European Journal of Applied Physiology

, Volume 99, Issue 6, pp 695–699 | Cite as

Effects of magnesium sulfate on dynamic changes of brain glucose and its metabolites during a short-term forced swimming in gerbils

  • Shiu-Min Cheng
  • Dar-Yu Yang
  • Chien-Pin Lee
  • Hung-Chuan Pan
  • Mao-Tsun Lin
  • Ssu-Hua Chen
  • Fu-Chou Cheng
Original Article

Abstract

This investigation examined the acute effects of magnesium on the dynamic changes of brain glucose, lactate, pyruvate and magnesium levels in conscious gerbils during forced swimming. Gerbils were pretreated with saline (control group) and magnesium sulfate (90 mg kg−1, intraperitoneal injection) before a 15 min forced swimming period. The basal levels of glucose, pyruvate, lactate, and magnesium in brain dialysates were 338 ± 18, 21 ± 2, 450 ± 39, and 2.1 ± 0.1 μM, respectively, with no significant difference between groups. Magnesium levels were found slightly higher (but not significant) in the magnesium-treated group. However, brain glucose and pyruvate levels in the control group decreased to about 50 and 60% of the basal level (P = 0.01) after swimming, respectively. Pretreatment with magnesium sulfate immediately increased glucose levels to about 140% of the basal level, and increased pyruvate levels to about 150% of the basal level during forced swimming (P = 0.01). Both glucose and pyruvate levels returned to the basal level after 30 min of the recovery. The lactate levels of the control group increased to about 160% of the basal level (P = 0.01) during swimming, whereas pretreatment with magnesium sulfate attenuated lactate levels to 130% of the basal level (P = 0.01). Magnesium supplementation may be beneficial because it provides an additional glucose source and may also promote the recovery of energy substrates in the brain during and after forced exercise. In order to achieve optimal physical performance, further investigation as to dosage of magnesium supplementation is needed.

Keywords

Lactate Pyruvate Microdialysis Swimming 

Notes

Acknowledgments

This study was supported by grants from Taichung Veterans General Hospital (TCVGH-957316C), and the National Science Council (NSC-94-2113-M-075A-001), Taiwan.

