European Journal of Applied Physiology

, Volume 109, Issue 2, pp 343–353 | Cite as

Effects of resistance exercise and protein ingestion on blood leukocytes and platelets in young and older men

  • Juha J. Hulmi
  • T. Myllymäki
  • M. Tenhumäki
  • N. Mutanen
  • R. Puurtinen
  • G. Paulsen
  • A. A. Mero
Original Article

Abstract

This study investigated, in a multi-experiment design, the acute effects of milk protein ingestion, aging [50 young (~26 years) vs. 45 older (~61 years) men] and training state for the blood leukocyte and platelet responses acutely after a single bout of resistance exercise (RE). Moreover, basal effects of 21 weeks of resistance training (RT) were examined. The single bout of RE rapidly increased all blood leukocytes and platelets (P < 0.05). Protein ingestion before or before and after the RE bout did not have an effect on this response. However, younger men had a larger immediate exercise-induced response in leukocytes and platelets than older men. Basal fasting levels of leukocytes and platelets remained unchanged after 21 weeks of RT and this RT period did not change the acute RE-induced leukocyte and platelet response. The long-term RT was, however, able to slightly increase blood hematocrit. Blood platelet counts were consistently higher in the younger men when compared to the older men. Blood lymphopenia occurred only after a larger volume of exercise. In conclusion, the acute increase in blood leukocytes and platelets may be smaller in the older as when compared to the younger men. However, the number of immune cells and thus probably their function may not be affected by milk protein ingestion or months of resistance training.

Keywords

Blood leukocytes Blood platelets Resistance exercise Protein nutrition Immune system Aging Hematocrit 

Notes

Acknowledgments

The authors thank Tuovi Nykänen and Aila Ollikainen for their help in data collection and analysis. We also thank the subjects and research assistants. The Finnish Ministry of Education and the Finnish Cultural Foundation supported this research.

