Seven days’ around the clock exhaustive physical exertion combined with energy depletion and sleep deprivation primes circulating leukocytes
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Both exhaustive physical exertion and starvation have been reported to induce depression of immune function. The aim of the present study was to investigate the inflammatory environment and state of activation and mediator-producing potential of circulating leukocytes during prolonged physical activity with concomitant energy and sleep deprivation. Eight well-trained males were studied during 7 days of semi-continuous physical activity. Sleep was restricted to about 1 h/24 h, energy intake to 1.5– 3.0 MJ/24 h. Blood was drawn at 07.00 a.m. on days 0, 2, 4, and 7. Plasma levels of inflammation markers were measured. The response of circulating leukocytes to lipopolysaccharide (LPS; 1 μg mL−1), and the effect of added hydrocortisone (10 and 100 nmol L−1), were measured in the supernatant after 3 h of incubation in an ex vivo whole blood model. Activation of leukocytes steadily increased as measured by plasma matrix metalloproteinase-9, tumour necrosis factor-α, interleukin-1β, and interleukin-6. Inhibitors of systemic inflammation were either unaltered (tissue inhibitor of matrix metalloproteinase-1) or elevated (plasma interleukin-1 receptor antagonist). Cortisol levels increased on days 2 and 4, but thereafter reverted to baseline values. The leukocytes responded to LPS activation with increasing release of inflammatory cytokines throughout the study period. The anti-inflammatory potency of hydrocortisone decreased. Prolonged multifactorial stress thus activated circulating immune cells and primed them for an increased response to a subsequent microbial challenge.
KeywordsExercise Inflammation Glucocorticoids Leukocytes Stress
We thank the cadets at the Norwegian Military Academy who volunteered to take part in this study and thus were exposed to additional strain.
- Eversen CA, Toth LA (2000) Systemic bacterial invasion induced by sleep deprivation. Am J Physiol 278:R905–R916Google Scholar
- Fellmann N (1992) Hormonal and plasma volume alterations following endurance exercise. A brief review. Sports Med 13:37–49Google Scholar
- Goldberg AC, Eliaschewitz FG, Montor WR, Baracho GV, Errante PR, Callero MA, Cardoso MR, Braga PE, Kalil J, Sogayar MC, Rizzo LV (2005) Exogenous leptin restores in vitro T-cell proliferation and cytokine synthesis in patients with common variable immunodeficiency syndrome. Clin Immunol 114:147–153PubMedCrossRefGoogle Scholar
- Marable NL, Hickson JF, Korslund MK, Herbert WG, Desjardins RF, Thye FW (1979) Urinary nitrogen excretion as influenced by muscle building. Exercise program and protein intake variation. Nutr Rep Int 19:795–805Google Scholar
- Opstad PK (1995) Medical consequences in young men of prolonged physical stress with sleep and energy deficiency. Norwegian Defence Research Establishment /Publication-95/05586, Kjeller, NorwayGoogle Scholar