European Journal of Applied Physiology

, Volume 97, Issue 3, pp 261–271 | Cite as

Human skeletal muscle structure and function preserved by vibration muscle exercise following 55 days of bed rest

  • Dieter Blottner
  • Michele Salanova
  • Britta Püttmann
  • Gudrun Schiffl
  • Dieter Felsenberg
  • Björn Buehring
  • Jörn Rittweger
Original Article

Abstract

Prolonged immobilization of the human body results in functional impairments and musculoskeletal system deconditioning that may be attenuated by adequate muscle exercise. In a 56-day horizontal bed rest campaign involving voluntary males we investigated the effects of vibration muscle exercise (RVE, 2×6 min daily) on the lower limb skeletal muscles using a newly designed foot plantar trainer (Galileo Space) for use at supine position during bed rest. The maximally voluntary isometric plantar flexion force was maintained following regular RVE bouts during bed rest (controls −18.6 %, P<0.05). At the start (BR2) and end of bed rest (BR55) muscle biopsies were taken from both mixed fast/slow-type vastus lateralis (VL) and mainly slow-type soleus muscle (SOL), each having n=10. RVE group: the size of myofiber types I and II was largely unchanged in VL, and increased in SOL. Ctrl group: the SOL depicted a disrupted pattern of myofibers I/II profiles (i.e., type II>140 % vs. preBR) suggesting a slow-to-fast muscle phenotype shift. In RVE-trained SOL, however, an overall conserved myofiber I/II pattern was documented. RVE training increased the activity-dependent expression of nitric oxide synthase type 1 immunofluorescence at SOL and VL myofiber membranes. These data provide evidence for the beneficial effects of RVE training on the deconditioned structure and function of the lower limb skeletal muscle. Daily short RVE should be employed as an effective atrophy countermeasure co-protocol preferentially addressing postural calf muscles during prolonged clinical immobilization or long-term human space missions.

Keywords

Skeletal muscle atrophy Neuromuscular disorders Countermeasure Rehabilitation Spaceflight 

