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SIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: influence of glucose ingestion

Abstract

This study was designed to examine potential in vivo mechanisms of AMP-activated protein kinase (AMPK) phosphorylation inhibition and its downstream signaling consequences during the recovery period after a single bout of sprint exercise. Sprint exercise induces Thr172-AMPK phosphorylation and increased PGC-1α mRNA, by an unknown mechanism. Muscle biopsies were obtained in 15 young healthy men in response to a 30-s sprint exercise (Wingate test) randomly distributed into two groups: the fasting (n = 7, C) and the glucose group (n = 8, G), who ingested 75 g of glucose 1 h before exercising to inhibit AMPKα phosphorylation. Exercise elicited different patterns of Ser221-ACCβ, Ser473-Akt and Thr642-AS160 phosphorylation, during the recovery period after glucose ingestion. Thirty minutes after the control sprint, Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation was reduced by 33% coinciding with increased Thr172-AMPKα phosphorylation (both, P < 0.05). Glucose abolished the 30-min Thr172-AMPKα phosphorylation. Ser221-ACCβ phosphorylation was elevated immediately following and 30 min after exercise in C and G, implying a dissociation between Thr172-AMPKα and Ser221-ACCβ phosphorylation. Two hours after the sprint, PGC-1α protein expression remained unchanged while SIRT1 (its upstream deacetylase) was increased. Glucose ingestion abolished the SIRT1 response without any significant effect on PGC-1α protein expression. In conclusion, glucose ingestion prior to a sprint exercise profoundly affects Thr172-AMPKα phosphorylation and its downstream signaling during the recovery period.

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Acknowledgments

This study was supported by grants from the Ministerio de Educación y Ciencia (BFI2003-09638, BFU2006-13784 and FEDER) and the Gobierno de Canarias (PI2005/177) and FUNCIS (PI/10/07). Borja Guerra is a fellow of the “Recursos Humanos y Difusión de la Investigación” Programe (ISCIII, MSC, Spain). Special thanks are given to José Navarro de Tuero and Oscar Bernales for his excellent technical assistance. The specialized advice from Tony Webster in editing the English version of the manuscript is also acknowledged. Special thanks are given to all subjects who volunteered for these experiments.

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Correspondence to José A. L. Calbet.

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Communicated by Susan Ward.

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Guerra, B., Guadalupe-Grau, A., Fuentes, T. et al. SIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: influence of glucose ingestion. Eur J Appl Physiol 109, 731–743 (2010). https://doi.org/10.1007/s00421-010-1413-y

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Keywords

  • Signaling
  • Wingate
  • Human
  • Skeletal muscle
  • Fatigue
  • Performance