European Journal of Applied Physiology

, Volume 98, Issue 3, pp 234–241

Creatine supplementation does not enhance submaximal aerobic training adaptations in healthy young men and women

  • T. F. Reardon
  • P. A. Ruell
  • M. A. Fiatarone Singh
  • C. H. Thompson
  • K. B. Rooney
Original Article


The benefits of dietary creatine supplementation on muscle performance are generally related to an increase in muscle phosphocreatine content. However, creatine supplementation may benefit endurance sports through increased glycogen re-synthesis following exercise. This study investigated the effect of creatine supplementation on muscle glycogen content, submaximal exercise fuel utilisation and endurance performance following 4 weeks of endurance training. Thirteen healthy, physically active, non-vegetarian subjects volunteered to take part and completed the study. Subjects were supplemented with either creatine monohydrate (CREAT, n = 7) or placebo-maltodextrin (CON, n = 6). Submaximal fuel utilisation and endurance performance were assessed before and after a 4 week endurance training program. Muscle biopsies were also collected before and following training for assessment of muscle creatine and glycogen content. Training increased quadriceps glycogen content to the same degree (∼20%) in both groups (P = 0.04). There was a significant training effect on submaximal fuel utilisation and improved endurance performance. However, there was no significant treatment effect of creatine supplementation. Creatine supplementation does not effect metabolic adaptations to endurance training.