References

  1. Ahmadizad S, El-Sayed MS (2003) The effects of graded resistance exercise on platelet aggregation and activation. Med Sci Sports Exerc 35:1026–1032CrossRefPubMedGoogle Scholar
  2. Ahmadizad S, El-Sayed MS, Maclaren DP (2006) Responses of platelet activation and function to a single bout of resistance exercise and recovery. Clin Hemorheol Microcirc 35:159–168PubMedGoogle Scholar
  3. Alonso-Fernandez P, Puerto M, Mate I, Ribera JM, de la Fuente M (2008) Neutrophils of centenarians show function levels similar to those of young adults. J Am Geriatr Soc 56:2244–2251CrossRefPubMedGoogle Scholar
  4. American College of Sports Medicine (2009) American college of sports medicine position stand. Progression models in resistance training for healthy adults. Med Sci Sports Exerc 41:687–708CrossRefGoogle Scholar
  5. Bobeuf F, Labonte M, Khalil A, Dionne IJ (2009) Effect of resistance training on hematological blood markers in older men and women: a pilot study. Curr Gerontol Geriatr Res 156820 Google Scholar
  6. Cannon JG, Orencole SF, Fielding RA, Meydani M, Meydani SN, Fiatarone MA, Blumberg JB, Evans WJ (1990) Acute phase response in exercise: interaction of age and vitamin E on neutrophils and muscle enzyme release. Am J Physiol 259:R1214–R1219PubMedGoogle Scholar
  7. Cannon JG, Fiatarone MA, Fielding RA, Evans WJ (1994) Aging and stress-induced changes in complement activation and neutrophil mobilization. J Appl Physiol 76:2616–2620PubMedGoogle Scholar
  8. Chamberlain KG, Tong M, Penington DG (1990) Properties of the exchangeable splenic platelets released into the circulation during exercise-induced thrombocytosis. Am J Hematol 34:161–168CrossRefPubMedGoogle Scholar
  9. Convertino VA, Brock PJ, Keil LC, Bernauer EM, Greenleaf JE (1980) Exercise training-induced hypervolemia: role of plasma albumin, renin, and vasopressin. J Appl Physiol 48:665–669PubMedGoogle Scholar
  10. Corberand J, Ngyen F, Laharrague P, Fontanilles AM, Gleyzes B, Gyrard E, Senegas C (1981) Polymorphonuclear functions and aging in humans. J Am Geriatr Soc 29:391–397PubMedGoogle Scholar
  11. Cribb PJ, Williams AD, Stathis CG, Carey MF, Hayes A (2007) Effects of whey isolate, creatine, and resistance training on muscle hypertrophy. Med Sci Sports Exerc 39:298–307CrossRefPubMedGoogle Scholar
  12. Cury-Boaventura MF, Levada-Pires AC, Folador A, Gorjao R, Alba-Loureiro TC, Hirabara SM, Peres FP, Silva PR, Curi R, Pithon-Curi TC (2008) Effects of exercise on leukocyte death: prevention by hydrolyzed whey protein enriched with glutamine dipeptide. Eur J Appl Physiol 103:289–294CrossRefPubMedGoogle Scholar
  13. Durnin JV, Womersley J (1974) Body fat assessed from total body density and its estimation from skinfold thickness: measurements on 481 men and women aged from 16 to 72 years. Br J Nutr 32:77–97CrossRefPubMedGoogle Scholar
  14. Gabriel H, Kindermann W (1997) The acute immune response to exercise: What does it mean? Int J Sports Med 18(suppl 1):S28–S45CrossRefPubMedGoogle Scholar
  15. Galvao DA, Nosaka K, Taaffe DR, Peake J, Spry N, Suzuki K, Yamaya K, McGuigan MR, Kristjanson LJ, Newton RU (2008) Endocrine and immune responses to resistance training in prostate cancer patients. Prostate Cancer Prostatic Dis 11:160–165CrossRefPubMedGoogle Scholar
  16. Gielen S, Schuler G, Hambrecht R (2001) Exercise training in coronary artery disease and coronary vasomotion. Circulation 103:E1–E6PubMedGoogle Scholar
  17. Ha E, Zemel MB (2003) Functional properties of whey, whey components, and essential amino acids: mechanisms underlying health benefits for active people (review). J Nutr Biochem 14:251–258CrossRefPubMedGoogle Scholar
