Response of antioxidant defences to oxidative stress induced by prolonged exercise: antioxidant enzyme gene expression in lymphocytes
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The response of lymphocyte and plasma antioxidant defences to a prolonged exercise as a cycling stage in a professional race was analysed. Antioxidant enzyme activities and gene expression, carbonyl derivative and MDA levels were determined in lymphocytes. Plasma levels of vitamin E, carotenes, protein carbonyl derivatives and the test d-Roms were measured. Significant increases in plasmatic carbonyls and in the test d-Roms were observed after the cycling stage. No significant differences were found in the lymphocyte MDA and carbonyl derivative levels. A significant increase was found in plasma vitamin E concentration after the cycling stage; however, the lymphocyte vitamin E concentration did not change. Significant increases were observed in lymphocyte total superoxide dismutase (SOD) activity and in the levels of CuZn-SOD and Mn-SOD isoenzymes. The moderate levels of oxidative stress in the lymphocyte induced a cellular adaptation to exercise enough to counteract the negative effects of oxidative stress.
KeywordsCycling Oxidative stress Lymphocytes Antioxidants
This work has been granted aid by the Spanish Ministry of Health (Programme of Promotion of Biomedical Research and Health Sciences, Project PI021593), the Spanish Ministry of Science and Education (DEP2005-00238-C04-02/EOU) and the FEDER funding.
- Aebi HE (1984) Catalase. In: Bergmeyer HU (ed) Methods in enzymatic analysis. Verlag Chemie, Basel, pp 273–286Google Scholar
- Burtis CA, Ashwood E (1984) Tietz textbook of clinical chemistry. WB Saunders, PhiladelphiaGoogle Scholar
- Gleeson M, Nieman DC, Pedersen BK (2004) Exercise, nutrition and immune function. In: Maughan RJ, Burke LM, Coyle EF (eds) Food, nutrition and sports performance II. Rouledge, London, pp 186–203Google Scholar
- Goldberg DM, Spooner RJ (1985) Glutathione Reductase. In: Bergmeyer HU (eds) Methods in enzymatic analysis. Verlag Chemie, Basel, pp 258–265Google Scholar
- Tauler P, Sureda A, Cases N, Aguilo A, Rodríguez-Marroyo JA, Villa G, Tur JA, Pons A (2005) Increased lymphocyte antioxidant defences in response to exhaustive exercise do not prevent oxidative damage. J Nutr Biochem (In press)Google Scholar
- Tietz N (1999) Clinical guide to laboratory tests. WB Saunders, PhiladelphiaGoogle Scholar
- Wang JS, Huang YH (2005) Effects of exercise intensity on lymphocyte apoptosis induced by oxidative stress in men. Eur J Appl Physiol 12:1–8Google Scholar
- Weeks I, Woodhead JS (1984) Chemiluminiscence assays. J Clin Immunoassay 7:82–89Google Scholar