Effect of intermittent hypoxic training on HIF gene expression in human skeletal muscle and leukocytes

  • Rémi Mounier
  • Vincent Pialoux
  • Belle Roels
  • Claire Thomas
  • Grégoire Millet
  • Jacques Mercier
  • Jean Coudert
  • Nicole Fellmann
  • Eric Clottes
Original Article

DOI: 10.1007/s00421-008-0928-y

Cite this article as:
Mounier, R., Pialoux, V., Roels, B. et al. Eur J Appl Physiol (2009) 105: 515. doi:10.1007/s00421-008-0928-y

Abstract

Intermittent hypoxic exposure with exercise training is based on the assumption that brief exposure to hypoxia is sufficient to induce beneficial muscular adaptations mediated via hypoxia-inducible transcription factors (HIF). We previously demonstrated (Mounier et al. Med Sci Sports Exerc 38:1410–1417, 2006) that leukocytes respond to hypoxia with a marked inter-individual variability in HIF-1α mRNA. This study compared the effects of 3 weeks of intermittent hypoxic training on hif gene expression in both skeletal muscle and leukocytes. Male endurance athletes (n = 19) were divided into an Intermittent Hypoxic Exposure group (IHE) and a Normoxic Training group (NT) with each group following a similar 3-week exercise training program. After training, the amount of HIF-1α mRNA in muscle decreased only in IHE group (−24.7%, P < 0.05) whereas it remained unchanged in leukocytes in both groups. The levels of vEGF121 and vEGF165 mRNA in skeletal muscle increased significantly after training only in the NT group (+82.5%, P < 0.05 for vEGF121; +41.2%, P < 0.05 for vEGF165). In leukocytes, only the IHE group showed a significant change in vEGF165 (−28.2%, P < 0.05). The significant decrease in HIF-1α mRNA in skeletal muscle after hypoxic training suggests that transcriptional and post-transcriptional regulations of the hif-1α gene are different in muscle and leukocytes.

Keywords

HIF-1α mRNA Natural antisense Skeletal muscle Leukocyte 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Rémi Mounier
    • 6
  • Vincent Pialoux
    • 6
  • Belle Roels
    • 1
    • 2
  • Claire Thomas
    • 3
  • Grégoire Millet
    • 1
    • 4
  • Jacques Mercier
    • 3
  • Jean Coudert
    • 6
  • Nicole Fellmann
    • 6
  • Eric Clottes
    • 5
    • 6
  1. 1.UPRES EA 3759 “Multidisciplinary Approach of Doping”MontpellierFrance
  2. 2.Centre of Sport Medicine and Human Performance, School of Sport and EducationBrunel UniversityLondonUK
  3. 3.Université Montpellier1, UFR MédecineMontpellierFrance
  4. 4.ASPIRE, Academy for Sports ExcellenceDohaQatar
  5. 5.Institut de Pharmacologie et de Biologie StructuraleToulouseFrance
  6. 6.Faculté de Médecine, Laboratoire de Physiologie-Biologie du SportClermont-FerrandFrance

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