European Journal of Applied Physiology

, Volume 107, Issue 5, pp 587–595 | Cite as

Effects of tocotrienol-rich fraction on exercise endurance capacity and oxidative stress in forced swimming rats

Original Article


The present study aimed to examine the effects of tocotrienol-rich fraction (TRF) on exercise endurance and oxidative stress in forced swimming rats. Rats fed on isocaloric diet were orally given 25 (TRF-25) and 50 (TRF-50) mg/kg of TRF, or 25 mg/kg d-α-tocopherol (T-25) whilst the control group received only the vehicle for 28 days, followed by being forced to undergo swimming endurance tests, with measurements taken of various biochemical parameters, including blood glucose, lactate and urea nitrogen, glycogen, total antioxidant capacity, antioxidant enzymes, thiobarbituric acid-reactive substances (TBARS), and protein carbonyl. Results showed that the TRF-treated animals (268.0 ± 24.1 min for TRF-25 and 332.5 ± 24.3 min for TRF-50) swam significantly longer than the control (135.5 ± 32.9 min) and T-25-treated (154.1 ± 36.4 min) animals, whereas there was no difference in the performance between the T-25 and control groups. The TRF-treated rats also showed significantly higher concentrations of liver glycogen, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as of muscle glycogen and SOD than the control and the T-25-treated animals, but lower levels in blood lactate, plasma and liver TBARS, and liver and muscle protein carbonyl. Taken together, these results suggest that TRF is able to improve the physiological condition and reduce the exercise-induced oxidative stress in forced swimming rats.


Tocotrienol-rich fraction α-Tocopherol Exercise endurance Oxidative stress 



The authors would like to thank Mr. David Ho (Carotech Ltd., Malaysia) for generously providing the tocotrienol-rich fraction of palm oil for use in this study.


