Supplementation with an antioxidant cocktail containing coenzyme Q prevents plasma oxidative damage induced by soccer
- 332 Downloads
The aim of the study was to determine the effects of an antioxidant supplementation, which includes coenzyme Q10, on plasma and neutrophil oxidative stress and the antioxidant response after a soccer match. Nineteen voluntary male pre-professional footballers were randomly and double-blinded treated with either a multivitamin and mineral supplement (n = 8) or a placebo (n = 11). After the 3 months of supplementation, the sportsmen played a friendly soccer match of 60 min. The 3-month supplementation induced higher plasma ascorbate and coenzyme Q levels when compared to the placebo group. Antioxidant supplementation influenced plasma oxidative stress markers because they were lower in the supplemented group than in the placebo one after the match. The football match induced decreased neutrophil vitamin E levels and catalase and glutathione peroxidase activities but increased glutathione reductase activity. Antioxidant diet supplementation prevented plasma oxidative damage but did not influence the neutrophil response to a football match.
KeywordsSoccer Oxidative stress Antioxidant supplementation Coenzyme Q Plasma Neutrophils
This work has been granted by the Spanish Ministry of Science and Education (DEP2005-00238-C04-01/EOU and DEP2005-00238-C04-02/EOU) and the FEDER funding.
- Aebi HE (1984) Catalase. In: Bergmeyer HU (ed) Methods in enzymatic analysis. Verlag Chemie, Basel, pp 273–286Google Scholar
- Goldberg DM, Spooner RJ (1985) Glutathione reductase. In: Bergmeyer HU (ed) Methods in enzymatic analysis. Verlag Chemie, Basel, pp 258–265Google Scholar
- Niklowitz P, Menke T, Andler W, Okun JG (2004) Simultaneous analysis of coenzyme Q10 in plasma, erythrocytes and platelets: comparison of the antioxidant level in blood cells and their environment in healthy children and after oral supplementation in adults. Clin Chim Acta 342:219–226. doi: 10.1016/j.cccn.2003.12.020 PubMedCrossRefGoogle Scholar
- Podda M, Weber C, Traber MG, Milbradt R, Packer L (1999) Sensitive high-performance liquid chromatography techniques for simultaneous determination of tocopherols, tocotrienols, ubiquinols and ubiquinones in biological samples. In: Packer L (ed) Methods in enzymology. Oxidants and antioxidants. Academic Press, San Diego, pp 330–341CrossRefGoogle Scholar
- Radak Z, Kaneko T, Tahara S, Nakamoto H, Ohno H, Sasvari M et al (1999) The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: evidence for beneficial outcomes. Free Radic Biol Med 27:69–74. doi: 10.1016/S0891-5849(99)00038-6 PubMedCrossRefGoogle Scholar