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European Journal of Applied Physiology

, Volume 97, Issue 5, pp 548–556 | Cite as

Protein supplementation before and after resistance training in older men

  • Darren G. Candow
  • Philip D. Chilibeck
  • Marina Facci
  • Saman Abeysekara
  • Gordon A. Zello
Original Article

Abstract

We determined the effects of protein supplementation immediately before (PRO-B) and after (PRO-A) resistance training (RT; 12 weeks) in older men (59–76 years), and whether this reduces deficits in muscle mass and strength compared to younger men (18–40 years). Older men were randomized to PRO-B (0.3 g/kg protein before RT + placebo after RT, n=9), PRO-A (placebo before + protein after RT, n=10), or PLA (placebo before and after RT, n=10). Lean tissue mass, muscle thickness of the elbow, knee, and ankle flexors and extensors, and leg and bench press strength were measured before and after RT and compared to databases of younger subjects (n=22–60). Myofibrillar protein degradation (3-methylhistidine) and bone resorption (cross-linked N-telopeptides) were also measured before and after RT. Lean tissue mass, muscle thickness (except ankle dorsi flexors), and strength increased with training (P<0.05), with little difference between groups. There were no changes in 3-methylhistidine or cross-linked N-telopeptides. Before RT, all measures were lower in the older compared to younger groups (P<0.05), except for elbow extensor muscle thickness. Following training, muscle thickness of the elbow flexors and ankle dorsi flexors and leg press strength were no longer different than the young, and elbow extensor muscle thickness was greater in the old men (P<0.05). Supplementation with protein before or after training has no effect on muscle mass and strength in older men. RT was sufficient to overcome deficits in muscle size of the elbow flexors and ankle dorsi flexors and leg press strength in older compared to younger men.

Keywords

Age Muscle Strength Catabolism Bone 

Notes

Acknowledgments

This study was supported through a research grant from Experimental and Applied Sciences, Golden, CO, USA, and by the Natural Sciences and Engineering Research Council of Canada. Funding for the weight training equipment was provided by the Saskatchewan Health Research Foundation. The authors would like to thank Dr Bernhard Juurlink, Department of Anatomy and Cell Biology at the University of Saskatchewan for the use of his lab and materials.

