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European Journal of Applied Physiology

, Volume 111, Issue 8, pp 1829–1839 | Cite as

Effects of 6 weeks of n-3 fatty acids and antioxidant mixture on lipid peroxidation at rest and postexercise

  • E. Filaire
  • A. Massart
  • M. Rouveix
  • Hugues Portier
  • F. Rosado
  • D. Durand
Original Article

Abstract

The purpose of this randomized study was to measure the influence of 6 weeks of LCPUFA (600 mg EPA and 400 mg DHA per day) supplementation alone or in association with 30 mg vitamin E, 60 mg vitamin C and 6 mg β-carotene on resting and exercise-induced lipid peroxidation in judoists (n = 36). Blood samples were collected at rest before (T 1) and after the supplementation period, in preexercise (T 2) and postexercise (T 3) conditions, for analysis of α-tocopherol, retinol, lag phase (Lp) before free radical-induced oxidation, maximum rate of oxidation (R max) during the propagating chain reaction, maximum amount of conjugated dienes (CDmax) accumulated after the propagation phase, and nitric oxide, malondialdehyde and lipoperoxide (POOL) concentrations. Dietary data were collected using a 7-day diet record. There were no significant differences among treatment groups with respect to habitual intakes of energy from fat, carbohydrate, or protein. At T 1, there were no significant differences among treatment groups with respect to lipid peroxidation, lag phase, and levels of α-tocopherol or retinol. The consumption of an n-3 LC PUFA supplement increased oxidative stress at rest and did not attenuate the exercise-induced oxidative stress. The addition of antioxidants did not prevent the formation of oxidation products at rest. On the contrary, it seems that the combination of antioxidants added to the n-3 LCPUFA supplement led to a decrease in, CDmax, R max, and POOL and MDA concentrations after a judo training session.

