European Journal of Applied Physiology

, Volume 107, Issue 2, pp 227–233

Effect of 6-day intense Kendo training on lymphocyte counts and its expression of CD95

  • Yuko Tanimura
  • Michihiro Kon
  • Kazuhiro Shimizu
  • Fuminori Kimura
  • Ichiro Kono
  • Ryuichi Ajisaka
Original Article

Abstract

This study examines the effects of 6-day intensive training on lymphocyte counts and their expression of CD95. Eight healthy Kendo athletes underwent 6-day Kendo training of about 310 min each day. Blood samples were collected at 2 weeks before (PRE), the first day (Day 1), third day (Day 3), fifth day (Day 5), and 1 week after the training period (POST) to determine lymphocyte counts and CD95 expression on CD95 lymphocytes (CD4+, CD8+) using flow cytometry. The total lymphocyte counts were significantly lower at Day 3 than at PRE. The CD8+ cell counts were significantly lower at Day 3 than at PRE. The percentage of CD95+ lymphocytes was significantly higher at Day 1 and Day 3 than at PRE. The percentage of CD8+CD95+ cells did not change significantly. The total lymphocyte counts decreased and a concomitant increase of CD95+ lymphocyte was observed, whereas the decrease in CD8+ cell counts was not associated with the increase in CD8+CD95+ cells. Therefore, short-term high-intensity exercise induced a decrease in the T lymphocyte counts without increasing in CD95+ expression.

