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European Journal of Applied Physiology

, Volume 111, Issue 10, pp 2571–2580 | Cite as

Effects of sprint training combined with vegetarian or mixed diet on muscle carnosine content and buffering capacity

  • Audrey Baguet
  • Inge Everaert
  • Hélène De Naeyer
  • Harmen Reyngoudt
  • Sanne Stegen
  • Sam Beeckman
  • Eric Achten
  • Lander Vanhee
  • Anneke Volkaert
  • Mirko Petrovic
  • Youri Taes
  • Wim Derave
Original Article

Abstract

Carnosine is an abundant dipeptide in human skeletal muscle with proton buffering capacity. There is controversy as to whether training can increase muscle carnosine and thereby provide a mechanism for increased buffering capacity. This study investigated the effects of 5 weeks sprint training combined with a vegetarian or mixed diet on muscle carnosine, carnosine synthase mRNA expression and muscle buffering capacity. Twenty omnivorous subjects participated in a 5 week sprint training intervention (2–3 times per week). They were randomized into a vegetarian and mixed diet group. Measurements (before and after the intervention period) included carnosine content in soleus, gastrocnemius lateralis and tibialis anterior by proton magnetic resonance spectroscopy (1H-MRS), true-cut biopsy of the gastrocnemius lateralis to determine in vitro non-bicarbonate muscle buffering capacity, carnosine content (HPLC method) and carnosine synthase (CARNS) mRNA expression and 6 × 6 s repeated sprint ability (RSA) test. There was a significant diet × training interaction in soleus carnosine content, which was non-significantly increased (+11%) with mixed diet and non-significantly decreased (−9%) with vegetarian diet. Carnosine content in other muscles and gastrocnemius buffer capacity were not influenced by training. CARNS mRNA expression was independent of training, but decreased significantly in the vegetarian group. The performance during the RSA test improved by training, without difference between groups. We found a positive correlation (r = 0.517; p = 0.002) between an invasive and non-invasive method for muscle carnosine quantification. In conclusion, this study shows that 5 weeks sprint training has no effect on the muscle carnosine content and carnosine synthase mRNA.

Keywords

Muscle carnosine Muscle buffering capacity Sprint training Vegetarian diet 

Notes

Acknowledgments

This study was financially supported by grants from the Research Foundation—Flanders (FWO 1.5.149.08 and G.0046.09) Audrey Baguet is a recipient of a PhD-scholarship from the Research Foundation—Flanders. The practical contribution of Jonathan Dehenau, Job Franssen and Bavo Verhasselt is greatly acknowledged.