Keywords

Judo Omega-3 Supplementation Vitamin Oxidative stress 

References

  1. Agarwal R, Chase SD (2002) Rapid, fluorimetric-liquid chromatographic determination of malondialdehyde in biological samples. J Chromatogr 775:121–126CrossRefGoogle Scholar
  2. Allard J, Kurian R, Aghdassi E, Muggli R, Royall D (1997) Lipid peroxidation during n-3 fatty acid and vitamin E supplementation in humans. Lipids 32:535–541PubMedCrossRefGoogle Scholar
  3. Altan O, Pabuccuoglu A, Altan A, Konyalioglu S, Bayraktar H (2003) Effect of heat stress on oxidative stress, lipid peroxidation and some stress parameters in broilers. Br Poult Sci 44:545–550PubMedCrossRefGoogle Scholar
  4. Andrade PMM, Ribeiro BG, Bozza MT, Costa Rosa LFB, Tavares do Carmo MG (2007) Effects of the fish-oil supplementation on the immune and inflammatory responses in elite swimmers. Prost Leukot Essent Fat Acids 77:139–145CrossRefGoogle Scholar
  5. Armstrong RB, Ogilvie RW, Schwane JA (1983) Eccentric exercise-induced injury to rat skeletal muscle. J Appl Physiol 54:80–93PubMedGoogle Scholar
  6. Bauchart D, Gobert M, Habeanu M, Parafita E, Gruffat D, Durand D (2010) Influence des acides gras polyinsaturés n-3 et des antioxydants alimentaires sur les acides gras de la viande et la lipoperoxydation chez le bovin en finition. Oléagineux, Corps gras, Lipides 17:30–36Google Scholar
  7. Bloomer RJ, Goldfarb AH (2004) Anaerobic exercise and oxidative stress: a review. Can J Appl Physiol 29:245–263Google Scholar
  8. Bloomer RJ, Goldfarb AH, McKenzie MJ (2006) Oxidative stress response to aerobic exercise: comparison of antioxidant supplements. Med Sci Sports Exerc 38:1098–1105PubMedCrossRefGoogle Scholar
  9. Bloomer RJ, Larson DE, Fisher-Wellman KH, Galpin AJ, Schilling BK (2009) Effect of eicosapentaenoic and docosahexaenoic acid on resting and exercise-induced inflammatory and oxidative stress biomarkers: a randomized, placebo controlled, cross-over study. Lipids Health Dis 19:8–36Google Scholar
  10. Bryan NS, Grisham MB (2007) Methods to detect nitric oxide and its metabolites in biological samples. Free Rad Biol Med 43:645–657PubMedCrossRefGoogle Scholar
  11. Cholewa J, Poprzecki S, Zajac A, Waskiewicz Z (2008) The influence of vitamin C on blood oxidative stress parameters in basketball players in response to maximal exercise. Sci Sports 23:176–182CrossRefGoogle Scholar
  12. Clarkson PM, Thompson HS (2000) Antioxidants: what role do they play in physical activity and health? Am J Clin Nutr 72:637S–646SPubMedGoogle Scholar
  13. Cohen J (1988) Statistical analysis for the behavioural sciences. Lawrence Erlbaum, HillsdaleGoogle Scholar
  14. Davison G, Gleeson M, Phillips S (2007) Antioxidant supplementation and immunoendocrine responses to prolonged exercise. Med Sci Sports Exerc 39:645–652PubMedCrossRefGoogle Scholar
  15. Degoutte F, Jouanel P, Filaire E (2003) Energy demands during a judo match and recovery. Br J Sports Med 37:245–249PubMedCrossRefGoogle Scholar
  16. Diplock AT, Rice-Evans AC, Burton RH (1994) Is there a significant role for lipid peroxidation in the causation of malignancy and for antioxidants in cancer prevention? Cancer Res 54:19525–19526Google Scholar
  17. Durnin JVGA, Rahaman MM (1967) The assessment of the amount of fat in the human body from measurement of skinfold thickness. Br J Nutr 21:681–689PubMedCrossRefGoogle Scholar
  18. Esterbauer H, Striegl G, Puhl H, Rotheneder M (1989) Continuous monitoring of in vitro oxidation of human low density lipoprotein. Free Rad Res Comm 6:67–75CrossRefGoogle Scholar
  19. Finaud J, Degoutte F, Scislowski V, Rouveix M, Durand D, Filaire E (2006) Competition and food restriction effects on oxidative stress in judo. Int J Sports Med 27:834–841PubMedCrossRefGoogle Scholar
