 0.01), pregnanetriol (P < 0.05), and estriol (P < 0.01) levels were lower in postmenopausal women than in premenopausal women. On the other hand, the aerobic exercise program did not affect postmenopausal women in the same way as premenopausal women. After the exercise program, no changes in urinary steroid levels were observed in premenopausal women. However, the aerobic exercise program caused an increase in urinary excretion of pregnanediol (P < 0.05) and pregnanetriol (P < 0.05) in postmenopausal women.

Keywords

Urinary estrogens Urinary progestagens Menopause Aerobic exercise 

Notes

Acknowledgments

This work was supported by the University of Extremadura (Spain) [OR06/A7-07 and A2-37].

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Al-Azzawi F, Palacios S (2009) Hormonal changes during menopause. Maturitas 63:135–137PubMedCrossRefGoogle Scholar
  2. Asikainen TM, Miilunpalo S, Oja P, Rinne M, Pasanen M, Vuori I (2002a) Walking trials in postmenopausal women: effect of one vs two daily bouts on aerobic fitness. Scand J Med Sci Sports 12:99–105PubMedCrossRefGoogle Scholar
  3. Asikainen TM, Miilunpalo S, Oja P, Rinne M, Pasanen M, Uusi-Rasi K, Vuori I (2002b) Randomised, controlled walking trials in postmenopausal women: the minimum dose to improve aerobic fitness? Br J Sports Med 36:189–194PubMedCrossRefGoogle Scholar
  4. Asikainen TM, Kukkonen-Harjula K, Miilunpalo S (2004) Exercise for health for early postmenopausal women: a systematic review of randomised controlled trials. Sports Med 34(11):753–778PubMedCrossRefGoogle Scholar
  5. Atkinson C, Lampe JW, Tworoger SS, Ulrich CM, Bowen D, Irwin ML, Schwartz RS, Rajan BK, Yasui Y, Potter JD, McTiernan A (2004) Effects of a moderate intensity exercise intervention on estrogen metabolism in postmenopausal women. Cancer Epidemiol Biomarkers Prev 13:868–874PubMedGoogle Scholar
  6. Baglietto L, English DR, Hopper JL, MacInnis RJ, Morris HA, Tilley WD, Krishnan K, Giles GG (2009) Circulating steroid hormone concentrations in postmenopausal women in relation to body size and composition. Breast Cancer Res Treat 115:171–179PubMedCrossRefGoogle Scholar
  7. Bertone-Johnson ER, Tworoger SS, Hankinson SE (2009) Recreational physical activity and steroid hormone levels in postmenopausal women. Am J Epidemiol 170(9):1095–1104 [Epub 2009 Sep 25]PubMedCrossRefGoogle Scholar
  8. Broocks A, Pirke KM, Schweiger U, Tuschl RJ, Laessle RG, Strowitzki T, Horl E, Horl T, Haas W, Jeschke D (1990) Cyclic ovarian function in recreational athletes. J Appl Physiol 68:2083–2086PubMedGoogle Scholar
  9. Brooke-Wavell K, Jones PR, Hardman AE (1997) Brisk walking reduces calcaneal bone loss in post-menopausal women. Clin Sci (Lond) 92:75–80Google Scholar
  10. Brožek J, Grande F, Anderson JT, Keys A (1963) Densitometric analysis of body composition: revision of some quantitive assumptions. Ann N Y Acad Sci 101:113–140Google Scholar
  11. Bryant HU, Fukuyama T, Nakazawa T (2000) Raloxifene mimics the effect of estrogen on bone. Tanpakushitsu Kakusan Koso 45:1102–1108PubMedGoogle Scholar
  12. Bullen BA, Skrinar GS, Beitins IZ, Carr DB, Reppert SM, Dotson CO, Fencl MD, Gervino EV, McArthur JW (1984) Endurance training effects on plasma hormonal responsiveness and sex hormone excretion. J Appl Physiol 56:1453–1463PubMedGoogle Scholar