The experiments of this manuscript comply with the current laws of Belgium.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Abe H (2000) Role of histidine-related compounds as intracellular proton buffering constituents in vertebrate muscle. Biochemistry (Mosc) 65:757–765Google Scholar
  2. Baguet A, Reyngoudt H, Pottier A, Everaert I, Callens S, Achten E, Derave W (2009) Carnosine loading and washout in human skeletal muscles. J Appl Physiol 106:837–842PubMedCrossRefGoogle Scholar
  3. Baguet A, Bourgois J, Vanhee L, Achten E, Derave W (2010a) Important role of muscle carnosine in rowing performance. J Appl Physiol 109:1096–1101PubMedCrossRefGoogle Scholar
  4. Baguet A, Koppo K, Pottier A, Derave W (2010b) Beta-Alanine supplementation reduces acidosis but not oxygen uptake response during high-intensity cycling exercise. Eur J Appl Physiol 108:495–503PubMedCrossRefGoogle Scholar
  5. Bakardjiev A, Bauer K (1994) Transport of beta-alanine and biosynthesis of carnosine by skeletal muscle cells in primary culture. Eur J Biochem 225:617–623PubMedCrossRefGoogle Scholar
  6. Bell GJ, Wenger HA (1988) The effect of one-legged sprint training on intramuscular pH and nonbicarbonate buffering capacity. Eur J Appl Physiol Occup Physiol 58:158–164PubMedCrossRefGoogle Scholar
  7. Bishop D, Edge J, Goodman C (2004) Muscle buffer capacity and aerobic fitness are associated with repeated-sprint ability in women. Eur J Appl Physiol 92:540–547PubMedCrossRefGoogle Scholar
  8. Bishop DJ, Edge J, Thomas C, Mercier J (2008) Effects of high-intensity training on muscle lactate transporters and post-exercise recovery of muscle lactate and hydrogen ions in women. Am J Physiol Regul Integr Comp Physiol 295(6):R1991–R1998Google Scholar
  9. Bishop D, Edge J, Mendez-Villanueva A, Thomas C, Schneiker K (2009) High-intensity exercise decreases muscle buffer capacity via a decrease in protein buffering in human skeletal muscle. Pflugers Arch 458:929–936PubMedCrossRefGoogle Scholar
  10. Boldyrev A (2007) Carnosine and oxidative stress in cells and tissues. Nova Science Publishers, New YorkGoogle Scholar
  11. Burke DG, Chilibeck PD, Parise G, Candow DG, Mahoney D, Tarnopolsky M (2003) Effect of creatine and weight training on muscle creatine and performance in vegetarians. Med Sci Sports Exerc 35:1946–1955PubMedCrossRefGoogle Scholar
  12. Derave W, Everaert I, Beeckman S, Baguet A (2010) Muscle carnosine metabolism and beta-alanine supplementation in relation to exercise and training. Sports Med 40:247–263PubMedCrossRefGoogle Scholar
  13. Derveaux S, Vandesompele J, Hellemans J (2010) How to do successful gene expression analysis using real-time PCR. Methods 50:227–230PubMedCrossRefGoogle Scholar
  14. Drozak J, Veiga-da-Cunha M, Vertommen D, Stroobant V, Van SE (2010) Molecular identification of carnosine synthase as ATP-grasp domain-containing protein 1 (ATPGD1). J Biol Chem 285:9346–9356PubMedCrossRefGoogle Scholar
  15. Dunnett M, Harris RC (1999) Influence of oral beta-alanine and l-histidine supplementation on the carnosine content of the gluteus medius. Equine Vet J Suppl 30:499–504PubMedGoogle Scholar
  16. Edge J, Bishop D, Goodman C (2006a) The effects of training intensity on muscle buffer capacity in females. Eur J Appl Physiol 96:97–105PubMedCrossRefGoogle Scholar
  17. Edge J, Bishop D, Hill-Haas S, Dawson B, Goodman C (2006b) Comparison of muscle buffer capacity and repeated-sprint ability of untrained, endurance-trained and team-sport athletes. Eur J Appl Physiol 96:225–234CrossRefGoogle Scholar
  18. Edge J, Hill-Haas S, Goodman C, Bishop D (2006c) Effects of resistance training on H+ regulation, buffer capacity, and repeated sprints. Med Sci Sports Exerc 38:2004–2011PubMedCrossRefGoogle Scholar
  19. Everaert I, Mooyaart A, Baguet A, Zutinic A, Baelde H, Achten E, Taes Y, De Heer E, Derave W. 2010. Vegetarianism, female gender and increasing age, but not CNDP1 genotype, are associated with reduced muscle carnosine levels in humans. Amino AcidsGoogle Scholar