  1. Abe T, Takiguchi Y, Tamura M, Shimura J, Yamazaki K (1995) Effect of vespa amino acid mixture (VAAM) isolated from hornet larval saliva and modified VMM nutrients on endurance exercise in swimming mice improvement in performance and changes of blood lactate and glucose. Jpn J Physiol Fitness Sports Med 44:225–238Google Scholar
  2. Aikawa KM, Quintanilha AT, de Lumen BO, Brooks GA, Packer L (1984) Exercise endurance-training alters vitamin E tissue levels and red blood cell hemolysis in rodents. Biosci Rep 4:253–257CrossRefPubMedGoogle Scholar
  3. Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, Wiley RL (2000) Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc 32:1576–1581CrossRefPubMedGoogle Scholar
  4. Andrade FH, Reid MB, Allen DG, Westerblad H (1998) Effect of hydrogen peroxide and dithiothreitol on contractile function of single skeletal muscle fibres from the mouse. J Physiol (Lond) 509:565–575CrossRefGoogle Scholar
  5. Ashton T, Rowlands CC, Jones E, Young IS, Jackson SK, Davies B et al (1998) Electron spin resonance spectroscopic detection of oxygen centred radicals in human serum following exhaustive exercise. Eur J Appl Physiol Occup Physiol 77:498–502CrossRefPubMedGoogle Scholar
  6. Beers RF Jr, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140PubMedGoogle Scholar
  7. Bejma J, Ji LL (1999) Aging and acute exercise enhance free radical generation in rat skeletal muscle. J Appl Physiol 87:465–470PubMedGoogle Scholar
  8. Bieri JG, Anderson AA (1960) Peroxidation of lipids in tissue homogenates as related to vitamin E. Arch Biochem Biophys 90:105–110CrossRefGoogle Scholar
  9. Bonen A (2000) Lactate transporters (MCT proteins) in heart and skeletal muscles. Med Sci Sports Exerc 32:778–789CrossRefPubMedGoogle Scholar
  10. Brooks GA (2000) Intra- and extra-cellular lactate shuttles. Med Sci Sports Exec 32:790–799CrossRefGoogle Scholar
  11. Caroll NV, Longley RW, Roe JH (1956) The determination of glycogen in liver and muscle by use of anthrone reagent. J Biol Chem 220:583–593Google Scholar
  12. Chiaradia E, Avellini L, Rueca F, Spaterna A, Porciello F, Antonioni MT et al (1988) Physical exercise, oxidative stress and muscle damage in racehorses. Comp Biochem Physiol B 119:833–836CrossRefGoogle Scholar
  13. Ciocoiu M, Badescu M, Paduraru I (2007) Protecting antioxidative effects of vitamins E and C in experimental physical stress. J Physiol Biochem 63:187–194CrossRefPubMedGoogle Scholar
  14. Davies KJA, Quintanilha AT, Brooks GA, Packer L (1982) Free radicals and tissue damage produced by exercise. Biochem Biophys Res Commun 107:1198–1205CrossRefPubMedGoogle Scholar
  15. Ficicilar H, Zergeroglu AM, Tekin D, Eroz G (2003) The effects of acute exercise on plasma antioxidant status and platelet response. Thromb Res 111:267–271CrossRefPubMedGoogle Scholar
  16. Ghiselli A, Serafini M, Natella F, Scaccini C (2000) Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Rad Biol Med 29:1106–1114CrossRefPubMedGoogle Scholar
  17. Goh SH, Hew NF, Norhanom AW, Yadav M (1994) Inhibition of tumour promotion by various palm oil tocotrienols. Int J Cancer 57:529–531CrossRefPubMedGoogle Scholar
  18. Higuchi M, Cartier LJ, Chen M, Holloszy JO (1985) Superoxide dismutase and catalase in skeletal muscle: adaptive response to exercise. J Gerontol A Biol Sci Med Sci 40:281–286Google Scholar
  19. Hong H, Johnson P (1995) Antioxidant enzyme activities and lipid peroxidation levels in exercised and hypertensive rat tissues. Int J Biochem Cell Biol 27:923–931CrossRefPubMedGoogle Scholar
  20. Jenkins RR, Friedland R, Howald H (1984) The relationship of oxygen uptake to superoxide dismutase and catalase activity in human skeletal muscle. Int J Sports Med 5:11–14CrossRefPubMedGoogle Scholar
  21. Ji LL (1993) Antioxidant enzyme response to exercise and aging. Med Sci Sports Exerc 25:225–231PubMedGoogle Scholar
  22. Ji LL (1999) Antioxidants and oxidative stress in exercise. Proc Soc Exp Biol Med 222:283–292CrossRefPubMedGoogle Scholar
  23. Kamat JP, Devasagayam TPA (1995) Tocotrienols from palm oil as potent inhibitors or lipid peroxidation and protein oxidation in rat brain mitochondria. Neurosci Lett 195:179–182CrossRefPubMedGoogle Scholar
  24. Kimura YM, Kubo M, Tani T, Arichi S, Okuda H (1981) Studies on Scutellariae radix IV: effects on lipid peroxidation in rat liver. Chem Pharm Bull 29:2610–2617Google Scholar
  25. Kumar CT, Reddy VK, Prasad M, Thyagaraju K, Reddanna P (1992) Dietary supplementation of vitamin E protects heart tissue from exercise-induced oxidant stress. Mol Cell Biochem 111:109–115CrossRefPubMedGoogle Scholar
  26. Leibovitz BE, Hu ML, Tappel AL (1990) Dietary supplements of vitamin E, beta carotene, coenzyme Q10 and selenium protect tissues against lipid peroxidation in rat tissue slices. Lipids 25:125–129CrossRefPubMedGoogle Scholar
  27. Levine RL, Galand D, Oliver CN (1990) Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186:464–478CrossRefPubMedGoogle Scholar
  28. Lew H, Pyke S, Quintanilha A (1985) Changes in the glutathione status of plasma, liver and muscle following exhaustive exercise in rats. FEBS Lett 185:262–266CrossRefPubMedGoogle Scholar
  29. Maniam S, Mohamed N, Shuid AN, Soelaiman IN (2008) Palm tocotrienol exerted better antioxidant activities in bone than alpha-tocopherol. Basic Clin Pharmacol Toxicol 103:55–60CrossRefPubMedGoogle Scholar
