European Journal of Applied Physiology

, Volume 107, Issue 6, pp 697–706 | Cite as

Endurance training accelerates exhaustive exercise-induced mitochondrial DNA deletion and apoptosis of left ventricle myocardium in rats

  • Chi-Chang Huang
  • Tien-Jen Lin
  • Chun-Chieh Chen
  • Wan-Teng Lin
Original Article

Abstract

Even though exhaustive exercise-induced oxidative stress increases the risk of tissue damage, regular endurance training is widely assumed to improve cardiac function and protects against heart disease. We tested the hypothesis that an endurance training program prevents exhaustive exercise-induced increases in cardiac dysfunction and apoptosis in left ventricle (LV). Thirty-two male Sprague-Dawley rats were randomly divided into four groups: sedentary control (C), trained (T), exhaustively exercised (E), and trained plus exhaustively exercised (TE). Rats in T and TE groups ran on a motorized treadmill for 12 weeks. Rats in groups E and TE performed an exhaustive running test on a treadmill. The main effects of training were indicated by increased running time to exhaustion (80 ± 5 and 151 ± 13 min for groups E and TE, respectively, P = 0.0001), myocardial hypertrophy (0.38% and 0.47% for untrained and trained rats, respectively, P = 0.0002), decreased LV ejection fraction (88% and 71% for untrained and trained rats, respectively, P < 0.0001), accelerated mitochondrial DNA 4834-bp large deletion (mtDNA4834 deletion), and up-regulated protein levels of heat shock protein-70, cytochrome C, cleaved capsase-3, and cleaved PARP in LV following a bout of exhaustive exercise. Contrary to our hypothesis, these results suggest that endurance training induced significant impairment of regional systolic and diastolic LV myocardial function and ejection fraction in rats. Our findings show that endurance training accelerates exhaustive exercise-induced mtDNA4834 deletion and apoptosis in the LV.

Keywords

Endurance training Exhaustive exercise Left ventricular Mitochondrial DNA deletion Apotosis 

Notes

Acknowledgments

This study was supported by the National Science Council of Taiwan (NSC-96-2413-H-029-003 and NSC-97-2410-H029-037-MY2). We thank Drs. Ming-Dar Tsa (Department of Neurological Surgery, Shin Kong Wu Ho-Su Memorial Hospital) and Shiow-Chwen Tsai (Taipei Physical Education College) for their technical support.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Chi-Chang Huang
    • 1
  • Tien-Jen Lin
    • 2
    • 3
  • Chun-Chieh Chen
    • 1
  • Wan-Teng Lin
    • 4
  1. 1.Agricultural Biotechnology Research CenterAcademia SinicaTaipeiTaiwan, ROC
  2. 2.Division of Neurosurgery, Department of SurgeryMunicipal Wan Fang HospitalTaipeiTaiwan, ROC
  3. 3.Graduate Institute of Injury PreventionTaipei Medical UniversityTaipeiTaiwan, ROC
  4. 4.Department of Hospitality Management, College of AgricultureTunghai UniversityTaichung CityTaiwan, ROC

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