  13. Chen KT, Yang RS (2004) Effects of exercise on lipid metabolism and musculoskeletal fitness in female athletes. World J Gastroenterol 10(1):122–126PubMedGoogle Scholar
  14. Church TS, Earnest CP, Skinner JS, Blair SN (2007) Effects of different doses of physical activity on cardiorespiratory fitness among sedentary, overweight or obese postmenopausal women with elevated blood pressure: a randomized controlled trial. Jama 297:2081–2091PubMedCrossRefGoogle Scholar
  15. Ciolac EG, Bocchi EA, Bortolotto LA, Carvalho VO, Greve JM, Guimarães GV (1998) Effects of high-intensity aerobic interval training vs. moderate exercise on hemodynamic, metabolic and neuro-humoral abnormalities of young normotensive women at high familial risk for hypertension. Hypertens Res 33(8):836–843CrossRefGoogle Scholar
  16. De Rose EH, Guimaraes AGS (1980) A model for optimization of somatotype in young athletes. In: M. Ostyn, G. Beunen, J. Simons (Eds) Kinanthropometry II. University Park Press, Baltimore, pp 77–80Google Scholar
  17. Desai MP, Bhanuprakash KV, Khatkhatay MI, Donde UM (2007) Age-related changes in bone turnover markers and ovarian hormones in premenopausal and postmenopausal Indian women. J Clin Lab Anal 21:55–60PubMedCrossRefGoogle Scholar
  18. Durnin JV, Womersley J (1974) Body fat content assessed from total body density and its estimation from skinfold thickness: measurements on 481 men and women aged from 16 to 72 years. Br J Nutr 32:77–97PubMedCrossRefGoogle Scholar
  19. Ellison PT, Lager C (1986) Moderate recreational running is associated with lowered salivary progesterone profiles in women. Am J Obstet Gynecol 154:1000–1003PubMedGoogle Scholar
  20. Falk R, Gail M, Fears T, Rossi S, Stanczyk F, Adlercreutz H, Kiura P, Wahala K, Donaldson JL, Vaught J, Fillmore C, Hoover R, Ziegler R (1999) Reproducibility and validity of radioimmunoassays for urinary hormones and metabolites in pre- and postmenopausal women. Cancer Epidemiol Biomarkers Prev 8:C567–C577Google Scholar
  21. Friedenreich CM, Orenstein MR (2002) Physical activity and cancer prevention: etiologic evidence and biological mechanisms. J Nutr 132:3456S–3464SPubMedGoogle Scholar
  22. Friedlander AL, Casazza GA, Horning MA, Huie MJ, Piacentini MF, Trimmer JK, Brooks GA (1998) Training-induced alterations of carbohydrate metabolism in women: women respond differently from men. J Appl Physiol 85:1175–1186PubMedGoogle Scholar
  23. Hughes DC and Runyan SJ (1995) Prenatal care and public policy: lessons for promoting women’s health. J Am Med Womens Assoc 50:156–159, 163Google Scholar
  24. IUPAC, Analytical chemistry division (1978) Nomenclature, symbol, units and their usage in spectrochemical analysis. II. Data interpretation. Spectrochim Acta B 33:241–246Google Scholar
  25. Jakicic JM, Marcus BH, Gallagher KI, Napolitano M, Lang W (2003) Effect of exercise duration and intensity on weight loss in overweight, sedentary women: a randomized trial. Jama 290:1323–1330PubMedCrossRefGoogle Scholar
  26. Keizer HA, Kuipers H, de Haan J, Janssen GM, Beckers E, Habets L, van Kranenburg G, Geurten P (1987) Effect of a 3-month endurance training program on metabolic and multiple hormonal responses to exercise. Int J Sports Med 8(Suppl 3):154–160PubMedCrossRefGoogle Scholar