Keywords

Intensified training Apoptosis CD95 Lymphocytopenia 

References

  1. Aizawa K, Nakahori C, Akimoto T, Kimura F, Hayashi K, Kono I, Mesaki N (2006) Changes of pituitary, adrenal and gonadal hormones during competition among female soccer players. J Sports Med Phys Fitness 46:322–327PubMedGoogle Scholar
  2. Akimoto T, Akama T, Koda Y, Waku T, Hayashi E, Tatsuno M, Sugiura K, Amano K, Kono I (1998) Effect of repetitious intense exercise training on resting salivary IgA. Jpn J Phys Fitness Sports Med 47:245–252Google Scholar
  3. Bishop NC, Walker GJ, Bowley LA, Evans KF, Molyneux K, Wallace FA, Smith AC (2005) Lymphocyte responses to influenza and tetanus toxoid in vitro following intensive exercise and carbohydrate ingestion on consecutive days. J Appl Physiol 99:1327–1335PubMedCrossRefGoogle Scholar
  4. DeRijk RH, Petrides J, Deuster P, Gold PW, Sternberg EM (1996) Changes in corticosteroid sensitivity of peripheral blood lymphocytes after strenuous exercise in humans. J Clin Endocrinol Metab 81:228–235PubMedCrossRefGoogle Scholar
  5. Dill DB, Costill DL (1974) Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol 37:247–248PubMedGoogle Scholar
  6. Fauci AS (1975) Mechanisms of corticosteroid action on lymphocyte subpopulations. I. Redistribution of circulating T and b lymphocytes to the bone marrow. Immunology 28:669–680PubMedGoogle Scholar
  7. Ferry A, Picard F, Duvallet A, Weill B, Rieu M (1990) Changes in blood leucocyte populations induced by acute maximal and chronic submaximal exercise. Eur J Appl Physiol Occup Physiol 59:435–442PubMedCrossRefGoogle Scholar
  8. Gleeson M, Bishop NC (2005) The T cell and NK cell immune response to exercise. Ann Transplant 10:43–48PubMedGoogle Scholar
  9. Green KJ (2002) Improving understanding of exercise effects on in vitro T-lymphocyte function—the role of fluorescent cell division tracking. Exerc Immunol Rev 8:101–115PubMedGoogle Scholar
  10. Green KJ, Rowbottom DG (2003) Exercise-induced changes to in vitro T-lymphocyte mitogen responses using CFSE. J Appl Physiol 95:57–63PubMedGoogle Scholar
  11. Green KJ, Croaker SJ, Rowbottom DG (2003) Carbohydrate supplementation and exercise-induced changes in T-lymphocyte function. J Appl Physiol 95:1216–1223PubMedGoogle Scholar
  12. Hayashi K, Sumi K, Horiyama K (1993) Characteristic of physical fitness in Japanese kendo champions from the view point of body composition and maximal oxygen uptake. Res J BUDO 26:25–33Google Scholar
  13. Hoffman-Goetz L, Simpson JR, Cipp N, Arumugam Y, Houston ME (1990) Lymphocyte subset responses to repeated submaximal exercise in men. J Appl Physiol 68:1069–1074PubMedGoogle Scholar
  14. Hsu TG, Hsu KM, Kong CW, Lu FJ, Cheng H, Tsai K (2002) Leukocyte mitochondria alterations after aerobic exercise in trained human subjects. Med Sci Sports Exerc 34:438–442PubMedCrossRefGoogle Scholar
  15. Ivanova SA, Semke VY, Vetlugina TP, Rakitina NM, Kudyakova TA, Simutkin GG (2007) Signs of apoptosis of immunocompetent cells in patients with depression. Neurosci Behav Physiol 37:527–530PubMedCrossRefGoogle Scholar
  16. Keen P, McCarthy DA, Passfield L, Shaker HA, Wade AJ (1995) Leucocyte and erythrocyte counts during a multi-stage cycling race (‘the Milk Race’). Br J Sports Med 29:61–65PubMedCrossRefGoogle Scholar
  17. Krzywkowski K, Petersen EW, Ostrowski K, Kristensen JH, Boza J, Pedersen BK (2001) Effect of glutamine supplementation on exercise-induced changes in lymphocyte function. Am J Physiol Cell Physiol 281:C1259–C1265PubMedGoogle Scholar
  18. Lancaster GI, Halson SL, Khan Q, Drysdale P, Wallace F, Jeukendrup AE, Drayson MT, Gleeson M (2004) Effects of acute exhaustive exercise and chronic exercise training on type 1 and type 2 T lymphocytes. Exerc Immunol Rev 10:91–106PubMedGoogle Scholar
  19. Malm C, Ekblom O, Ekblom B (2004) Immune system alteration in response to increased physical training during a five day soccer training camp. Int J Sports Med 25:471–476PubMedCrossRefGoogle Scholar
  20. Mars M, Govender S, Weston A, Naicker V, Chuturgoon A (1998) High intensity exercise: a cause of lymphocyte apoptosis? Biochem Biophys Res Commun 249:366–370PubMedCrossRefGoogle Scholar
