European Journal of Applied Physiology

, Volume 109, Issue 1, pp 67–72 | Cite as

Do sessions of cryostimulation have influence on white blood cell count, level of IL6 and total oxidative and antioxidative status in healthy men?

  • Anna Lubkowska
  • Zbigniew SzygulaEmail author
  • Andrzej J. Klimek
  • Masafumi Torii
Original Article


The influence of extremely low temperatures on the human body and physiological reactions are not fully recognized. It has been postulated that cryostimulation could modify immunological reactions, leukocytes mobilization and levels of cytokines. The aim of this research was to estimate the influence of a ten sessions 3-min-long exposures to cryogenic temperature (−130°C) on the white blood cell (WBC) count, level of IL6 and the total oxidative and antioxidative status in 15 young, clinically healthy men. Blood samples were obtained in the morning before cryostimulation, again 30 min after treatment and the next day in the morning, both during the first and tenth session. The WBC count, level of IL6 and total lipid peroxides as the total oxidative status and the total antioxidative status (TAS), were measured. After completing a total of ten whole-body therapy sessions a significant increase in WBC count, especially lymphocytes and monocytes was noted. There was an increase in level of IL6 after first and the last cryostimulation the most pronounced after tenth session. On the contrary the TAS level decreased significant after the treatment. It was concluded that repeated expositions to extremely low temperatures use in cryostimulation have mobilization effect on immunological system.


Cryostimulation White blood cell count Interleukin-6 Total oxidative and antioxidative capacity 



This paper was supported by the Ministry of Science and Higher Education, Grant no N N404 027235. We are very grateful to Ms. Danuta Charland for correcting the English grammar and style of the final version of the manuscript.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Anna Lubkowska
    • 1
    • 2
  • Zbigniew Szygula
    • 3
    Email author
  • Andrzej J. Klimek
    • 4
  • Masafumi Torii
    • 5
  1. 1.Department of Physiology, Faculty of Natural SciencesSzczecin UniversitySzczecinPoland
  2. 2.Chair and Department of Biochemistry and Medical ChemistryPomeranian Medical UniversitySzczecinPoland
  3. 3.Department of Sports MedicineUniversity School of Physical EducationKrakowPoland
  4. 4.Institute of Human PhysiologyUniversity School of Physical EducationKrakowPoland
  5. 5.Division of Physiological and Biochemical Adaptation, Department of Biological Functions and Engineering, Graduate School of Life Science and Systems EngineeringKyushu Institute of TechnologyKitakyushuJapan

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