European Journal of Applied Physiology

, Volume 97, Issue 4, pp 404–412 | Cite as

Acute resistance exercise does not change the hormonal response to sublingual androstenediol intake

Original Article


Sublingual intake of 21.4 mg androstenediol increases serum testosterone concentrations whereas swallowing 200 mg androstenediol does not. The duration of increase in serum testosterone following sublingual androstenediol (SL-DIOL) is unknown. Resistance exercise (EX) following SL-DIOL may cause larger increases in serum estradiol concentrations than while at rest. This project evaluated the duration of change in, and the effects of acute EX on, the hormonal response to SL-DIOL. Six young resistance trained males consumed either placebo (PL) or SL-DIOL before a single session of EX or no exercise (Rest) in a random, double blind, crossover manner (for a total of four trials). Blood samples were collected before supplementation, and at 60, 120, 180, 240, 480, and 720 min post-supplementation, with the exercise occurring between 60 and 120 min. The serum [total testosterone] increased (< 0.05) at 60 min similarly in SL-DIOL-EX and SL-DIOL-Rest by ∼115%, and at 120 min by ∼107% with no differences due to exercise. The serum [estradiol] increased (< 0.05) similarly in SL-DIOL-EX and SL-DIOL-Rest by ∼33% at 60 min and ∼45% at 120 min, with no differences due to exercise. Serum [testosterone] returned to baseline by 240 min and serum [estradiol] returned to baseline by 720 min post-intake. These findings indicate that SL-DIOL acutely elevates serum testosterone and estradiol concentrations, that EX does not alter the endocrine response to SL-DIOL, and that the increases in serum estradiol last between 480 and 720 min while the increases in serum testosterone last <240 min following acute SL-DIOL intake.