  20. Gibala MJ, Little JP, van EM, Wilkin GP, Burgomaster KA, Safdar A, Raha S, Tarnopolsky MA et al (2006) Short-term sprint interval versus traditional endurance training: similar initial adaptations in human skeletal muscle and exercise performance. J Physiol 575:901–911PubMedCrossRefGoogle Scholar
  21. Harmer AR, McKenna MJ, Sutton JR, Snow RJ, Ruell PA, Booth J, Thompson MW, Mackay NA, Stathis CG, Crameri RM, Carey MF, Eager DM (2000) Skeletal muscle metabolic and ionic adaptations during intense exercise following sprint training in humans. J Appl Physiol 89:1793–1803PubMedGoogle Scholar
  22. Harris RC, Marlin DJ, Dunnett M, Snow DH, Hultman E (1990) Muscle buffering capacity and dipeptide content in the thoroughbred horse, greyhound dog and man. Comp Biochem Physiol A Comp Physiol 97:249–251PubMedCrossRefGoogle Scholar
  23. Harris RC, Jones G, Hill C, Kendrick IP, Boobis L, Kim CK, Kim HJ, Dang VH, Edge J, Wise JA (2007) The carnosine content in V Lateralis of vegetarians and omnivores (abstract). FASEB J 6:A944Google Scholar
  24. Hill CA, Harris RC, Kim HJ, Harris BD, Sale C, Boobis LH, Kim CK, Wise JA (2007) Influence of beta-alanine supplementation on skeletal muscle carnosine concentrations and high-intensity cycling capacity. Amino Acids 32:225–233PubMedCrossRefGoogle Scholar
  25. Hirakoba K (1999) Buffering capacity in human skeletal muscle: a brief review. Bull Faculty Compt Sci Syst Eng Kyushu Inst Technol (Hum Sci) 12:1–21Google Scholar
  26. Hultman E, Sahlin K (1980) Acid–base balance during exercise. Exerc Sport Sci Rev 8:41–128PubMedGoogle Scholar
  27. Iaia FM, Thomassen M, Kolding H, Gunnarsson T, Wendell J, Rostgaard T, Nordsborg N, Krustrup P, Nybo L, Hellsten Y, Bangsbo J (2008) Reduced volume but increased training intensity elevates muscle Na+–K+ pump alpha1-subunit and NHE1 expression as well as short-term work capacity in humans. Am J Physiol Regul Integr Comp Physiol 294:R966–R974PubMedCrossRefGoogle Scholar
  28. Juel C (1998) Muscle pH regulation: role of training. Acta Physiol Scand 162:359–366PubMedCrossRefGoogle Scholar
  29. Juel C, Halestrap AP (1999) Lactate transport in skeletal muscle—role and regulation of the monocarboxylate transporter. J Physiol 517(Pt 3):633–642PubMedCrossRefGoogle Scholar
  30. Juel C, Klarskov C, Nielsen JJ, Krustrup P, Mohr M, Bangsbo J (2004) Effect of high-intensity intermittent training on lactate and H + release from human skeletal muscle. Am J Physiol Endocrinol Metab 286:E245–E251PubMedCrossRefGoogle Scholar
  31. Kendrick IP, Harris RC, Kim HJ, Kim CK, Dang VH, Lam TQ, Bui TT, Smith M, Wise JA (2008) The effects of 10 weeks of resistance training combined with beta-alanine supplementation on whole body strength, force production, muscular endurance and body composition. Amino Acids 34(4):547–554PubMedCrossRefGoogle Scholar
  32. Kendrick IP, Kim HJ, Harris RC, Kim CK, Dang VH, Lam TQ, Bui TT, Wise JA (2009) The effect of 4 weeks beta-alanine supplementation and isokinetic training on carnosine concentrations in type I and II human skeletal muscle fibres. Eur J Appl Physiol 106:131–138PubMedCrossRefGoogle Scholar
  33. Lukaszuk JM, Robertson RJ, Arch JE, Moore GE, Yaw KM, Kelley DE, Rubin JT, Moyna NM (2002) Effect of creatine supplementation and a lacto-ovo-vegetarian diet on muscle creatine concentration. Int J Sport Nutr Exerc Metab 12:336–348PubMedGoogle Scholar
  34. Mannion AF, Jakeman PM, Dunnett M, Harris RC, Willan PL (1992) Carnosine and anserine concentrations in the quadriceps femoris muscle of healthy humans. Eur J Appl Physiol Occup Physiol 64:47–50PubMedCrossRefGoogle Scholar
  35. Mannion AF, Jakeman PM, Willan PL (1994) Effects of isokinetic training of the knee extensors on high-intensity exercise performance and skeletal muscle buffering. Eur J Appl Physiol Occup Physiol 68:356–361PubMedCrossRefGoogle Scholar