References

  1. Altura BM (1991) Basic biochemistry and physiology of magnesium: a brief review. Magnes Trace Elem 10:167–171PubMedGoogle Scholar
  2. Arai I, Tsuyuki Y, Shiomoto H, Satoh M, Otomo S (2000) Decreased body temperature-dependent appearance of behavioral despair in the forced swimming test in mice. Pharmacol Res 42:171–176PubMedCrossRefGoogle Scholar
  3. Auer RN (1986) Progress review: hypoglycemic brain damage. Stroke 17:699–708PubMedGoogle Scholar
  4. Bohl CH, Volpe SL (2002) Magnesium and exercise. Crit Rev Food Sci Nutr 42:533–563PubMedCrossRefGoogle Scholar
  5. Brilla LR, Gunter KB (1995) Effect of magnesium supplementation on exercise time to exhaustion. Med Exerc Nutr Health 4:230–233Google Scholar
  6. Clarkson PM (1991) Minerals: exercise performance and supplementation in athletes. J Sports Sci 9:91–116PubMedGoogle Scholar
  7. Coggan AR (1991) Plasma glucose metabolism during exercise in humans. Sports Med 11:102–124PubMedGoogle Scholar
  8. Donovan CM, Sumida KD (1997) Training enhanced hepatic gluconeogenesis: the importance for glucose homeostasis during exercise. Med Sci Sports Exerc 29:628–634PubMedGoogle Scholar
  9. Ebel H, Gunther T (1980) Magnesium metabolism: a review. J Clin Chem Clin Biochem 18:257–270PubMedGoogle Scholar
  10. Felig P, Cherif A, Minagawa A, Wahren J (1982) Hypoglycemia during prolonged exercise in normal men. N Engl J Med 306:895–900PubMedCrossRefGoogle Scholar
  11. Gotoh M, Tajima T, Suzuki Y, Ikari H, Iguchi A, Kakumu S, Hirooka Y (1998) Swimming stress that causes hyperglycemia increases in vivo release of noradrenaline, but not acetylcholine, from the hypothalamus of conscious rats. Brain Res 780:74–79PubMedCrossRefGoogle Scholar
  12. Horn TF, Engelmann M (2001) In vivo microdialysis for nonapeptides in rat brain-a practical guide. Methods 23:41–53PubMedCrossRefGoogle Scholar
  13. Ide K, Schmalbruch IK, Quistorff B, Horn A, Secher NH (2000) Lactate, glucose and O2 uptake in human brain during recovery from maximal exercise. J Physiol 522:159–164PubMedCrossRefGoogle Scholar
  14. Kemppainen J, Aalto S, Fujimoto T, Kalliokoski KK, Langsjo J, Oikonen V, Rinne J, Nuutila P, Knuuti J (2005) High intensity exercise decreases global brain glucose uptake in humans. J Physiol 568:323–332PubMedCrossRefGoogle Scholar
  15. Lin JY, Chung SY, Lin MC, Cheng FC (2002) Effects of magnesium sulfate on energy metabolites and glutamate in the cortex during focal cerebral ischemia and reperfusion in the gerbil monitored by a dual-probe microdialysis technique. Life Sci 71:803–811PubMedCrossRefGoogle Scholar
  16. Lin MC, Huang YL, Liu HW, Yang DY, Lee JB, Cheng FC (2004) Microdialysis analyzer and flame atomic absorption spectrometry in the determination of blood glucose, lactate and magnesium in gerbils subjected to cerebral ischemia/reperfusion. J Am Coll Nutr 23:556S–560SPubMedGoogle Scholar
  17. Lin YC (1988) Applied physiology of diving. Sports Med 5:41–56PubMedGoogle Scholar
  18. Lukaski HC (2000) Magnesium, zinc, and chromium nutriture and physical activity. Am J Clin Nutr 72:585S–593SPubMedGoogle Scholar
  19. Meeusen R, Piacentini MF, De Meirleir K (2001) Brain microdialysis in exercise research. Sports Med 31:965–983PubMedCrossRefGoogle Scholar
  20. Mooren FC, Golf SW, Lechtermann A, Volker K (2005) Alterations of ionized Mg2 + in human blood after exercise. Life Sci 77:1211–1225PubMedCrossRefGoogle Scholar
  21. Nybo L, Moller K, Pedersen BK, Nielsen B, Secher NH (2003) Association between fatigue and failure to preserve cerebral energy turnover during prolonged exercise. Acta Physiol Scand 179:67–74PubMedCrossRefGoogle Scholar
  22. Pisani A, Bonsi P, Picconi B, Tolu M, Giacomini P, Scarnati E (2001) Role of tonically-active neurons in the control of striatal function: cellular mechanisms and behavioral correlates. Prog Neuropsychopharmacol Biol Psychiatry 25:211–230PubMedCrossRefGoogle Scholar
  23. Poleszak E, Wlaz P, Szewczyk B, Kedzierska E, Wyska E, Librowski T, Szymura-Oleksiak J, Fidecka S, Pilc A, Nowak G (2005) Enhancement of antidepressant-like activity by joint administration of imipramine and magnesium in the forced swim test: behavioral and pharmacokinetic studies in mice. Pharmacol Biochem Behav 81:524–529PubMedCrossRefGoogle Scholar
  24. Rayssiguier Y, Guezennec CY, Durlach J (1990) New experimental and clinical data on the relationship between magnesium and sport. Magnes Res 3:93–102PubMedGoogle Scholar
  25. Saris NE, Mervaala E, Karppanen H, Khawaja JA, Lewenstam A (2000) Magnesium. An update on physiological, clinical and analytical aspects. Clin Chim Acta 294:1–26PubMedCrossRefGoogle Scholar
  26. Stendig-Lindberg G, Shapiro Y, Epstein Y, Galun E, Schonberger E, Graff E, Wacker WE (1987) Changes in serum magnesium concentration after strenuous exercise. J Am Coll Nutr 6:35–40PubMedGoogle Scholar
  27. Szabo MD, Crosby G (1988) Effect of profound hypermagnesemia on spinal cord glucose utilization in rats. Stroke 19:747–749PubMedGoogle Scholar
  28. Wacker WE, Parisi AF (1968) Magnesium metabolism. N Engl J Med 278:772–776 conclGoogle Scholar
  29. Wang L, Dong Y, Yu X, Shangguan DH, Zhao R, Han HW, Liu ZQ (2002) Analysis of glucose and lactate in dialysate from hypothalamus of rats after exhausting swimming using microdialysis. Biomed Chromatogr 16:427–431PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Shiu-Min Cheng
    • 1
  • Dar-Yu Yang
    • 2
  • Chien-Pin Lee
    • 3
  • Hung-Chuan Pan
    • 4
  • Mao-Tsun Lin
    • 1
    • 5
  • Ssu-Hua Chen
    • 3
  • Fu-Chou Cheng
    • 3
  1. 1.Institute of PhysiologyNational Yang-Ming UniversityTaipeiTaiwan
  2. 2.Department of NeurosurgeryChang Bing Show Chwan Memorial HospitalChanghuaTaiwan
  3. 3.Stem Cell Center, Department of Medical ResearchTaichung Veterans General HospitalTaichungTaiwan
  4. 4.Department of NeurosurgeryTaichung Veterans General HospitalTaichungTaiwan
  5. 5.Department of CardiologyChi-Mei Medical CenterTainanTaiwan

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