Androstenediol Testosterone Estradiol Androstenedione Resistance exercise 


  1. Summary of the second report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel II) (1993). JAMA 269:3015–3023Google Scholar
  2. Ballantyne CS, Phillips SM, MacDonald JR, Tarnopolsky MA, MacDougall JD (2000) The acute effects of androstenedione supplementation in healthy young males. Can J Appl Physiol 25:68–78PubMedGoogle Scholar
  3. Berkovitz GD, Carter KM, Brown TR, Migeon CJ (1990) Testosterone lowers aromatase activity in cultured human genital skin fibroblasts. Mol Cell Endocrinol 69:187–197PubMedCrossRefGoogle Scholar
  4. Bhasin S, Storer TW, Berman N, Callegari C, Clevenger B, Phillips J, et al (1996) The effects of supraphysiologic doses of testosterone on muscle size and strength in normal men. N Engl J Med 335:1–7PubMedCrossRefGoogle Scholar
  5. Blaquier J, Forchielli E, Dorfman RI (1967) In vitro metabolism of androgens in whole human blood. Acta Endocrinol (Copenh) 55:697–704Google Scholar
  6. Broeder CE, Quindry J, Brittingham K, Panton L, Thomson J, Appakondu S, et al (2000) The andro project: physiological and hormonal influences of androstenedione supplementation in men 35 to 65 years old participating in a high-intensity resistance training program. Arch Intern Med 160:3093–3104PubMedCrossRefGoogle Scholar
  7. Brown GA, Martini ER, Roberts BS, Vukovich MD, King DS (2002) Acute hormonal response to sublingual androstenediol intake in young men. J Appl Physiol 92:142–146PubMedGoogle Scholar
  8. Brown GA, Vukovich MD, Martini ER, Kohut ML, Franke WD, Jackson DA, et al (2000a) Endocrine responses to chronic androstenedione intake in 30- to 56-year-old men. J Clin Endocrinol Metab 85:4074–4080CrossRefGoogle Scholar
  9. Brown GA, Vukovich MD, Martini ER, Kohut ML, Franke WD, Jackson DA, et al (2001a) Effects of androstenedione-herbal supplementation on serum sex hormone concentrations in 30- to 59-year-old men. Int J Vitam Nutr Res 71:293–301CrossRefGoogle Scholar
  10. Brown GA, Vukovich MD, Martini ER, Kohut ML, Franke WD, Jackson DA, et al (2001b) Endocrine and lipid responses to chronic androstenediol-herbal supplementation in 30 to 58-year-old men. J Am Coll Nutr 20:520–528Google Scholar
  11. Brown GA, Vukovich MD, Reifenrath TA, Uhl NL, Parsons KA, Sharp RL, et al (2000b) Effects of anabolic precursors on serum testosterone concentrations and adaptations to resistance training in young men. Int J Sport Nutr Exerc Metab 10:340–359Google Scholar
  12. Brown GA, Vukovich MD, Sharp RL, Reifenrath TA, Parsons KA, King DS (1999) Effect of oral DHEA on serum testosterone and adaptations to resistance training in young men. J Appl Physiol 87:2274–2283PubMedGoogle Scholar
  13. Brown WJ, Basil MD, Bocarnea MC (2003) The influence of famous athletes on health beliefs and practices: Mark McGwire, child abuse prevention, and androstenedione. J Health Commun 8:41–57PubMedCrossRefGoogle Scholar
  14. Cadoux-Hudson TA, Few JD, Imms FJ (1985) The effect of exercise on the production and clearance of testosterone in well trained young men. Eur J Appl Physiol Occup Physiol 54:321–325PubMedCrossRefGoogle Scholar
  15. Delp MD (1999) Control of skeletal muscle perfusion at the onset of dynamic exercise. Med Sci Sports Exerc 31:1011–1018PubMedCrossRefGoogle Scholar
  16. Earnest CP, Olson MA, Broeder CE, Breuel KF, Beckham SG (2000) In vivo 4-androstene-3,17-dione and 4-androstene-3 beta,17 beta-diol supplementation in young men. Eur J Appl Physiol 81:229–232PubMedCrossRefGoogle Scholar
  17. Ferrando AA, Sheffield-Moore M, Yeckel CW, Gilkison C, Jiang J, Achacosa A, et al (2002) Testosterone administration to older men improves muscle function: molecular and physiological mechanisms. Am J Physiol Endocrinol Metab 282:E601–E607PubMedGoogle Scholar
  18. Frenkl R, Szeberenyi S (1976) Enzyme inducing effect of muscular exertion in the rat. Acta Med Acad Sci Hung 33:95–100PubMedGoogle Scholar
  19. Fyssas I, Syrigos KN, Konstandoulakis MM, Papadopoulos S, Milingos N, Anapliotou M, et al (1997) Sex hormone levels in the serum of patients with pancreatic adenocarcinoma. Horm Metab Res 29:115–118PubMedCrossRefGoogle Scholar
  20. Hoffman JR, Maresh CM, Armstrong LE, Gabaree CL, Bergeron MF, Kenefick RW, et al (1994) Effects of hydration state on plasma testosterone, cortisol and catecholamine concentrations before and during mild exercise at elevated temperature. Eur J Appl Physiol Occup Physiol 69:294–300PubMedCrossRefGoogle Scholar
  21. Horton R, Hsieh P, Barberia J, Pages L, Cosgrove M (1975) Altered blood androgens in elderly men with prostate hyperplasia. J Clin Endocrinol Metab 41:793–796PubMedGoogle Scholar
  22. Horton R, Tait JF (1966) Androstenedione production and interconversion rates measured in peripheral blood and studies on the possible site of its conversion to testosterone. J Clin Invest 45:301–313PubMedCrossRefGoogle Scholar