  36. McMahon S, Wenger HA (1998) The relationship between aerobic fitness and both power output and subsequent recovery during maximal intermittent exercise. J Sci Med Sport 1:219–227PubMedCrossRefGoogle Scholar
  37. Mohr M, Krustrup P, Nielsen JJ, Nybo L, Rasmussen MK, Juel C, Bangsbo J (2007) Effect of two different intense training regimens on skeletal muscle ion transport proteins and fatigue development. Am J Physiol Regul Integr Comp Physiol 292:R1594–R1602PubMedCrossRefGoogle Scholar
  38. Nevill ME, Boobis LH, Brooks S, Williams C (1989) Effect of training on muscle metabolism during treadmill sprinting. J Appl Physiol 67:2376–2382PubMedGoogle Scholar
  39. Parkhouse WS, McKenzie DC (1984) Possible contribution of skeletal muscle buffers to enhanced anaerobic performance: a brief review. Med Sci Sports Exerc 16:328–338PubMedGoogle Scholar
  40. Parkhouse WS, McKenzie DC, Hochachka PW, Ovalle WK (1985) Buffering capacity of deproteinized human vastus lateralis muscle. J Appl Physiol 58:14–17PubMedGoogle Scholar
  41. Pilegaard H, Domino K, Noland T, Juel C, Hellsten Y, Halestrap AP, Bangsbo J (1999) Effect of high-intensity exercise training on lactate/H + transport capacity in human skeletal muscle. Am J Physiol 276:E255–E261PubMedGoogle Scholar
  42. Ramadan S, Ratai EM, Wald LL, Mountford CE (2010) In vivo 1D and 2D correlation MR spectroscopy of the soleus muscle at 7T. J Magn Reson 204:91–98PubMedCrossRefGoogle Scholar
  43. Ross A, Leveritt M, Riek S (2001) Neural influences on sprint running: training adaptations and acute responses. Sports Med 31:409–425PubMedCrossRefGoogle Scholar
  44. Sahlin K, Henriksson J (1984) Buffer capacity and lactate accumulation in skeletal muscle of trained and untrained men. Acta Physiol Scand 122:331–339PubMedCrossRefGoogle Scholar
  45. Sanchez-Hernandez L, Marina ML, Crego AL (2011) A capillary electrophoresis-tandem mass spectrometry methodology for the determination of non-protein amino acids in vegetable oils as novel markers for the detection of adulterations in olive oils. J Chromatogr (in Press)Google Scholar
  46. Sharp R, Costill D, Fink W, King D (1986) Effects of eight weeks of bicycle ergometer sprint training on human muscle buffering capacity. Int J Sports Med 7:13–17PubMedCrossRefGoogle Scholar
  47. Suzuki Y, Ito O, Takahashi H, Takamatsu K (2004) The effect of sprint training on skeletal muscle carnosine in humans. Int J Sport Health Sci 2:105–110CrossRefGoogle Scholar
  48. Thomas C, Bishop D, Moore-Morris T, Mercier J (2007) Effects of high-intensity training on MCT1, MCT4, and NBC expressions in rat skeletal muscles: influence of chronic metabolic alkalosis. Am J Physiol Endocrinol Metab 293:E916–E922PubMedCrossRefGoogle Scholar
  49. Troup JP, Metzger JM, Fitts RH (1986) Effect of high-intensity exercise training on functional capacity of limb skeletal muscle. J Appl Physiol 60:1743–1751PubMedGoogle Scholar
  50. Venderley AM, Campbell WW (2006) Vegetarian diets : nutritional considerations for athletes. Sports Med 36:293–305PubMedCrossRefGoogle Scholar
  51. Weston AR, Myburgh KH, Lindsay FH, Dennis SC, Noakes TD, Hawley JA (1997) Skeletal muscle buffering capacity and endurance performance after high-intensity interval training by well-trained cyclists. Eur J Appl Physiol Occup Physiol 75:7–13PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Audrey Baguet
    • 1
  • Inge Everaert
    • 1
  • Hélène De Naeyer
    • 1
  • Harmen Reyngoudt
    • 2
  • Sanne Stegen
    • 1
  • Sam Beeckman
    • 1
  • Eric Achten
    • 2
  • Lander Vanhee
    • 1
  • Anneke Volkaert
    • 1
  • Mirko Petrovic
    • 3
  • Youri Taes
    • 4
  • Wim Derave
    • 1
  1. 1.Department of Movement and Sports SciencesGhent UniversityGhentBelgium
  2. 2.Department of Radiology and Nuclear Medicine, Ghent Institute for functional and Metabolic Imaging (GIfMI)Ghent UniversityGhentBelgium
  3. 3.Department of Internal MedicineGhent UniversityGhentBelgium
  4. 4.Department of EndocrinologyGhent University HospitalGhentBelgium

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