European Journal of Applied Physiology

, Volume 99, Issue 6, pp 585–591 | Cite as

Acute effects of hyperglycaemia with and without exercise on endothelial function in healthy young men

Original Article

Abstract

Post-prandial hyperglycaemia impairs endothelial function as evaluated by brachial artery flow-mediated dilation (FMD). Exercise is an intervention to protect against cardiovascular disease and to improve FMD. In this study, we examined whether the effect of acute hyperglycaemia on endothelial function in healthy young men is restored by aerobic exercise. Using a counterbalanced, randomized crossover design, we measured the brachial artery FMD at baseline and 1, 2, 3 and 4 h after 75 g glucose ingestion in 11 healthy young men, with and without a single bout of aerobic exercise. Brachial artery FMD declined from 11.4 ± 3.8% at baseline to 7.3 ± 3.4% 1 h after oral glucose ingestion, and returned to baseline after 4 h. When the oral glucose ingestion was followed immediately by 45 min of treadmill exercise at an intensity of 60% maximal oxygen uptake, FMD demonstrated no significant decrease (11.8 ± 2.5, 11.3 ± 2.8, 12.2 ± 2.7, 13.5 ± 3.5, and 12.6 ± 2.4% at baseline and 4 h after ingestion, respectively). The results indicate that the aerobic exercise restores the impaired FMD induced by oral glucose ingestion.