  18. Häkkinen K, Pakarinen A, Kraemer WJ, Häkkinen A, Valkeinen H, Alen M (2001) Selective muscle hypertrophy, changes in EMG and force, and serum hormones during strength training in older women. J Appl Physiol 91:569–580PubMedGoogle Scholar
  19. Hartman JW, Tang JE, Wilkinson SB, Tarnopolsky MA, Lawrence RL, Fullerton AV, Phillips SM (2007) Consumption of fat-free fluid milk after resistance exercise promotes greater lean mass accretion than does consumption of soy or carbohydrate in young, novice, male weightlifters. Am J Clin Nutr 86:373–381PubMedGoogle Scholar
  20. Hu M, Finni T, Sedliak M, Zhou W, Alen M, Cheng S (2008) Seasonal variation of red blood cell variables in physically inactive men: effects of strength training. Int J Sports Med 29:564–568CrossRefPubMedGoogle Scholar
  21. Hulmi JJ, Volek JS, Selänne H, Mero AA (2005) Protein ingestion prior to strength exercise affects blood hormones and metabolism. Med Sci Sports Exerc 37:1990–1997CrossRefPubMedGoogle Scholar
  22. Hulmi JJ, Ahtiainen JP, Kaasalainen T, Pöllänen E, Häkkinen K, Alen M, Selänne H, Kovanen V, Mero AA (2007) Postexercise myostatin and activin IIb mRNA levels: effects of strength training. Med Sci Sports Exerc 39:289–297CrossRefPubMedGoogle Scholar
  23. Hulmi JJ, Ahtiainen JP, Selänne H, Volek JS, Häkkinen K, Kovanen V, Mero AA (2008a) Androgen receptors and testosterone in men—effects of protein ingestion, resistance exercise and fiber type. J Steroid Biochem Mol Biol 110:130–137CrossRefPubMedGoogle Scholar
  24. Hulmi JJ, Kovanen V, Lisko I, Selänne H, Mero AA (2008b) The effects of whey protein on myostatin and cell cycle-related gene expression responses to a single heavy resistance exercise bout in trained older men. Eur J Appl Physiol 102:205–213CrossRefPubMedGoogle Scholar
  25. Hulmi JJ, Kovanen V, Selänne H, Kraemer WJ, Häkkinen K, Mero AA (2009a) Acute and long-term effects of resistance exercise with or without protein ingestion on muscle hypertrophy and gene expression. Amino Acids 37:297–308CrossRefPubMedGoogle Scholar
  26. Hulmi JJ, Tannerstedt J, Selänne H, Kainulainen H, Kovanen V, Mero AA (2009b) Resistance exercise with whey protein ingestion affects mTOR signaling pathway and myostatin in men. J Appl Physiol 106:1720–1729CrossRefPubMedGoogle Scholar
  27. Iversen PO, Stokland A, Rolstad B, Benestad HB (1994) Adrenaline-induced leucocytosis: recruitment of blood cells from rat spleen, bone marrow and lymphatics. Eur J Appl Physiol Occup Physiol 68:219–227CrossRefPubMedGoogle Scholar
  28. Jagels MA, Hugli TE (1992) Neutrophil chemotactic factors promote leukocytosis. A common mechanism for cellular recruitment from bone marrow. J Immunol 148:1119–1128PubMedGoogle Scholar
  29. Kappel M, Hansen MB, Diamant M, Jorgensen JO, Gyhrs A, Pedersen BK (1993) Effects of an acute bolus growth hormone infusion on the human immune system. Horm Metab Res 25:579–585CrossRefPubMedGoogle Scholar
  30. Kerksick C, Harvey T, Stout J, Campbell B, Wilborn C, Kreider R, Kalman D, Ziegenfuss T, Lopez H, Landis J, Ivy JL, Antonio J (2008) International society of sports nutrition position stand: nutrient timing. J Int Soc Sports Nutr 5:17CrossRefPubMedGoogle Scholar
  31. Komi PV, Bosco C (1978) Utilization of stored elastic energy in leg extensor muscles by men and women. Med Sci Sports 10:261–265PubMedGoogle Scholar
  32. Kraemer WJ, Clemson A, Triplett NT, Bush JA, Newton RU, Lynch JM (1996) The effects of plasma cortisol elevation on total and differential leukocyte counts in response to heavy-resistance exercise. Eur J Appl Physiol Occup Physiol 73:93–97CrossRefPubMedGoogle Scholar