  20. Fisher-Wellman K, Bloomer RJ (2009) Acute exercise and oxidative stress: a 30 year history. Dyn Med 13:1–25CrossRefGoogle Scholar
  21. Gladine C, Morand C, Rock E, Bauchart D, Durand D (2007) Plantation extracts rich in polyphenols are efficient antioxidants to prevent lipoperoxidation in plasma lipids from animals fed PUFA n-3 supplemented diets. Anim Feed Sci Technol 136:281–296CrossRefGoogle Scholar
  22. Gobert M, Martin B, Ferlay A, Chilliard Y, Graulet B, Pradel P, Bauchart D, Durand D (2009) Plant polyphenols associated with vitamin E can reduce plasma lipoperoxidation in dairy cows given n-3 polyunsaturated fatty acids. J Dairy Sci 92:6095–6104PubMedCrossRefGoogle Scholar
  23. Goldfarb AH, Bloomer RJ, McKenzie MJ (2005) Combined antioxidant treatment effects on blood oxidative stress after eccentric exercise. Med Sci Sports Exerc 37:234–239PubMedCrossRefGoogle Scholar
  24. Gomez-Cabrera MC, Domenech E, Vina J (2008) Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Rad Biol Med 44:126–131PubMedCrossRefGoogle Scholar
  25. Groussard R-B, Machefer C, Vincent S, Cillard G-D (2003) Changes in blood lipid peroxidation markers and antioxidants after a single sprint anaerobic exercise. Eur J Appl Physiol 89:14–20PubMedCrossRefGoogle Scholar
  26. Guichardant M, Bacot S, Moliere P, Lagarde M (2006) Hydroxy-alkenals from the peroxidation of n-3 and n-6 fatty acids and urinary metabolites. Prostaglandins Leukot Essent Fatty Acids 75:179–182PubMedCrossRefGoogle Scholar
  27. Gutierrez J, Ballinger S, Darley-Usmar VM, Landar A (2006) Free radicals, mitochondria, and oxidized lipids, the emerging role in signal transduction in vascular cells. Circ Res 99:924–932PubMedCrossRefGoogle Scholar
  28. Hale T, Armstrong N, Hardman A, Jakeman P, Sharp C, Winter E (1988) Position statement on the physiological assessment of the elite competitor, 2nd edn. British Association of Sports Sciences, LeedsGoogle Scholar
  29. Hildebrandt W, Alexander S, Bartsch P, Droge W (2002) Effect of N-acetyl-cysteine on the hypoxic ventilatory response and erythropoietin production: linkage between plasma thiol redox state and O2 chemosensitivity. Blood 99:1552–1555PubMedCrossRefGoogle Scholar
  30. Ihara H, Shino Y, Morita Y, Kawaguchi E, Hashizume N, Yoshida M (2001) Is skeletal muscle damaged by the oxidative stress following anaerobic exercise? J Clin Lab Anal 15:239–243PubMedCrossRefGoogle Scholar
  31. Jackson MJ (1999) Free radicals in skin and muscle: damaging agents or signals for adaptation? Proc Nutr Soc 58:673–676PubMedCrossRefGoogle Scholar
  32. Ji Q, Zhang L, Jia H, Xu J (2004) Pentoxifylline inhibits endotoxin-induced NF-kappa B activation and associated production of proinflammatory cytokines. Ann Clin Lab Sci 34:427–436PubMedGoogle Scholar
  33. Karvonen J, Vuorimaa T (1988) Heart rate and exercise intensity during sport activities. Practical application. Sports Med 5:303–312Google Scholar
  34. Keong CC, Singh HJ, Singh R (2006) Effects of palm vitamin E supplementation on exercise-induced oxidative stress and endurance performance in the heat. J Sports Sci Med 5:629–639Google Scholar
  35. Kew S, Banerjee T, Minihane AM, Finnegan YE, Muggli R, Albers R, Williams CM, Calder PC (2003) Lack of effect of foods enriched with plant- or marine-derived n-3 fatty acids on human immune function. Am J Clin Nutr 79:674–681Google Scholar
  36. Khassaf M, McArdle A, Esanu C, Vasilaki A, McArdle F, Griffiths RD, Brodie DA, Jackson MJ (2003) Effect of vitamin C supplements on antioxidant defence and stress proteins in human lymphocytes and skeletal muscle. J Physiol 549:645–652PubMedCrossRefGoogle Scholar