Keywords

Aerobic exercise Hyperglycaemia Endothelial dysfunction 

References

  1. Akbari CM, Saouaf R, Barnhill DF, Newman PA, LoGerfo FW, Veves A (1998) Endothelium-dependent vasodilatation is impaired in both microcirculation and macrocirculation during acute hyperglycemia. J Vasc Surg 28:687–694PubMedCrossRefGoogle Scholar
  2. Asakawa T, Matsushita S (1979) Thiobarbituric acid test for detecting lipid peroxides. Lipids 14:401–406CrossRefGoogle Scholar
  3. Ceriello A (1998) The emerging role of post-prandial hyperglycaemic spikes in the pathogenesis of diabetic complications. Diabet Med 15:188–193PubMedCrossRefGoogle Scholar
  4. Ceriello A, Bortolotti N, Crescentini A, Motz E, Lizzio S, Russo A, Ezsol Z, Tonutti L, Taboga C (1998) Antioxidant defences are reduced during the oral glucose tolerance test in normal and non-insulin-dependent diabetic subjects. Eur J Clin Invest 28:329–333PubMedCrossRefGoogle Scholar
  5. Ceriello A, Taboga C, Tonutti L, Quagliaro L, Piconi L, Bais B, Da Ros R, Motz E (2002) Evidence for an independent and cumulative effect of post-prandial hypertriglyceridemia and hyperglycemia on endothelial dysfunction and oxidative stress generation: effects of short- and long-term simvastatin treatment. Circulation 106:1211–1218PubMedCrossRefGoogle Scholar
  6. Chowienczyk PJ, Watts GF, Cockcroft JR, Ritter JM (1992) Impaired endothelium-dependent vasodilation of forearm resistance vessels in hypercholesterolaemia. Lancet 340:1430–1432PubMedCrossRefGoogle Scholar
  7. Corretti MC, Anderson TJ, Benjamin EJ, Celermajer D, Charbonneau F, Creager MA, Deanfield J, Drexler H, Gerhard-Herman M, Herrington D, Vallance P, Vita J, Vogel R (2002) Guidelines for the ultrasound assessment of endothelial-dependent flow-mediated vasodilation of the brachial artery: a report of the international brachial artery reactivity task force. J Am Coll Cardiol 39:257–265PubMedCrossRefGoogle Scholar
  8. Cosentino F, Hishikawa K, Katusic ZS, Luscher TF (1997) High glucose increases nitric oxide synthase expression and superoxide anion generation in human aortic endothelial cells. Circulation 96:25–28PubMedGoogle Scholar
  9. Dickinson S, Brand-Miller J (2005) Glycemic index, post-prandial glycemia and cardiovascular disease. Curr Opin Lipidol 16:69–75PubMedCrossRefGoogle Scholar
  10. Feener EP, King GL (1997) Vascular dysfunction in diabetes mellitus. Lancet 350:9–13CrossRefGoogle Scholar
  11. Fisslthaler B, Dimmeler S, Hermann C, Busse R, Fleming I (2000) Phosphorylation and activation of the endothelial nitric oxide synthase by fluid shear stress. Acta Physiol Scand 168:81–88PubMedCrossRefGoogle Scholar
  12. Giugliano D, Marfella R, Coppola L, Verrazzo G, Acampora R, Giunta R, Nappo F, Lucarelli C, D’Onofrio F (1997) Vascular effects of acute hyperglycemia in humans are reversed by L-arginine. Evidence for reduced availability of nitric oxide during hyperglycemia. Circulation 95:1783–1790PubMedGoogle Scholar
  13. Gori T, Harvey P, Floras JS, Parker JD (2004) Continuous therapy with nitroglycerin impairs endothelium-dependent vasodilation but does not cause tolerance in conductance arteries: a human in vivo study. J Cardiovasc Pharmacol 44:601–606PubMedCrossRefGoogle Scholar
  14. Graier WF, Simecek S, Kukovetz WR, Kostner GM (1996) High d-glucose-induced changes in endothelial Ca21/EDRF signaling are due to generation of superoxide anions. Diabetes 45:1386–1395PubMedCrossRefGoogle Scholar
  15. Gryglewski RJ, Palmer RM, Moncada S (1986) Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor. Nature 320:454–456PubMedCrossRefGoogle Scholar
  16. Hambrecht R, Adams V, Erbs S, Linke A, Krankel N, Shu Y, Baither Y, Gielen S, Thiele H, Gummert JF, Mohr FW, Schuler G (2003) Regular physical activity improves endothelial function in patients with coronary artery disease by increasing phosphorylation of endothelial nitric oxide synthase. Circulation 107:3152–3158PubMedCrossRefGoogle Scholar
  17. Hambrecht R, Wolf A, Gielen S, Linke A, Hofer J, Erbs S, Schoene N, Schuler G (2000) Effect of exercise on coronary endothelial function in patients with coronary artery disease. N Engl J Med 342:454–460PubMedCrossRefGoogle Scholar
  18. Harvey PJ, Morris BL, Kubo T, Picton PE, Su WS, Notarius CF, Floras JS (2005a) Hemodynamic after-effects of acute dynamic exercise in sedentary normotensive postmenopausal women. J Hypertens 23:285–292CrossRefGoogle Scholar
  19. Harvey PJ, Picton PE, Su WS, Morris BL, Notarius CF, Floras JS (2005b) Exercise as an alternative to oral estrogen for amelioration of endothelial dysfunction in postmenopausal women. Am Heart J 149:291–297CrossRefGoogle Scholar
  20. Hashimoto M, Akishita M, Eto M, Ishikawa M, Kozaki K, Toba K, Sagara Y, Taketani Y, Orimo H, Ouchi Y (1995) Modulation of endothelium-dependent flow-mediated dilatation of the brachial artery by sex and menstrual cycle. Circulation 92:3431–3435PubMedGoogle Scholar
  21. Heinecke JW (2003) Oxidative stress: new approaches to diagnosis and prognosis in atherosclerosis. Am J Cardiol 91:12A–16APubMedCrossRefGoogle Scholar
  22. Higashi Y, Sasaki S, Sasaki N, Nakagawa K, Ueda T, Yoshimizu A, Kurisu S, Matsuura H, Kajiyama G, Oshima T (1999) Daily aerobic exercise improves reactive hyperemia in patients with essential hypertension. Hypertension 33:591–597PubMedGoogle Scholar