  27. Kemmler W, Lauber D, Weineck J, Hensen J, Kalender W, Engelke K (2004) Benefits of 2 years of intense exercise on bone density, physical fitness, and blood lipids in early postmenopausal osteopenic women: results of the Erlangen Fitness Osteoporosis Prevention Study (EFOPS). Arch Intern Med 164:1084–1091PubMedCrossRefGoogle Scholar
  28. Liu JH, Kao L, Rebar RW, Muse K (2003) Urinary beta-FSH subunit concentrations in perimenopausal and postmenopausal women: a biomarker for ovarian reserve. Menopause 10:526–533PubMedCrossRefGoogle Scholar
  29. Magkos F, Fabbrini E, Mohammed BS, Patterson BW, Klein S, Mittendorfer B (2010) Estrogen deficiency after menopause does not result in male very-low-density lipoprotein metabolism phenotype. J Clin Endocrinol Metab 95(7):3377–3384 [Epub 2010 May 5]PubMedCrossRefGoogle Scholar
  30. Morris FL, Wark JD (2001) An effective, economic way of monitoring menstrual cycle hormones in at risk female athletes. Med Sci Sports Exerc 33(1):9–14PubMedCrossRefGoogle Scholar
  31. Morris FL, Payne WR, Wark JD (1999a) Prospective decrease in progesterone concentrations in female lightweight rowers during the competition season compared with the off season: a controlled study examining weight loss and intensive exercise. Br J Sports Med 33(6):417–422PubMedCrossRefGoogle Scholar
  32. Morris FL, Payne WR, Wark JD (1999b) The impact of intense training on endogenous estrogen and progesterone concentrations and bone mineral acquisition in adolescent rowers. Osteoporos Int 10:361–368PubMedCrossRefGoogle Scholar
  33. Munro CJ, Stabenfeldt GH, Cragun JR, Addiego LA, Overstreet JW, Lasley BL (1991) Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37(6):838–844PubMedGoogle Scholar
  34. Nelson ME, Fiatarone MA, Morganti CM, Trice I, Greenberg RA, Evans WJ (1994) Effects of high-intensity strength training on multiple risk factors for osteoporotic fractures. A randomized controlled trial. JAMA 272:1909–1914PubMedCrossRefGoogle Scholar
  35. O’Donnell E, Kirwan LD, Goodman JM (2009) Aerobic exercise training in healthy postmenopausal women: effects of hormone therapy. Menopause 16(4):770–776PubMedCrossRefGoogle Scholar
  36. Orsatti FL, Nahas EA, Maesta N, Nahas-Neto J, Burini RC (2008) Plasma hormones, muscle mass and strength in resistance-trained postmenopausal women. Maturitas 59:394–404PubMedCrossRefGoogle Scholar
  37. Panter-Brick C, Lotstein DS, Ellison PT (1993) Seasonality of reproductive function and weight loss in rural Nepali women. Hum Reprod 8:684–690PubMedGoogle Scholar
  38. Pasagian-Macaulay A, Meilahn EN, Bradlow HL, Sepkovic DW, Buhari AM, Simkin-Silverman L, Wing RR, Kuller LH (1996) Urinary markers of estrogen metabolism 2- and 16 alpha-hydroxylation in premenopausal women. Steroids 61:461–467PubMedCrossRefGoogle Scholar
  39. Pasqualini JR (2009) Progestagens in the menopause in healthy women and breast cancer patients. Maturitas 62:343–348PubMedCrossRefGoogle Scholar
  40. Pate RR (1995) Physical activity and health: dose-response issues. Res Q Exerc Sport 66:313–317PubMedGoogle Scholar
  41. Prior JC, Vigna Y, Sciarretta D, Alojado N, Schulzer M (1987) Conditioning exercise decreases premenstrual symptoms: a prospective, controlled 6-month trial. Fertil Steril 47:402–408PubMedGoogle Scholar
  42. Ronkainen H, Pakarinen A, Kirkinen P, Kauppila A (1985) Physical exercise-induced changes and season-associated differences in the pituitary-ovarian function of runners and joggers. J Clin Endocrinol Metab 60:416–422PubMedCrossRefGoogle Scholar