References

  1. Adamo DE, Martin BJ, Johnson PW (2002) Vibration-induced muscle fatigue, a possible contribution to musculoskeletal injury. Eur J Appl Physiol 88:134–140CrossRefPubMedGoogle Scholar
  2. Akima H, Kubo K, Kanehisa H, Suzuki Y, Gunji A, Fukunaga T (2000) Leg-press resistance training during 20 days of 6 degrees head-down tilt bed rest prevents muscle deconditioning. Eur J Physiol 82:30–38CrossRefGoogle Scholar
  3. Alkner BA, Tesch PA (2004) Knee extensor and plantar flexor muscle size and function in response to 90 d bed rest with or without resistance exercise. Eur J Appl Physiol 93:294–305CrossRefPubMedGoogle Scholar
  4. Baldwin KM, Haddad F (2001) Effects of different activities and inactivity on heavy chain gene expression in striated muscle. J Appl Physiol 90:345–357CrossRefPubMedGoogle Scholar
  5. Balon TW (1999) Integrative biology of nitric oxide and exercise. Exerc Sports Sci Rev 27:219–253CrossRefGoogle Scholar
  6. Berg HE, Tesch PA (1994) A gravity-independent ergometer to be used for resistance training in space. Aviat Space Environ Med 65:752–756PubMedGoogle Scholar
  7. Berg HE, Tesch PA (1998) Force and power characteristics of a resistance exercise device for use in space. Acta Astronaut 42:219–230CrossRefPubMedGoogle Scholar
  8. Bergström J (1962) Muscle electrolytes in man: determination by neutron activation analysis in needle biopsy specimens: a study on normal subjects, kidney patients, and patients with chronic diarrhea. Scand J Clin Lab Invest 14:1–110CrossRefGoogle Scholar
  9. Bleeker MW, De Groot PC, Rongen GA, Rittweger J, Felsenberg D, Smits P, Hopman MT (2005) Vascular adaptation to deconditioning and the effect of an exercise countermeasure: results of the Berlin Bed Rest study. J Appl Physiol 99:1923–1300CrossRefGoogle Scholar
  10. Blottner D, Lück G (2001) Just in time and place: NOS/NO system assembly in neuromuscular junction formation. Microsc Res Techn 55:171–180CrossRefGoogle Scholar
  11. Blottner D, Lück G (1998) Nitric oxide synthase (NOS) in mouse skeletal muscle development and differentiated myoblasts. Cell Tissue Res 292:293–302CrossRefPubMedGoogle Scholar
  12. Bongiovanni LG, Hagbarth KE, Stjernberg L (1990) Prolonged muscle vibration reducing motor output in maximally voluntary contracting in man. J Physiol 423:15–36PubMedGoogle Scholar
  13. Booth FW, Criswell DS (1997) Molecular events underlying skeletal muscle atrophy and the development of effective countermeasures. Int J Sports Med 18:265–269CrossRefGoogle Scholar
  14. Bredt D (1999) Endogenous nitric oxide synthesis: biological functions and pathophysiology. Free Radical Res 31:577–596CrossRefGoogle Scholar
  15. Caiozzo VJ, Haddad F, Baker MJ, Herrick RE, Prietto N, Baldwin KM (1996) Microgravity induced transformation of myosin isoforms and contractile proteins of skeletal muscle. J Appl Physiol 81:123–132PubMedGoogle Scholar
  16. Cardinale M, Bosco C (2003) The use of vibration as an exercise intervention. Exerc Sports Sci Rev 31:3–7CrossRefGoogle Scholar
  17. Cardinale M (2004) High-frequency vibration training able to increase muscle power in postmenopausal women. Arch Phys Med Rehabil 85:1854–1857CrossRefPubMedGoogle Scholar
  18. Carrithers JA, Tesch PA, Trieschmann J, Ekberg A, Trappe TA (2002) Skeletal muscle protein composition following 5 weeks of ULLS and resistance exercise. J Gravit Physiol 9:P155–P156PubMedGoogle Scholar
  19. Carroll JA, Carrithers JA, Trappe TA (2004) Contractile protein concentration in human single muscle fibers. J Muscle Res Cell Motil 25:55–59CrossRefPubMedGoogle Scholar
  20. Delecluse C, Roelants M, Verschueren S (2003) Strength increase after whole body vibration compared with resistance training. Med Sci Sports Exerc 35:1033–1041CrossRefPubMedGoogle Scholar
  21. Dietz V (2002) Proprioception and locomotor disorders. Nature Rev Neurosci 3:781–790CrossRefGoogle Scholar
  22. Dietz V, Duysens J (2000) Significance of load receptor input during locomotion: a review. Gait Posture 11:102–110CrossRefPubMedGoogle Scholar
  23. Dietz V, Horstmann GA, Trippel M, Gollhofer A (1989) Human postural reflexes and gravity—an underwater simulation. Neurosci Lett 106:350–355CrossRefPubMedGoogle Scholar
  24. Fitts RH, Riley DR, Widrick J (2001) Functional and structural adaptations of skeletal muscle to microgravity. J Exp Biol 204:3201–3208PubMedGoogle Scholar
  25. Gauthier GM, Roll JP, Martin BJ, Harley F (1981) Effects of whole body vibration on sensory motor system performance in man. Aviat Space Environ Med 52:473–479PubMedGoogle Scholar
  26. Griffin L, Garland SJ, Ivanova T, Gossen ER (2001) Muscle vibration sustains motor firing rate during submaximal isometric fatigue in humans. J Physiol 535:929–936CrossRefPubMedGoogle Scholar
