Successive bouts of cycling stimulates genes associated with mitochondrial biogenesis

  • Charles L. Dumke
  • J. Mark Davis
  • E. Angela Murphy
  • David C. Nieman
  • Martin D. Carmichael
  • John C. Quindry
  • N. Travis Triplett
  • Alan C. Utter
  • Sarah J. Gross Gowin
  • Dru A. Henson
  • Steven R. McAnulty
  • Lisa S. McAnulty
Original Article

DOI: 10.1007/s00421-009-1143-1

Cite this article as:
Dumke, C.L., Mark Davis, J., Angela Murphy, E. et al. Eur J Appl Physiol (2009) 107: 419. doi:10.1007/s00421-009-1143-1

Abstract

Exercise increases mRNA for genes involved in mitochondrial biogenesis and oxidative enzyme capacity. However, little is known about how these genes respond to consecutive bouts of prolonged exercise. We examined the effects of 3 h of intensive cycling performed on three consecutive days on the mRNA associated with mitochondrial biogenesis in trained human subjects. Forty trained cyclists were tested for VO2max (54.7 ± 1.1 ml kg−1 min−1). The subjects cycled at 57% wattsmax for 3 h using their own bicycles on CompuTrainer™ Pro Model trainers (RacerMate, Seattle, WA) on three consecutive days. Muscle biopsies were obtained from the vastus lateralis pre- and post-exercise on days one and three. Muscle samples were analyzed for mRNA content of peroxisome proliferator receptor gamma coactivator-1 alpha (PGC-1α), sirtuin 1 (Sirt-1), cytochrome c, and citrate synthase. Data were analyzed using a 2 (time) × 2 (day) repeated measures ANOVA. Of the mRNA analyzed, the following increased from pre to post 3 h rides: cytochrome c (P = 0.006), citrate synthase (P = 0.03), PGC-1α (P < 0.001), and Sirt-1 (P = 0.005). The following mRNA showed significant effects from days one to three: cytochrome c (P < 0.001) and citrate synthase (P = 0.01). These data show that exhaustive cycling performed on three consecutive days resulted in both acute and chronic stimuli for mRNA associated with mitochondrial biogenesis in already trained subjects. This is the first study to illustrate an increase in sirtuin-1 mRNA with acute and chronic exercise. These data contribute to the understanding of mRNA expression during both acute and successive bouts of prolonged exercise.

Keywords

Mitochondrial density Oxidative enzymes Cycling economy Endurance exercise 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Charles L. Dumke
    • 1
  • J. Mark Davis
    • 2
  • E. Angela Murphy
    • 2
  • David C. Nieman
    • 3
  • Martin D. Carmichael
    • 2
  • John C. Quindry
    • 3
  • N. Travis Triplett
    • 3
  • Alan C. Utter
    • 3
  • Sarah J. Gross Gowin
    • 3
  • Dru A. Henson
    • 3
  • Steven R. McAnulty
    • 3
  • Lisa S. McAnulty
    • 4
  1. 1.Department of Health and Human PerformanceUniversity of MontanaMissoulaUSA
  2. 2.Division of Applied Physiology, Arnold School of Public HealthUniversity of South CarolinaColumbiaUSA
  3. 3.Department of HLESAppalachian State UniversityBooneUSA
  4. 4.Department of Family and Consumer SciencesAppalachian State UniversityBooneUSA

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