  43. Roussel M, Garnier S, Lemoine S, Gaubert I, Charbonnier L, Auneau G, Mauriège P (2009) Influence of a walking program on the metabolic risk profile of obese postmenopausal women. Menopause 16(3):566–575PubMedCrossRefGoogle Scholar
  44. Santoro N, Brown JR, Adel T, Skurnick JH (1996) Characterization of reproductive hormonal dynamics in the perimenopause. J Clin Endocrinol Metab 81(4):1495–1501PubMedCrossRefGoogle Scholar
  45. Sipila S, Poutamo J (2003) Muscle performance, sex hormones and training in peri-menopausal and post-menopausal women. Scand J Med Sci Sports 13:19–25PubMedCrossRefGoogle Scholar
  46. Slayden SM, Crabbe L, Bae S, Potter HD, Azziz R, Parker CR Jr (1998) The effect of 17 beta-estradiol on adrenocortical sensitivity, responsiveness, and steroidogenesis in postmenopausal women. J Clin Endocrinol Metab 83:519–524PubMedCrossRefGoogle Scholar
  47. Sowers MR, La Pietra M (1995) Menopause: its epidemiology and potential associations with chronic diseases. Epidemiol Rev 17:287–302PubMedGoogle Scholar
  48. Stefanick ML, Mackey S, Sheehan M, Ellsworth N, Haskell WL, Wood PD (1998) Effects of diet and exercise in men and postmenopausal women with low levels of HDL cholesterol and high levels of LDL cholesterol. N Engl J Med 339:12–20PubMedCrossRefGoogle Scholar
  49. Stoddard JL, Dent CW, Shames L, Bernstein L (2007) Exercise training effects on premenstrual distress and ovarian steroid hormones. Eur J Appl Physiol 99:27–37PubMedCrossRefGoogle Scholar
  50. Thieme D, Anielski P, Grosse J, Sachs H, Mueller RK (2003) Identification of anabolic steroids in serum, urine, sweat and hair comparison of metabolic patterns. Anal Chim Acta 483:C299–C306CrossRefGoogle Scholar
  51. Tripp K, Dubois M, Delahaut P, Verstegen J (2009) Detection and identification of plasma progesterone metabolites in the female Florida manatee (Trichechus manatus latirostris) using GC/MS/MS. Theriogenology 72:C365–C371CrossRefGoogle Scholar
  52. Verkasalo PK, Thomas HV, Appleby PN, Davey GK, Key TJ (2001) Circulating levels of sex hormones and their relation to risk factors for breast cancer: a cross-sectional study in 1092 pre- and postmenopausal women (United Kingdom). Cancer Causes Control 12:47–59PubMedCrossRefGoogle Scholar
  53. Warren MP, Perlroth NE (2001) The effects of intense exercise on the female reproductive system. J Endocrinol 170(1):3–11PubMedCrossRefGoogle Scholar
  54. Zarins ZA, Wallis GA, Faghihnia N, Johnson ML, Fattor JA, Horning MA, Brooks GA (2009) Effects of endurance training on cardiorespiratory fitness and substrate partitioning in postmenopausal women. Metabolism 58(9):1338–1346PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • María Concepción Robles Gil
    • 1
    • 5
    Email author
  • R. Timón
    • 1
  • A. F. Toribio
    • 2
  • D. Muñoz
    • 1
  • J. I. Maynar
    • 2
  • M. J. Caballero
    • 3
  • M. Maynar
    • 4
  1. 1.Department of Physical Education and Sport, Sport Sciences FacultyUniversity of ExtremaduraBadajozSpain
  2. 2.Department of Analytical Chemistry, Science FacultyUniversity of ExtremaduraBadajozSpain
  3. 3.Unit of Pharmacology, School of MedicineUniversity of ExtremaduraBadajozSpain
  4. 4.Department of Physiology, Sport Sciences FacultyUniversity of ExtremaduraBadajozSpain
  5. 5.CáceresSpain

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