  23. Ihlemann N, Rask-Madsen C, Perner A, Dominguez H, Hermann T, Kober L, Torp-Pedersen C (2003) Tetrahydrobiopterin restores endothelial dysfunction induced by an oral glucose challenge in healthy subjects. Am J Physiol Heart Circ Physiol 285: H875–H882PubMedGoogle Scholar
  24. Jain SK, Palmer M, Chen Y (1999) Effect of vitamin E and N-acetylcysteine on phosphatidylserine externalization and induction of coagulation by high-glucose-treated human erythrocytes. Metabolism 48:957–959PubMedCrossRefGoogle Scholar
  25. Joannides R, Haefeli WE, Linder L, Richard V, Bakkali EH, Thuillez C, Luscher TF (1995) Nitric oxide is responsible for flow-dependent dilatation of human peripheral conduit arteries in vivo. Circulation 91:1314–1319PubMedGoogle Scholar
  26. Jungersten L, Ambring A, Wall B, Wennmalm A (1997) Both physical fitness and acute exercise regulate nitric oxide formation in healthy humans. J Appl Physiol 82:760–764PubMedCrossRefGoogle Scholar
  27. Kawano H, Motoyama T, Hirashima O, Hirai N, Miyao Y, Sakamoto T, Kugiyama K, Ogawa H, Yasue H (1999) Hyperglycemia rapidly suppresses flow-mediated endothelium-dependent vasodilation of brachial artery. J Am Coll Cardiol 34:146–154PubMedCrossRefGoogle Scholar
  28. Kingwell BA, Sherrard B, Jennings GL, Dart AM (1997) Four weeks of cycle training increases basal production of nitric oxide from the forearm. Am J Physiol 272: H1070–H1077PubMedGoogle Scholar
  29. Konukoglu D, Hatemi H, Ozer E, Gonen S, Akcay T (1997) The erythrocyte glutathione levels during oral glucose tolerance test. J Endocrinol Invest 20:471–475PubMedGoogle Scholar
  30. Lefebvre PJ, Scheen AJ (1998) The postprandial state and risk of cardiovascular disease. Diabet Med 15: S63–S68PubMedCrossRefGoogle Scholar
  31. Ma SW, Tomlinson B, Benzie IF (2005) A study of the effect of oral glucose loading on plasma oxidant:antioxidant balance in normal subjects. Eur J Nutr 44:250–254PubMedCrossRefGoogle Scholar
  32. Maiorana A, O’Driscoll G, Cheetham C, Dembo L, Stanton K, Goodman C, Taylor R, Green D (2001) The effect of combined aerobic and resistance exercise training on vascular function in type 2 diabetes. J Am Coll Cardiol 38:860–866PubMedCrossRefGoogle Scholar
  33. Marsh SA, Coombes JS (2005) Exercise and the endothelial cell. Int J Cardiol 99:165–169PubMedCrossRefGoogle Scholar
  34. Node K, Kitakaze M, Sato H, Koretsune Y, Katsube Y, Karita M, Kosaka H, Hori M (1997) Effect of acute dynamic exercise on circulating plasma nitric oxide level and correlation to norepinephrine release in normal subjects. Am J Cardiol 79:526–528PubMedCrossRefGoogle Scholar
  35. Padilla J, Harris RA, Fly AD, Rink LD, Wallace JP (2006) The effect of acute exercise on endothelial function following a high-fat meal. Eur J Appl Physiol 98:256–262PubMedCrossRefGoogle Scholar
  36. Ross R (1986) The pathogenesis of atherosclerosis-an update. N Engl J Med 314:488–500PubMedCrossRefGoogle Scholar
  37. Rubanyi GM, Vanhoutte PM (1986) Oxygen-derived free radicals, endothelium, and responsiveness of vascular smooth muscle. Am J physiol 250: H815–H821PubMedGoogle Scholar
  38. Siafarikas A, Watts K, Beye P, Jones TW, Davis EA, Green DJ (2004) Lack of effect of oral glucose loading on conduit vessel endothelial function in healthy subjects. Clin Sci (Lond) 107:191–196CrossRefGoogle Scholar
  39. Tanriverdi H, Evrengul H, Tanriverdi S, Turgut S, Akdag B, Kaftan HA, Semiz E (2005) Improved endothelium dependent vasodilation in endurance athletes and its relation with ACE I/D polymorphism. Circ J 69:1105–1110PubMedCrossRefGoogle Scholar
  40. Tesfamariam B, Cohen RA (1992) Free radicals mediate endothelial cell dysfunction caused by elevated glucose. Am J Physiol 263:H321–H326PubMedGoogle Scholar
  41. Title LM, Cummings PM, Giddens K, Nassar BA (2000) Oral glucose loading acutely attenuates endothelium-dependent vasodilation in healthy adults without diabetes: an effect prevented by vitamins C and E. J Am Coll Cardiol 36:2185–2191PubMedCrossRefGoogle Scholar
  42. Treasure CB, Klein JL, Vita JA, Manoukian SV, Renwick GH, Selwyn AP, Ganz P, Alexander RW (1993) Hypertension and left ventricular hypertrophy are associated with impaired endothelium-mediated relaxation in human coronary resistance vessels. Circulation 87:86–93PubMedGoogle Scholar
  43. Walsh JH, Bilsborough W, Maiorana A, Best M, O’Driscoll GJ, Taylor RR, Green DJ (2003) Exercise training improves conduit vessel function in patients with coronary artery disease. J Appl Physiol 95:20–25PubMedGoogle Scholar
  44. Walther C, Gielen S, Hambrecht R (2004) The effect of exercise training on endothelial function in cardiovascular disease in humans. Exerc Sport Sci Rev 32:129–134PubMedCrossRefGoogle Scholar
  45. Williams SB, Goldfine AB, Timimi FK, Ting HH, Roddy MA, Simonson DC, Creager MA (1998) Acute hyperglycemia attenuates endothelium-dependent vasodilation in humans in vivo. Circulation 97:1695–1701PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Weili Zhu
    • 1
    • 2
  • Chongfa Zhong
    • 3
  • Yingjie Yu
    • 1
  • Keji Li
    • 1
  1. 1.Department of Nutrition and Food Hygiene, School of Public HealthPeking University Health Science CenterBeijingChina
  2. 2.Department of AnatomyCapital Institute of Physical EducationBeijingChina
  3. 3.Department of 11Institute of Space Medical EngineeringBeijingChina

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