  37. Kraemer WJ, Fry AC, Rubin MR, Triplett-McBride T, Gordon SE, Koziris LP, Lynch JM, Volek JS, Meuffels DE, Newton RU, Fleck SJ (2001) Physiological and performance responses to tournament wrestling. Med Sci Sport Exerc 33:1367–1368CrossRefGoogle Scholar
  38. Lamprecht M, Hofman P, Greilberger JF, Schwaberger G (2009) Increased lipid peroxidation in trained men after 2 weeks of antioxidant supplementation. Int J Sport Nutr Exerc Metab 19:385–399PubMedGoogle Scholar
  39. Lepage G, Munoz G, Champagne J, Roy CC (1991) Preparative steps necessary for the accurate measurement of malondialdehyde by high-performance liquid chromatography. Anal Biochem 197:277–283PubMedCrossRefGoogle Scholar
  40. Mastaloudis A, Morrow JD, Hopkins DW, Devaraj S, Traber MG (2004) Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners. Free Radic Biol Med 36:1329–1341PubMedCrossRefGoogle Scholar
  41. McAnulty SR, MacAnulty LS, Nieman DC, Morrow JD, Shooter LA, Holmes S, Heward C, Henson DA (2005) Effect of alpha-tocopherol supplementation on plasma homocysteine and oxidative stress in highly trained athletes before and after exhaustive exercise. J Nutr Biochem 16:530–537PubMedCrossRefGoogle Scholar
  42. McAnulty SR, Nieman DC, Fox-Rabinovich M, Duran V, McAnulty LS, Henson DA, Jin F, Landram MJ (2010) Effect of n-3 fatty acids and antioxidants on oxidative stress after exercise. Med Sci Sports Exerc 12:1704–1711Google Scholar
  43. Miret S, Saiz MP, Mitjavila MT (2003) Effect of fish oil- and olive oil-rich diets on iron metabolism and oxidative stress in rat. Br J Nutr 89:11–18PubMedCrossRefGoogle Scholar
  44. Mori TA, Puddey IB, Burke V, Croft KD, Dunstan DW, Rivera JH, Beilin LJ (2000) Effect of omega 3 fatty acids on oxidative stress in humans: GC-MS measurement of urinary F2-isoprostane excretion. Redox Rep 5:45–46PubMedGoogle Scholar
  45. Morrow JD, Roberts LJ (1997) The isoprostanes: unique bioactive products of lipid peroxidation. Prog Lipid Res 36:1–21PubMedCrossRefGoogle Scholar
  46. Mukai K, Sawada K, Kohno Y, Terao J (1993) Kinetic study of the prooxidant effect of tocopherol. Hydrogen abstraction from lipid hydroperoxides by tocopheroxyls in solution. Lipids 28:747–752CrossRefGoogle Scholar
  47. Nieman DC, Henson DA, McAnulty SR, McAnulty L, Swick NS, Utter AC, Vinci DM, Opiela SJ, Morrow JD (2002) Influence of vitamin C supplementation on oxidative and immune changes after an ultramarathon. J Appl Physiol 92:1970–1977PubMedGoogle Scholar
  48. Nieman DC, Henson DA, McAnulty SR, Jin F, Maxwell KR (2009) n-3 polyunsaturated fatty acids do not alter immune and inflammation measures in endurance athletes. Int J Sport Nutr Exerc Metab 19:536–546PubMedGoogle Scholar
  49. Niki E (2009) Lipid peroxidation: physiological levels and dual biological effects. Free Rad Biol Med 47:469–484PubMedCrossRefGoogle Scholar
  50. Oostenbrug GS, Mensink RP, Hardeman MR, De Vries T, Brouns F, Hornstra G (1997) Exercise performance, red blood cell deformability, and lipid peroxidation: effects of fish oil and vitamin E. J Appl Physiol 83:746–752PubMedGoogle Scholar
  51. Palazzetti S, Richard MJ, Favier A, Margaritis I (2003) Overload training increases exercise-induced oxidative stress and damage. Can J Appl Physiol 28:559–565CrossRefGoogle Scholar
  52. Peres G (2000) Physiologie de l’exercice musculaire et nutrition du sportif. In: Brunet-Guedj E, Comtet B, Genety J (eds) Abrégé de médecine du sport. Masson, ParisGoogle Scholar
  53. Poprzecki S, Zajac A, Chalimoniuk M, Waskiewicz Z, Langfort J (2009) Modification of blood antioxidant status and lipid profile in response to high-intensity endurance exercise after low doses of omega-3 polyunsaturated fatty acids supplementation in healthy volunteers. Int J Food Sci Nutr 60:67–79PubMedCrossRefGoogle Scholar