Carbohydrate Endurance Glycogen Performance 


  1. Balsom PD, Harridge SD, Soderlund K, Sjodin B, Ekblom B (1993) Creatine supplementation per se does not enhance endurance exercise performance. Acta Physiol Scand 149:521–523PubMedGoogle Scholar
  2. Bergstrom J, Hermansen L, Hultman E, Saltin B (1967) Diet, muscle glycogen and physical performance. Acta Physiol Scand 71:140–150PubMedCrossRefGoogle Scholar
  3. Booth JM, McKenna MJ, Ruell P, Gwinn T, Davis GM, Thompson MW, Harmer AR, Hunter SK, Sutton JR (1997) Impaired calcium pump function doers not slow relaxation in human skeletal muscle after prolonged exercise. J Appl Physiol 83:511–521PubMedGoogle Scholar
  4. Conlee RK (1987) Muscle glycogen and exercise endurance: a twenty-year perspective. Exerc Sports Sci Rev 15:1–28CrossRefGoogle Scholar
  5. Derave W, Eijnde BO, Verbessem P, Ramaekers M, Van Leemputte M, Richter EA, Hespel P (2003) Combined creatine and protein supplementation in conjunction with resistance training promotes muscle GLUT-4 content and glucose tolerance in humans. J App Physiol 94:1910–1916Google Scholar
  6. Engelhardt M, Neumann G, Berbalk A, Reuter I (1998) Creatine supplementation in endurance sports. Med Sci Sports Exerc 30:1123–1129PubMedCrossRefGoogle Scholar
  7. Evans WJ, Phinney SD, Young VR (1982) Suction applied to a muscle biopsy maximizes sample size. Med Sci Sports Exerc 14:101–102PubMedGoogle Scholar
  8. Greenhaff PL, Casey A, Short AH, Harris R, Soderlund K, Hultman E (1993) Influence of oral creatine supplementation of muscle torque during repeated bouts of maximal voluntary exercise in man. Clin Sci 84:565–571PubMedGoogle Scholar
  9. Harris RC, Soderlund K, Hultman E (1992) Elevation of creatine in resting and exercised muscle of normal subjects by creatine supplementation. Clin Sci 83:367–374PubMedGoogle Scholar
  10. Hawley JA, Schabort EJ, Noakes TD, Dennis SC (1997) Carbohydrate-loading and exercise performance. An update Sports Med 24:73–81Google Scholar
  11. Holloszy JO, Booth FW (1976) Biochemical adaptations to endurance exercise in muscle. Ann Rev Physiol 38:273–291CrossRefGoogle Scholar
  12. Izquierdo M, Ibanez J, Gonzalez-Badillo JJ, Gorostiaga EM (2002) Effects of creatine supplementation on muscle power, endurance, and sprint performance. Med Sci Sports Exerc 34:332–343PubMedCrossRefGoogle Scholar
  13. Lukasi HC (1987) Methods for the assessment of human body composition: traditional and new. J Clin Nutr 46:537–556Google Scholar
  14. Nelson AG, Arnall DA, Kokkonen J, Day R, Evans J (2001) Muscle glycogen supercompensation is enhanced by prior creatine supplementation. Med Sci Sports Exerc 33:1096–1100PubMedGoogle Scholar
  15. Newman JE, Hargreaves M, Garnham A, Snow RJ (2003) Effect of creatine ingestion on glucose tolerance and insulin sensitivity in men. Med Sci Sports Exerc 35:69–74PubMedCrossRefGoogle Scholar
  16. Op ‘t Eijnde B, Richter EA, Henquin JC, Kiens B, Hespel P (2001a) Effect of creatine supplementation on creatine and glycogen content in rat skeletal muscle. Acta Physiol Scand 171:169–176CrossRefGoogle Scholar
  17. Op ‘t Eijnde B, Urso B, Richter EA, Greenhaff PL, Hespel P (2001b) Effect of oral creatine supplementation on human muscle glut4 protein content after immobilization. Diabetes 50:18–23CrossRefGoogle Scholar
  18. Peronnet F, Massicotte D (1991) Table of nonprotein respiratory quotient: an update. Can J Sport Sci 16:271–279Google Scholar
  19. Powers ME, Arnold BL, Weltman AL, Perrin DH, Mistry D, Kahler DM, Kraemer W, Volek J (2003) Creatine supplementation increases total body water without altering fluid distribution. J Athl Train 38:44–50PubMedGoogle Scholar
  20. Robinson TM, Sewell DA, Hultman E, Greenhaff PL (1999) Role of submaximal exercise in promoting creatine and glycogen accumulation in human skeletal muscle. J Appl Physiol 87:598–604PubMedGoogle Scholar
  21. Terjung RL, Clarkson P, Eichner ER, Greenhaff PL, Hespel PJ, Israel RG, Kraemer WJ, Meyer RA, Spriet LL, Tarnopolsky MA, Wagenmakers AJ, Williams MH (2000) American college of sports medicine roundtable. The physiological and health effects of oral creatine supplementation. Med Sci Sports Exerc 32:706–717PubMedCrossRefGoogle Scholar
  22. Van Loon LJ, Oosterlaar AM, Hartgens F, Hesselink MKC, Snow RJ, Wagenmakers AJM (2003) Effects of creatine loading and prolonged creatine supplementation on body composition, fuel selection, sprint and endurance performance in humans. Clin Sci 104:153–162PubMedCrossRefGoogle Scholar
  23. Van Loon LJ, Murphy R, Oosterlaar AM, Cameron-Smith D, Hargreaves M, Wagenmakers AJM, Snow R (2004) Creatine supplementation increases glycogen storage but not GLUT-4 expression in human skeletal muscle. Clin Sci 106:99–106PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • T. F. Reardon
    • 1
  • P. A. Ruell
    • 1
  • M. A. Fiatarone Singh
    • 1
    • 2
    • 3
  • C. H. Thompson
    • 4
  • K. B. Rooney
    • 1
  1. 1.School of Exercise and Sport Science, Faculty of Health SciencesUniversity of SydneyLidcombeAustralia
  2. 2.Faculty of MedicineUniversity of SydneySydneyAustralia
  3. 3.Hebrew SeniorLife and John Mayer USDA Human Nutrition Center on Aging at Tufts UniversityBostonUSA
  4. 4.Department of General Medicine, Flinders Medical CentreFlinders University of South AustraliaAdelaideAustralia

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