  33. Kraemer WJ, Adams K, Cafarelli E, Dudley GA, Dooly C, Feigenbaum MS, Fleck SJ, Franklin B, Fry AC, Hoffman JR, Newton RU, Potteiger J, Stone MH, Ratamess NA, Triplett-McBride T, American College of Sports Medicine (2002) American college of sports medicine position stand. Progression models in resistance training for healthy adults. Med Sci Sports Exerc 34:364–380CrossRefPubMedGoogle Scholar
  34. Kreider RB, Earnest CP, Lundberg J, Rasmussen C, Greenwood M, Cowan P, Almada AL (2007) Effects of ingesting protein with various forms of carbohydrate following resistance-exercise on substrate availability and markers of anabolism, catabolism, and immunity. J Int Soc Sports Nutr 4:18CrossRefPubMedGoogle Scholar
  35. Krzywkowski K, Petersen EW, Ostrowski K, Kristensen JH, Boza J, Pedersen BK (2001) Effect of glutamine supplementation on exercise-induced changes in lymphocyte function. Am J Physiol Cell Physiol 281:C1259–C1265PubMedGoogle Scholar
  36. Lagranha CJ, Levada-Pires AC, Sellitti DF, Procopio J, Curi R, Pithon-Curi TC (2008) The effect of glutamine supplementation and physical exercise on neutrophil function. Amino Acids 34:337–346CrossRefPubMedGoogle Scholar
  37. Malinowski K, Shock EJ, Rochelle P, Kearns CF, Guirnalda PD, McKeever KH (2006) Plasma beta-endorphin, cortisol and immune responses to acute exercise are altered by age and exercise training in horses. Equine Vet J Suppl (36):267–273Google Scholar
  38. Mayhew DL, Thyfault JP, Koch AJ (2005) Rest-interval length affects leukocyte levels during heavy resistance exercise. J Strength Cond Res 19:16–22CrossRefPubMedGoogle Scholar
  39. McCarthy DA, Macdonald I, Grant M, Marbut M, Watling M, Nicholson S, Deeks JJ, Wade AJ, Perry JD (1992) Studies on the immediate and delayed leucocytosis elicited by brief (30-min) strenuous exercise. Eur J Appl Physiol Occup Physiol 64:513–517CrossRefPubMedGoogle Scholar
  40. McFarlin BK, Flynn MG, Phillips MD, Stewart LK, Timmerman KL (2005) Chronic resistance exercise training improves natural killer cell activity in older women. J Gerontol A Biol Sci Med Sci 60:1315–1318PubMedGoogle Scholar
  41. Mitchell JB, Pizza FX, Paquet A, Davis BJ, Forrest MB, Braun WA (1998) Influence of carbohydrate status on immune responses before and after endurance exercise. J Appl Physiol 84:1917–1925PubMedGoogle Scholar
  42. Natale VM, Brenner IK, Moldoveanu AI, Vasiliou P, Shek P, Shephard RJ (2003) Effects of three different types of exercise on blood leukocyte count during and following exercise. Sao Paulo Med J 121:9–14CrossRefPubMedGoogle Scholar
  43. Neves Sda C Jr, Lima RM, Simoes HG, Marques MC, Reis VM, de Oliveira RJ (2009) Resistance exercise sessions do not provoke acute immunosuppression in older women. J Strength Cond Res 23:259–265PubMedGoogle Scholar
  44. Nieman DC, Henson DA, Sampson CS, Herring JL, Suttles J, Conley M, Stone MH, Butterworth DE, Davis JM (1995) The acute immune response to exhaustive resistance exercise. Int J Sports Med 16:322–328CrossRefPubMedGoogle Scholar
  45. Paulsen G, Benestad HB, Strom-Gundersen I, Morkrid L, Lappegard KT, Raastad T (2005) Delayed leukocytosis and cytokine response to high-force eccentric exercise. Med Sci Sports Exerc 37:1877–1883CrossRefPubMedGoogle Scholar
  46. Peake JM, Suzuki K, Wilson G, Hordern M, Nosaka K, Mackinnon L, Coombes JS (2005) Exercise-induced muscle damage, plasma cytokines, and markers of neutrophil activation. Med Sci Sports Exerc 37:737–745CrossRefPubMedGoogle Scholar
  47. Pedersen BK, Hoffman-Goetz L (2000) Exercise and the immune system: regulation, integration, and adaptation. Physiol Rev 80:1055–1081PubMedGoogle Scholar
  48. Pedersen BK, Ullum H (1994) NK cell response to physical activity: possible mechanisms of action. Med Sci Sports Exerc 26:140–146CrossRefPubMedGoogle Scholar