  27. Harris JA, Benedict FG (1919) A biometric study of basal metabolism in man. Carnegie Institute of Washington, WashingtonGoogle Scholar
  28. Ivanenko YP, Grasso R, Lacquantini F (2000) Influence of leg muscle vibration on human walking. J Neurophysiol 84:1737–1747PubMedGoogle Scholar
  29. Martin BJ, Park HS (1997) Analysis of tonic vibration reflex: influence of vibration variables on motor unit synchronization and fatigue. Eur J Appl Physiol 75:504–511CrossRefGoogle Scholar
  30. Maffiuletti NA, Pensini M, Martin A (2002) Activation of human plantar flexor muscles increases after electrostimulation training. J Appl Physiol 92:1383–1392PubMedGoogle Scholar
  31. McBride JM, Porcari JP, Scheunke MD (2004) Effect of vibration during fatiguing resistance exercise on subsequent muscle activity during maximal voluntary isometric contractions. J Strength Cond Res 18:777–781CrossRefPubMedGoogle Scholar
  32. Meester J, Spitzenfeil P, Schwarzer J, Seifriz F (1999) Biological reaction to vibration-implications for sport. J Sci Med Sport 2:211–226CrossRefPubMedGoogle Scholar
  33. Ohira Y, Yoshinaga T, Ohara M, Nonaka I, Yoskioka T, Yamashita-Goto K, Shenkman BS, Kozlovskaya IB, Roy RR, Edgerton VR (1999) Myonuclear domain and myosin phenotype in human soleus after bed rest with or without loading. J Appl Physiol 87:1776–1785PubMedGoogle Scholar
  34. Park HS Martin BJ (1993) Contribution of the tonic vibration reflex to muscle stress and muscle fatigue. Scand J Work Environ Health 19:35–42PubMedGoogle Scholar
  35. Perkins WJ, Han Y-S, Sieck G (1997) Skeletal muscle force and actomyosin ATPase activity reduced by nitric oxide donor. J Appl Physiol 83:1326–1332PubMedGoogle Scholar
  36. Pette D, Staron RS (2001) Transitions of muscle fiber phenotype profiles. Histochem Cell Biol 115:359–372PubMedGoogle Scholar
  37. Rittweger J, Beller G. Felsenberg D (2000) Acute physiological effects of exhaustive whole body vibration exercise in man. Clin Physiol 20:134–142CrossRefPubMedGoogle Scholar
  38. Rittweger J, Mutschelknauss M, Felsenberg D (2003) Acute changes in neuromuscular excitability after exhaustive whole body vibration exercise compared to exhaustion by squatting exercise. Clin Physiol Funct Imaging 23:81–86CrossRefPubMedGoogle Scholar
  39. Roelants M, Delecluse C, Goris M, Verschueren S (2004) Effects of 24 weeks of whole body vibration training on body composition and muscle strength in untrained females. Int J Sports Med 25:1–5PubMedCrossRefGoogle Scholar
  40. Rubin C, Turner AS, Bain S, Mallinckrodt C, McLeod K (2001) Anabolism. Low mechanical signals strengthen long bones. Nature 412:603–604CrossRefPubMedGoogle Scholar
  41. Rudnick J, Püttmann B, Tesch PA, Alkner B, Schoser BG, Salanova M, Kirsch K, Gunga HC, Schiffl G, Lück G, Blottner D (2004) Differential expression of nitric oxide synthases (NOS 1–3) in human skeletal muscle following exercise countermeasure during 12 weeks of bed rest. FASEB J 18:1228–30PubMedGoogle Scholar
  42. Roy RR, Zhong H, Monti RJ, Vallance KA, Kim JA, Edgerton VR (2000) Mechanical properties and fiber type composition of chronically inactive muscles. J Gravit Physiol 7:P103–104PubMedGoogle Scholar
  43. Sale DG (1988) Neural adaptation to resistance training. Med Sci Sports Exerc 20:S135–S145PubMedCrossRefGoogle Scholar
  44. Schuler M, Pette D (1996) Fiber transformation and replacement in low-frequency stimulated rabbit fast-twitch muscles. Cell Tissue Res 285:297–303CrossRefPubMedGoogle Scholar
  45. Shakleford LC, LeBlanc AD, Driscoll TB, Evans HJ, Rianon NJ, Smith SM, Spector E, Feeback DL, Lai D (2004) Resistance exercise as a countermeasure to disuse-induced bone loss. J Appl Physiol 97:119–129CrossRefPubMedGoogle Scholar
  46. Trappe TA, Raue U, Tesch PA (2004) Human soleus muscle protein synthesis following resistance exercise. Acta Physiol Scand 182:189–196CrossRefPubMedGoogle Scholar
  47. Verschueren SMP, Swinnen SP, Desloovere K, Duysen J (2002) Effects of tendon vibration on the spatiotemporal characteristics of human locomotion. Exp Brain Res 143:231–239CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Dieter Blottner
    • 1
  • Michele Salanova
    • 1
  • Britta Püttmann
    • 1
  • Gudrun Schiffl
    • 1
  • Dieter Felsenberg
    • 2
  • Björn Buehring
    • 2
  • Jörn Rittweger
    • 3
  1. 1.Department of Vegetative Anatomy, Center of Space Medicine Berlin, Neuromuscular GroupCharité University Medicine Berlin, Campus Benjamin FranklinBerlinGermany
  2. 2.Center for Muscle and Bone Research (ZMK)Charité Universitätsmedizin Berlin, Campus Benjamin FranklinBerlinGermany
  3. 3.Institute for Biophysical and Clinical Research into Human Movement Manchester Metropolitan University AlsagerUK

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