  54. Roberts LJ, Oates JA, Linton MF, Fazio S, Meador BP, Gross MD, Shyr Y, Morrow JD (2007) The relationship between dose of vitamin E and suppression of oxidative stress in humans. Free Radic Biol Med 43:1388–1393PubMedCrossRefGoogle Scholar
  55. Rohrbach S (2009) Effects of dietary polyunsaturated fatty acids on mitochondria. Curr Pharm Des 15:1–14CrossRefGoogle Scholar
  56. Sacheck JM, Blumberg JB (2001) Role of vitamin E and oxidative stress in exercise. Nutrition 17:809–814PubMedCrossRefGoogle Scholar
  57. Schmidt EB, Arnesen H, Caterinade R, Rasmussen LH, Kristensen SD (2005) Marine n-3 polyunsaturated fatty acid and coronary disease. Thromb Res 115:163–170PubMedCrossRefGoogle Scholar
  58. Schnitzer E, Pinchuk I, Fainaru M, Schafer Z, Lichtenberg D (1995) Copper-induced lipid oxidation in unfractionated plasma: the lag preceding oxidation as a measure of oxidation-resistance. Biochem Biophys Res Comm 216:854–861PubMedCrossRefGoogle Scholar
  59. Sijben JW, Calder PC (2007) Differential immunomodulation with long-chain n-3 PUFA in health and chronic disease. Proc Nutr Soc 66:237–259PubMedCrossRefGoogle Scholar
  60. Sikorski W, Mickiewicz B, Maole C (1987) Structure of the contest and work capacity of the judoist. Polish Judo Association Institute of Sports, WarsawGoogle Scholar
  61. Tang JL, Faustman C, Hoagland TA, Mancini RA, Seyfert M, Hunt MC (2005) Interactions between mitochondrial lipid oxidation and oxymyoglobin oxidation and the effects of vitamin E. J Agric Food Chem 53:6073–6079PubMedCrossRefGoogle Scholar
  62. Teixeira VH, Valente HF, Casal SI, Marques AF, Moreira PA (2009a) Antioxidant status, oxidative stress, and damage in elite trained kayakers and canoeists and sedentary controls. Int J Sport Nutr Exerc Metab 19:443–456PubMedGoogle Scholar
  63. Teixeira VH, Valente HF, Casal SI, Marques AF, Moreira PA (2009b) Antioxidants do not prevent postexercise peroxidation and may delay muscle recovery. Med Sci Sports Exerc 41:1752–1760PubMedCrossRefGoogle Scholar
  64. Van Beaumont W, Greeland JE, Juhos L (1972) Disproportional changes in hematocrit, plasma volume and proteins during exercise and best rest. J Appl Physiol 33:55–61PubMedGoogle Scholar
  65. Venkatraman JT, Angkeow P, Satsangi N, Fernandes G (1998) Effects of dietary n-6 and n-3 lipids on antioxidant defense system in livers of exercised rats. J Am Coll Nutr 17(6):586–594Google Scholar
  66. Vina J, Gomez-Cabrera MC, Lloret A, Marquez R, Minana JB, Pallardo FV, Sastre J (2000) Free radicals in exhaustive physical exercise: mechanism of production, and protection by antioxidants. IUBMB Life 50:271–277PubMedCrossRefGoogle Scholar
  67. Williams CA, Carlucci SA (2006) Oral vitamin E supplementation on oxidative stress, vitamin and antioxidant status in intensely exercised horses. Equine Vet J 36:617–621CrossRefGoogle Scholar
  68. Zhang P, Omaye ST (2001) DNA, strand breakage and oxygen tension: effects of beta-carotene, alpha-tocopherol, and ascorbic acid. Food Chem Toxicol 39:239–246PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • E. Filaire
    • 1
  • A. Massart
    • 1
  • M. Rouveix
    • 1
  • Hugues Portier
    • 1
  • F. Rosado
    • 3
  • D. Durand
    • 2
  1. 1.Laboratoire CTI Inserm 658UFRSTAPS, 2 allée du ChâteauOrléans CedexFrance
  2. 2.INRA, UR1213 HerbivoresCentre de Clermont-FerrandSt-Genès-ChampanelleFrance
  3. 3.Centro de Estudos BiocineticosFaculdade de Ciencas do Desporto e Educaçao FisicaCoimbraPortugal

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