  23. Jasuja R, Ramaraj P, Mac RP, Singh AB, Storer TW, Artaza J, et al (2005) Delta-4-androstene-3,17-dione binds androgen receptor, promotes myogenesis in vitro, and increases serum testosterone levels, fat-free mass, and muscle strength in hypogonadal men. J Clin Endocrinol Metab 90:855–863PubMedCrossRefGoogle Scholar
  24. Kanayama G, Gruber AJ, Pope HG Jr, Borowiecki JJ, Hudson JI (2001) Over-the-counter drug use in gymnasiums: an underrecognized substance abuse problem? Psychother Psychosom 70:137–140PubMedCrossRefGoogle Scholar
  25. King DS, Sharp RL, Vukovich MD, Brown GA, Reifenrath TA, Uhl NL, et al (1999) Effect of oral androstenedione on serum testosterone and adaptations to resistance training in young men: a randomized controlled trial. JAMA 281:2020–2028PubMedCrossRefGoogle Scholar
  26. Kley HK, Deselaers T, Peerenboom H, Kruskemper HL (1980) Enhanced conversion of androstenedione to estrogens in obese males. J Clin Endocrinol Metab 51:1128–1132PubMedCrossRefGoogle Scholar
  27. Kraemer WJ (1988) Endocrine responses to resistance exercise. Med Sci Sports Exerc 20:S152–S157PubMedCrossRefGoogle Scholar
  28. Kraemer WJ, Marchitelli L, Gordon SE, Harman E, Dziados JE, Mello R, et al (1990) Hormonal and growth factor responses to heavy resistance exercise protocols. J Appl Physiol 69:1442–1450PubMedGoogle Scholar
  29. Kraemer WJ, Adams K, Cafarelli E, Dudley GA, Dooly C, Feigenbaum MS, et al (2002) American College of Sports Medicine position stand. Progression models in resistance training for healthy adults. Med Sci Sports Exerc 34:364–380PubMedCrossRefGoogle Scholar
  30. Labrie F, Belanger A, Cusan L, Candas B (1997) Physiological changes in dehydroepiandrosterone are not reflected by serum levels of active androgens and estrogens but of their metabolites: intracrinology. J Clin Endocrinol Metab 82:2403–2409PubMedCrossRefGoogle Scholar
  31. Leder BZ, Longcope C, Catlin DH, Ahrens B, Schoenfeld DA, Finkelstein JS (2000) Oral androstenedione administration and serum testosterone concentrations in young men. JAMA 283:779–782PubMedCrossRefGoogle Scholar
  32. Longcope C, Kato T, Horton R (1969) Conversion of blood androgens to estrogens in normal adult men and women. J Clin Invest 48:2191–2201PubMedCrossRefGoogle Scholar
  33. Longcope C, Pratt JH, Schneider SH, Fineberg SE (1978) Aromatization of androgens by muscle and adipose tissue in vivo. J Clin Endocrinol Metab 46:146–152PubMedGoogle Scholar
  34. Lundbergh P, Strandell T (1973) The effect of physical exercise on the wedged and free hepatic venous pressure in normal men. Acta Med Scand 194:211–214PubMedCrossRefGoogle Scholar
  35. Lupo C, Baldi L, Bonifazi M, Lodi L, Martelli G, Viti A, et al (1985) Androgen levels following a football match. Eur J Appl Physiol Occup Physiol 54:494–496PubMedCrossRefGoogle Scholar
  36. Mace AE (1974) Sample-size determination. Robert E. Krieger Publishing Company, Huntington, NY, pp 77–80Google Scholar
  37. Ploutz-Snyder LL, Convertino VA, Dudley GA (1995) Resistance exercise-induced fluid shifts: change in active muscle size and plasma volume. Am J Physiol 269:R536–R543PubMedGoogle Scholar
  38. Rasmussen BB, Volpi E, Gore DC, Wolfe RR (2000) Androstenedione does not stimulate muscle protein anabolism in young healthy men. J Clin Endocrinol Metab 85:55–59PubMedCrossRefGoogle Scholar
  39. Salehian B, Wang C, Alexander G, Davidson T, McDonald V, Berman N, et al (1995) Pharmacokinetics, bioefficacy, and safety of sublingual testosterone cyclodextrin in hypogonadal men: comparison to testosterone enanthate—a clinical research center study. J Clin Endocrinol Metab 80:3567–3575PubMedCrossRefGoogle Scholar
  40. Stuenkel CA, Dudley RE, Yen SS (1991) Sublingual administration of testosterone-hydroxypropyl-beta- cyclodextrin inclusion complex simulates episodic androgen release in hypogonadal men. J Clin Endocrinol Metab 72:1054–1059PubMedCrossRefGoogle Scholar
  41. Tremblay MS, Copeland JL, Van Helder W (2004) Effect of training status and exercise mode on endogenous steroid hormones in men. J Appl Physiol 96:531–539PubMedCrossRefGoogle Scholar
  42. Urban RJ, Bodenburg YH, Gilkison C, Foxworth J, Coggan AR, Wolfe RR, et al (1995) Testosterone administration to elderly men increases skeletal muscle strength and protein synthesis. Am J Physiol 269:E820–E826PubMedGoogle Scholar
  43. Villalpando S, Mondragon L, Barron C, Perez-Pasten E, Castaneda G, Alonso-Uriarte R, et al (1992) Role of testosterone and dihydrotestosterone in spontaneous gynecomastia of adolescents. Arch Androl 28:171–176PubMedCrossRefGoogle Scholar
  44. Volek JS, Gomez AL, Love DM, Avery NG, Sharman MJ, Kraemer WJ (2001) Effects of a high-fat diet on postabsorptive and postprandial testosterone responses to a fat-rich meal. Metabolism 50:1351–1355PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.HPERLS Department, Human Performance LaboratoryThe University of Nebraska at KearneyKearneyUSA
  2. 2.Jiann-Ping Hsu School of Public HealthGeorgia Southern UniversityStatesboroUSA

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