References

  1. Andersen LL, Tufekovic G, Zebis MK, Crameri RM, Verlaan G, Kjaer M, Suetta C, Magnusson P, Aagaard P (2005) The effect of resistance training combined with timed ingestion of protein on muscle fiber size and muscle strength. Metabolism 54:151–156PubMedCrossRefGoogle Scholar
  2. Barzel US (1995) The skeleton as an ion exchange system: implications for the role of acid–base imbalance in the genesis of osteoporosis. J Bone Miner Res 10:1431–1436PubMedCrossRefGoogle Scholar
  3. Beaumont D, Lehmann AB, James OFW (1989) Protein turnover in malnourished elderly subjects: the effects of refeeding. Age Ageing 18:235–240PubMedCrossRefGoogle Scholar
  4. Biolo G, Tipton KD, Klein S, Wolfe RR (1997) An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein. Am J Physiol Endocrinol Metab 273:E122–E129Google Scholar
  5. Bos C, Benamouzig R, Bruhat A, Roux C, Mahe S, Valensi P, Gaudichon C, Ferriere F, Rautureau J, Tome D (2000) Short-term protein and energy supplementation activates nitrogen kinetics and accretion in poorly nourished elderly subjects. Am J Clin Nutr 71:1129–1137PubMedGoogle Scholar
  6. Burke DG, Chilibeck PD, Davison KS, Candow DG, Farthing J, Palmer TS (2001) The effect of whey protein supplementation with and without creatine monohydrate combined with resistance training on lean tissue and muscle strength. Int J Sport Nutr Exerc Metab 11:349–364PubMedGoogle Scholar
  7. Burke DG, Chilibeck PD, Parise G, Candow DG, Mahoney D, Tarnopolsky MA (2003) Effect of creatine and weight training on muscle creatine performance in vegetarians. Med Sci Sports Exerc 35:1946–1955PubMedCrossRefGoogle Scholar
  8. Bylund-Fellenius AC, Ojamaa KM, Flaim KE, Li JB, Wassner SJ, Jefferson LS (1984) Protein synthesis versus energy state in contracting muscle of perfused rat hindlimb. Am J Physiol 259:E297–E305Google Scholar
  9. Campbell WW, Crim MC, Young VR, Joseph LJ, Evans WJ (1995) Effects of resistance training and dietary protein intake on protein metabolism in older adults. Am J Physiol Endocrinol Metab 31:E1143–E1153Google Scholar
  10. Candow DG, Chilibeck PD (2005) Differences in size, strength, and power of upper and lower body muscle groups in young and older men. J Gerontol Biol Sci 60:148–156Google Scholar
  11. Candow DG, Chilibeck PD, Burke DG, Davison KS, Palmer TS (2001) Effect of glutamine supplementation combined with resistance training in young men. Eur J Appl Physiol 86:142–149PubMedCrossRefGoogle Scholar
  12. Carraro F, Stuart CA, Hartl WH, Rosenblatt J, Wolfe RR (1990) Effect of exercise and recovery on muscle protein synthesis in human subjects. Am J Physiol 259:E470–E476PubMedGoogle Scholar
  13. Chilibeck PD, Calder AW, Sale DG, Webber CE (1998) A comparison of strength and muscle mass increases during resistance training in young women. Eur J Appl Physiol Occup Physiol 77:170–175PubMedCrossRefGoogle Scholar
  14. Chrusch MJ, Chilibeck PD, Chad KE, Davison KS, Burke DG (2001) Creatine supplementation combined with resistance training in older men. Med Sci Sports Exerc 33:2111–2117PubMedCrossRefGoogle Scholar
  15. Dawson-Hughes B, Harris SS, Rasmussen H, Song L, Dallal GE (2004) Effect of dietary protein supplements on calcium excretion in healthy older men and women. J Clin Endocrinol Metab 89:1169–1173PubMedCrossRefGoogle Scholar
  16. Esmarck B, Andersen JL, Olsen S, Richter EA, Mizuno M, Kjaer M (2001) Timing of postexercise protein intake is important for muscle hypertrophy with resistance training in elderly humans. J Physiol 535:301–311PubMedCrossRefGoogle Scholar
  17. Godard MP, Williamson DL, Trappe SW (2002) Oral amino-acid provision does not affect muscle strength or size in older men. Med Sci Sports Exerc 34:1126–1131PubMedCrossRefGoogle Scholar
  18. Janssen I, Heymsfield SB, Wang A, Ross R (2000) Skeletal muscle mass and distribution in 468 men and women aged 18–88 yr. J Appl Physiol 89:81–88PubMedGoogle Scholar
  19. Kerstetter JE, Mitnick ME, Gundberg CM, Caseria DM, Ellison AF, Carpenter TO, Insogna KL (1999) Changes in bone turnover in young women consuming different levels of dietary protein. J Clin Endocrinol Metab 84:1052–1055PubMedCrossRefGoogle Scholar
  20. Kraemer WJ, Volek JS, Bush JA, Putukian M, Sebastianelli WJ (1998) Hormonal responses to consecutive days of heavy-resistance exercise with or without nutritional supplementation. J Appl Physiol 85:1544–1555PubMedGoogle Scholar