  30. Meydani M, Evans WJ, Handelman G, Biddle L, Fielding RA, Meydani SN et al (1993) Protective effect of vitamin E on exercise-induced oxidative damage in young and older adults. Am J Physiol 264:R992–R998PubMedGoogle Scholar
  31. Minhajuddin M, Beg ZH, Iqbal J (2005) Hypolipidemic and antioxidant properties of tocotrienol rich fraction isolated from rice bran oil in experimentally induced hyperlipidemic rats. Food Chem Toxicol 43:747–753CrossRefPubMedGoogle Scholar
  32. Nesaretnam K, Devasagayam TPA, Singh BB, Basiron Y (1993) Influence of palm oil or its tocotrienol-rich fraction on the lipid peroxidation potential of rat liver mitochondria and microsomes. Biochem Mol Biol Inter 30:159–167Google Scholar
  33. Nieman DC, Henson D, McAnulty SR, McAnulty L, Swick NS, Utter AC et al (2002) Influence of vitamin C supplementation on oxidative and immune changes after an ultra-marathon. J Appl Physiol 92:1970–1977PubMedGoogle Scholar
  34. Novelli GP, Bracciotti G, Falsini S (1990) Spin-trappers and vitamin E prolong endurance to muscle fatigue in mice. Free Rad Biol Med 8:9–13CrossRefPubMedGoogle Scholar
  35. Osakada F, Hashino A, Kume T, Katsuki H, Kaneko S, Akaike A (2004) α-Tocotrienol provides the most potent neuroprotection among vitamin E analogs on cultured striatal neurons. Neuropharmacol 47:904–915CrossRefGoogle Scholar
  36. Polidori MC, Mecocci P, Cherubin A, Senin U (2000) Physical activity and oxidative stress during aging. Int J Sports Med 21:154–157CrossRefPubMedGoogle Scholar
  37. Qureshi AA, Bradlow BA, Brace L, Manganello J, Peterson DM, Pearce BC et al (1995) Response of hypercholesterolemic subjects to administration of tocotrienols. Lipids 30:1171–1177CrossRefPubMedGoogle Scholar
  38. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad Biol Med 26:1231–1237CrossRefPubMedGoogle Scholar
  39. Reznick AZ, Steinhagen-Thiessen E, Gershon D (1982) The effect of exercise on enzyme activities in cardiac muscles of mice of various ages. Biochem Med 28:347–352CrossRefPubMedGoogle Scholar
  40. Robertson JD, Maughan RJ, Duthie GG, Morrice PC (1991) Increased blood antioxidant system of runners in response to training load. Clin Sci (Colch) 80:1333–1336Google Scholar
  41. Rossi AL, Blostein-Fujii A, DiSilvesto RA (2000) Soy beverage consumption by young men: increased plasma total antioxidant status and decreased acute, exercise-induced muscle damage. J Nutr Func Med Foods 3:279–291Google Scholar
  42. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG (1973) Selenium: biochemical role as a component of glutathione peroxidase. Science 179:588–590CrossRefPubMedGoogle Scholar
  43. Salminen A, Vihko V (1983) Endurance training reduces the susceptibility of mouse skeletal muscle to lipid peroxidation in vitro. Acta Physiol Scand 177:105–110Google Scholar
  44. Salminen A, Kainulainen H, Vihko V (1984) Endurance training and antioxidants of lung. Experientia 40:822–823CrossRefPubMedGoogle Scholar
  45. Sen CK, Atalay M, Agren J, Laaksonnen DE, Hanninen O (1997) Fish oil and vitamin E supplementation in oxidative stress at rest and after physical exercise. J Appl Physiol 83:189–195PubMedGoogle Scholar
  46. Sen CK, Khanna S, Roy S, Packer L (2000) Molecular basis of vitamin E action. Tocotrienol potently inhibits glutamate-induced pp60(c-Src) kinase activation and death of HT4 neuronal cells. J Biol Chem 275:13049–13055CrossRefPubMedGoogle Scholar
  47. Serbinova B, Kagan Y, Han D, Packer L (1991) Free radical recycling and intramembrane mobility in the antioxidant properties of alpha-tocopherol and alpha-tocotrienol. Free Rad Biol Med 10:263–275CrossRefPubMedGoogle Scholar
  48. Somani SM, Ravi R, Rybak LP (1995) Effect of exercise training on antioxidant system in brain regions of rat. Pharmacol Biochem Behav 50:635–639CrossRefPubMedGoogle Scholar
  49. Sun M, Zigman S (1978) An improved spectrophotometric assay for superoxide dismutase based on epinephrine autoxidation. Anal Biochem 90:81–89CrossRefPubMedGoogle Scholar
  50. Takahashi K, Loo G (2004) Disruption of mitochondria during tocotrienol-induced apoptosis in MDA-MB-231 human breast cancer cells. Biochem Pharmacol 67:315–324CrossRefPubMedGoogle Scholar
  51. Tiidus PM, Behrens WA, Madere R, Kim JJ, Houston ME (1993) Effects of vitamin E status and exercise training on tissue lipid peroxidation based on two methods of assessment. Nutr Res 13:219–224CrossRefGoogle Scholar
  52. Wu SJ, Ng LT (2007) Antioxidant and antihepatoma activities of palm oil extract. J Food Lipids 14:122–137CrossRefGoogle Scholar
  53. Wu SJ, Liu PL, Ng LT (2008) Tocotrienol-rich fraction of palm oil exhibits anti-inflammatory property by suppressing the expression of inflammatory mediators in human monocytic cells. Mol Nutr Food Res 52:921–929CrossRefPubMedGoogle Scholar
  54. Zoppi CC, Hohl R, Silva FC, Lazarim LF, Neto JM, Stancanneli M et al (2006) Vitamin C and E supplementation effects in professional soccer players under regular training. J Int Soc Sports Nutr 3:37–44CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Ping Tin Enterprise Co., Ltd.KaohsiungTaiwan
  2. 2.Internal Medicine DepartmentKaohsiung Veteran General HospitalKaohsiungTaiwan
  3. 3.Department of Agricultural ChemistryNational Taiwan UniversityTaipei 106Taiwan

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