Aerobic exercise affects C57BL/6 murine intestinal contractile function
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This study investigated the influence of a moderate exercise training program on the intestinal contractility based on the hypothesis that this organ may endure repetitive periods of ischemia-reperfusion events during moderate aerobic training (10, 25, 40, and 55 days of 60-min treadmill running at 13–21 m/min, 5 days/week). The adaptation of the animal to this program was assessed by significant increase of animal physical performance associated with a mild increase in the wet heart mass-to-body mass ratio. The endurance exercise training caused functional changes of the C57BL/6 ileum contractility, mainly causing a significant reduction of the efficacy of both the electro- (KCl) and pharmacomechanical (acetylcholine, [2-lysine]-angiotensin II, and bradykinin) couplings after 55 days of moderate treadmill running. The level of ileum lipid peroxidation, evaluated by an indirect method, significantly decreased after 10 days of moderate aerobic training, remaining at this lower level throughout the 55 days of training. Altogether, these data demonstrate that the murine ileum is an important target for aerobic moderate exercise training program by causing impairment of the contractility in response to either agonists or depolarization, and that endurance exercise exerts a remarkable protective effect against tissue oxidative stress.
KeywordsRunning training Mouse Ileum Oxidative stress Gastrointestinal smooth muscle
We are grateful to Antonio C. Souza, Mauro C. Pereira, and Nelson A. Mora for their technical assistance. This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). C.A.B. Lira was initially a fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and then from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
- Clausen JP (1977) Effect of physical training on cardiovascular adjustments to exercise in man. Physiol Rev 57:79–815Google Scholar
- Dishman RK, Berthoud HR, Booth FW, Cotman CW, Edgerton VR, Fleshner MR, Gandevia SC, Gomez-Pinilla F, Greenwood BN, Hillman CH, Kramer AF, Levin BE, Moran TH, Russo-Neustadt AA, Salamone JD, Van Hoomissen JD, Wade CE, York DA, Zigmond MJ (2006) Neurobiology of exercise. Obesity (Silver Spring) 14:345–356CrossRefGoogle Scholar
- Kemi OJ, Loennechen JP, Wisl∅ff U, Ellingsen ∅ (2002) Intensity-controlled treadmill running in mice: cardiac and skeletal muscle hypertrophy. J Appl Physiol 93:1301–1309Google Scholar
- Oltman CL, Parker JL, Adams HR, Laughlin MH (1992) Effects of exercise training on vasomotor reactivity of porcine coronary arteries. Am J Physiol 263:372–382Google Scholar
- Rembold CM (1996) Electromechanical and pharmacomechanical coupling. In: Bárány M (ed) Biochemistry of smooth muscle contraction, 1st edn. Academic Press, San Diego, pp 227–237Google Scholar
- Unsal MA, Imamoglu M, Kadioglu M, Aydin S, Ulku C, Kesim M, Alver A, Kalyoncu NI, Yaris E, Bozkaya H (2006) The acute alterations in biochemistry, morphology, and contractility of rat-isolated terminal ileum via increased intra-abdominal pressure. Pharmacol Res 53:135–141PubMedCrossRefGoogle Scholar