  21. Kraemer WJ, Hakkinen K, Newton RU, Nindl BC, Volek JS, McCormick M, Gotshalk LA, Gordon SE, Fleck SJ, Campbell WW, Putukian M, Evans WJ (1999) Effects of heavy-resistance training on hormonal response patterns in younger vs. older men. J Appl Physiol 87:982–992Google Scholar
  22. Kraemer WJ, Ratamess NA, Volek JS, Hakkinen K, Rubin MR, French DN, Gomez AL, McGuigan MR, Scheett TP, Newton RU, Spiering BA, Izquierdo M, Dioguardi FS (2006) The effects of amino acid supplementation on hormonal responses to resistance training overreaching. Metabolism 55:282–291PubMedCrossRefGoogle Scholar
  23. Lukaski HC, Mendez J, Buskirk ER, Cohn SH (1981) Relationship between endogenous 3-methylhistidine excretion and body composition. Am J Physiol 240:E302–E307PubMedGoogle Scholar
  24. Mazzetti SA, Kraemer WJ, Volek JS, Duncan ND, Ratamess NA, Gomez AL (2000) The influence of direct supervision of resistance training on strength performance. Med Sci Sports Exerc 32:1175–1184PubMedCrossRefGoogle Scholar
  25. Pannemans DL, Schaafsma G, Westerterp KR (1997) Calcium excretion, apparent calcium absorption and calcium balance in young and elderly subjects: influence of protein intake. Br J Nutr 77:721–729PubMedCrossRefGoogle Scholar
  26. Phillips SM (2004) Protein requirements and supplementation in strength sports. J Nutr 20:689–695CrossRefGoogle Scholar
  27. Pinkoski C, Chilibeck PD, Candow DG, Esliger D, Ewaschuk JB, Facci M, Farthing JP, Zello GA (2006) The effects of conjugated linoleic acid supplementation during resistance training. Med Sci Sports Exerc 38:339–348PubMedCrossRefGoogle Scholar
  28. Pivarnik JM, Hickson JF, Wolinsky I (1989) Urinary 3-methylhistidine excretion increases with repeated weight training exercise. Med Sci Sports Exerc 21:283–286PubMedGoogle Scholar
  29. Rennie MJ, Millward DL (1983) 3-Methylhistidine excretion and urinary 3-methylhistidine/creatinine ratios are poor indicators of skeletal muscle protein breakdown. Clin Sci 65:217–225PubMedGoogle Scholar
  30. Rennie MJ, Edwards RH, Krywawych S, Davies CT, Halliday D, Waterlow JC, Millward DL (1981) Effect of exercise on protein turnover in man. Clin Sci 61:627–639PubMedGoogle Scholar
  31. Siri WE (1966) Body composition from fluid spaces and density: analysis of methods. In: Brozek J, Henschel A (eds) Techniques for measuring body composition. National Academy of Sciences National Research Council, Washington, pp 223–224Google Scholar
  32. Thomas S, Reading I, Shephard RJ (1992) Revision of the Physical Activity Readiness Questionnaire (PAR-Q). Can J Sport Sci 17:338–345PubMedGoogle Scholar
  33. Tipton KD, Rasmussen BB, Miller SL, Wolf SE, Owens-Stovall SK, Petrini BE, Wolfe RR (2001) Timing of amino acid–carbohydrate ingestion alters anabolic response of muscle to resistance exercise. Am J Physiol Endocrinol Metab 281:E197–E206PubMedGoogle Scholar
  34. Trappe T, Williams R, Carrithers J, Raue U, Esmarck B, Kjaer M, Hickner R (2004) Influence of age and resistance exercise on human skeletal muscle proteolysis: a microdialysis approach. J Physiol 554:803–813PubMedCrossRefGoogle Scholar
  35. Wassner SJ, Schlitzer JL, Li JB (1980) A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography. Anal Biochem 104:284–289PubMedCrossRefGoogle Scholar
  36. Welle S, Thornton CA (1998) High-protein meals do not enhance myofibrillar synthesis after resistance exercise in 62- to 75-yr-old men and women. Am J Physiol 274:E677–E683PubMedGoogle Scholar
  37. Welle S, Thornton C, Statt M (1995) Myofibrillar protein synthesis in young and old human subjects after three months of resistance training. Am J Physiol 268:E422–E427PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Darren G. Candow
    • 1
  • Philip D. Chilibeck
    • 2
  • Marina Facci
    • 3
  • Saman Abeysekara
    • 4
  • Gordon A. Zello
    • 4
  1. 1.School of Human KineticsLaurentian UniversitySudburyCanada
  2. 2.College of KinesiologyUniversity of Saskatchewan, 87 Campus DriveSaskatoonCanada
  3. 3.Department of Anatomy and Cell BiologyUniversity of SaskatchewanSaskatoonCanada
  4. 4.College of Pharmacy and NutritionUniversity of SaskatchewanSaskatoonCanada

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