  49. Pizza FX, Davis BH, Henrickson SD, Mitchell JB, Pace JF, Bigelow N, DiLauro P, Naglieri T (1996) Adaptation to eccentric exercise: effect on CD64 and CD11b/CD18 expression. J Appl Physiol 80:47–55PubMedGoogle Scholar
  50. Raastad T, Risoy BA, Benestad HB, Fjeld JG, Hallen J (2003) Temporal relation between leukocyte accumulation in muscles and halted recovery 10–20 h after strength exercise. J Appl Physiol 95:2503–2509PubMedGoogle Scholar
  51. Rall LC, Roubenoff R, Cannon JG, Abad LW, Dinarello CA, Meydani SN (1996) Effects of progressive resistance training on immune response in aging and chronic inflammation. Med Sci Sports Exerc 28:1356–1365PubMedGoogle Scholar
  52. Ramel A, Wagner KH, Elmadfa I (2003) Acute impact of submaximal resistance exercise on immunological and hormonal parameters in young men. J Sports Sci 21:1001–1008CrossRefPubMedGoogle Scholar
  53. Risoy BA, Raastad T, Hallen J, Lappegard KT, Baeverfjord K, Kravdal A, Siebke EM, Benestad HB (2003) Delayed leukocytosis after hard strength and endurance exercise: aspects of regulatory mechanisms. BMC Physiol 3:14CrossRefPubMedGoogle Scholar
  54. Rochira V, Zirilli L, Madeo B, Maffei L, Carani C (2009) Testosterone action on erythropoiesis does not require its aromatization to estrogen: insights from the testosterone and estrogen treatment of two aromatase-deficient men. J Steroid Biochem Mol Biol 113:189–194CrossRefPubMedGoogle Scholar
  55. Saxton JM, Claxton D, Winter E, Pockley AG (2003) Peripheral blood leucocyte functional responses to acute eccentric exercise in humans are influenced by systemic stress, but not by exercise-induced muscle damage. Clin Sci (Lond) 104:69–77CrossRefGoogle Scholar
  56. Schmidt KG, Rasmussen JW (1984) Exercise-induced changes in the in vivo distribution of 111In-labelled platelets. Scand J Haematol 32:159–166PubMedGoogle Scholar
  57. Schumacher YO, Schmid A, Grathwohl D, Bultermann D, Berg A (2002) Hematological indices and iron status in athletes of various sports and performances. Med Sci Sports Exerc 34:869–875CrossRefPubMedGoogle Scholar
  58. Sedliak M, Finni T, Cheng S, Kraemer WJ, Häkkinen K (2007) Effect of time-of-day-specific strength training on serum hormone concentrations and isometric strength in men. Chronobiol Int 24:1159–1177CrossRefPubMedGoogle Scholar
  59. Segal JB, Moliterno AR (2006) Platelet counts differ by sex, ethnicity, and age in the united states. Ann Epidemiol 16:123–130CrossRefPubMedGoogle Scholar
  60. Senchina DS, Kohut ML (2007) Immunological outcomes of exercise in older adults. Clin Interv Aging 2:3–16CrossRefPubMedGoogle Scholar
  61. Shephard RJ, Verde TJ, Thomas SG, Shek P (1991) Physical activity and the immune system. Can J Sport Sci 16:169–185PubMedGoogle Scholar
  62. Simonson SR (2001) The immune response to resistance exercise. J Strength Cond Res 15:378–384CrossRefPubMedGoogle Scholar
  63. Simonson SR, Jackson CG (2004) Leukocytosis occurs in response to resistance exercise in men. J Strength Cond Res 18:266–271CrossRefPubMedGoogle Scholar
  64. Yamada M, Suzuki K, Kudo S, Totsuka M, Nakaji S, Sugawara K (2002) Raised plasma G-CSF and IL-6 after exercise may play a role in neutrophil mobilization into the circulation. J Appl Physiol 92:1789–1794PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Juha J. Hulmi
    • 1
  • T. Myllymäki
    • 1
  • M. Tenhumäki
    • 1
  • N. Mutanen
    • 1
  • R. Puurtinen
    • 1
  • G. Paulsen
    • 2
  • A. A. Mero
    • 1
  1. 1.Department of Biology of Physical Activity, Neuromuscular Research CenterUniversity of JyväskyläJyväskyläFinland
  2. 2.Norwegian School of Sport SciencesOsloNorway

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