  21. Mooren FC, Bloming D, Lechtermann A, Lerch MM, Volker K (2002) Lymphocyte apoptosis after exhaustive and moderate exercise. J Appl Physiol 93:147–153PubMedGoogle Scholar
  22. Mooren FC, Lechtermann A, Volker K (2004) Exercise-induced apoptosis of lymphocytes depends on training status. Med Sci Sports Exerc 36:1476–1483PubMedCrossRefGoogle Scholar
  23. Nieman DC (1997) Risk of upper respiratory tract infection in athletes: an epidemiologic and immunologic perspective. J Athl Train 32:344–349PubMedGoogle Scholar
  24. Nieman DC, Ahle JC, Henson DA, Warren BJ, Suttles J, Davis JM, Buckley KS, Simandle S, Butterworth DE, Fagoaga OR et al (1995) Indomethacin does not alter natural killer cell response to 2.5 h of running. J Appl Physiol 79:748–755PubMedGoogle Scholar
  25. Okutsu M, Ishii K, Niu KJ, Nagatomi R (2005) Cortisol-induced CXCR4 augmentation mobilizes T lymphocytes after acute physical stress. Am J Physiol Regul Integr Comp Physiol 288:R591–R599PubMedGoogle Scholar
  26. Pedersen BK, Ullum H (1994) NK cell response to physical activity: possible mechanisms of action. Med Sci Sports Exerc 26:140–146PubMedCrossRefGoogle Scholar
  27. Riccardi C, Zollo O, Nocentini G, Bruscoli S, Bartoli A, D’Adamio F, Cannarile L, Delfino D, Ayroldi E, Migliorati G (2000) Glucocorticoid hormones in the regulation of cell death. Therapie 55:165–169PubMedGoogle Scholar
  28. Sapin R, Schlienger JL, Gasser F, Pradignac A, Grucker D (1998) Improved specificity of a new direct assay for urinary cortisol: application in corticoid treated patients. Clin Chem Lab Med 36:855–858PubMedCrossRefGoogle Scholar
  29. Shephard RJ (2003) Adhesion molecules, catecholamines and leucocyte redistribution during and following exercise. Sports Med 33:261–284PubMedCrossRefGoogle Scholar
  30. Shinkai S, Watanabe S, Asai H, Shek PN (1996) Cortisol response to exercise and post-exercise suppression of blood lymphocyte subset counts. Int J Sports Med 17:597–603PubMedCrossRefGoogle Scholar
  31. Simpson RJ, Florida-James GD, Cosgrove C, Whyte GP, Macrae S, Pircher H, Guy K (2007a) High-intensity exercise elicits the mobilization of senescent T lymphocytes into the peripheral blood compartment in human subjects. J Appl Physiol 103:396–401PubMedCrossRefGoogle Scholar
  32. Simpson RJ, Florida-James GD, Whyte GP, Black JR, Ross JA, Guy K (2007b) Apoptosis does not contribute to the blood lymphocytopenia observed after intensive and downhill treadmill running in humans. Res Sports Med 15:157–174PubMedCrossRefGoogle Scholar
  33. Steensberg A, Morrow J, Toft AD, Bruunsgaard H, Pedersen BK (2002) Prolonged exercise, lymphocyte apoptosis and F2-isoprostanes. Eur J Appl Physiol 87:38–42PubMedCrossRefGoogle Scholar
  34. Tanimura Y, Shimizu K, Tanabe K, Otsuki T, Yamauchi R, Matsubara Y, Iemitsu M, Maeda S, Ajisaka R (2008) Exercise-induced oxidative DNA damage and lymphocytopenia in sedentary young males. Med Sci Sports Exerc 40:1455–1462PubMedCrossRefGoogle Scholar
  35. Timmons BW, Bar-Or O (2007) Lymphocyte expression of CD95 at rest and in response to acute exercise in healthy children and adolescents. Brain Behav Immun 21:442–449PubMedCrossRefGoogle Scholar
  36. Tuan TC, Hsu TG, Fong MC, Hsu CF, Tsai KK, Lee CY, Kong CW (2007) Deleterious effects of short-term high-intensity exercise on the immune function: evidence from leukocyte mitochondrial alternations and apoptosis. Br J Sports Med 42:11–15PubMedCrossRefGoogle Scholar
  37. Tvede N, Pedersen BK, Hansen FR, Bendix T, Christensen LD, Galbo H, Halkjaer-Kristensen J (1989) Effect of physical exercise on blood mononuclear cell subpopulations and in vitro proliferative responses. Scand J Immunol 29:383–389PubMedCrossRefGoogle Scholar
  38. Vider J, Lehtmaa J, Kullisaar T, Vihalemm T, Zilmer K, Kairane C, Landor A, Karu T, Zilmer M (2001) Acute immune response in respect to exercise-induced oxidative stress. Pathophysiology 7:263–270PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Yuko Tanimura
    • 1
  • Michihiro Kon
    • 1
  • Kazuhiro Shimizu
    • 1
  • Fuminori Kimura
    • 1
  • Ichiro Kono
    • 1
  • Ryuichi Ajisaka
    • 1
  1. 1.Graduate School of Comprehensive Human SciencesUniversity of TsukubaTsukubaJapan

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