European Journal of Applied Physiology

, Volume 94, Issue 4, pp 442–447 | Cite as

Effect of skin temperature on the ion reabsorption capacity of sweat glands during exercise in humans

  • A. K. M. Shamsuddin
  • T. Kuwahara
  • A. Oue
  • C. Nomura
  • S. Koga
  • Y. Inoue
  • N. Kondo
Original Article


The effect of skin temperature on the ion reabsorption capacity of sweat glands during exercise in humans is unknown. In this study, eight healthy subjects performed a 60-min cycling exercise at a constant intensity (60% VO2max) under moderate (25°C) and cool (15°C) ambient temperatures at a constant relative humidity of 40%. The sweating rate (SR), index of sweat ion concentration (ISIC) by using sweat conductivity, esophageal temperature (Tes), mean skin temperature, and heart rate (HR) were measured continuously under both ambient temperatures. The SR and ISIC were significantly lower at the cool ambient temperature versus the moderate temperature. There were no significant differences in the changes in HR and esophageal temperature between these ambient temperature conditions, while the mean skin temperature was significantly lower at the cool ambient temperature by almost 3°C (P<0.05). The slopes of the relationships between Tes and the SR and ISIC were significantly lower and the thresholds of these relationships were significantly higher at the cool ambient temperature (P<0.05). The ion reabsorption capacity of the sweat glands was significantly lower (P<0.05) in a cool environment (0.21±0.04 vs. 0.52±0.06 mg/cm2/min at 15 and 25°C, respectively) as evaluated using the relationships for SR and ISIC. The results suggest that the ion reabsorption capacity of the sweat glands is influenced by skin temperature during exercise in humans.


Sweat ion concentration Ion reabsorption capacity In vivo Thermoregulation 



We sincerely thank our volunteer subjects. This study was supported by a grant-in-aid from the Japan Society for the Promotion of Science in the form of a post-doctoral fellowship from the Ministry of Education, Culture, Sport, Science and Technology, Japan (P01344).


  1. Allan JR, Wilson CG (1971) Influence of acclimatization on sweat sodium concentration. J Appl Physiol 30:708–712Google Scholar
  2. Allsopp AJ, Sutherland R, Wood P, Wootton SA (1998) The effect of sodium balance on sweat sodium secretion and plasma aldosterone concentration. Eur J Appl Physiol 78:516–521Google Scholar
  3. Bulmer MG, Forwell GD (1956) The concentration of sodium in thermal sweat. J Physiol (Lond) 132:115–122Google Scholar
  4. Cage G, Dobson RL (1965) Sodium secretion and reabsorption in the human sweat gland. J Clin Invest 44:1270–1276Google Scholar
  5. Chraïbi A, Horisberger JD (2002) Na self-inhibition of human epithelial Na channel: temperature dependence and effect of extracellular proteases. J Gen Physiol 120:133–145Google Scholar
  6. Chraïbi A, Horisberger JD (2003) Dual effect of temperature on the human epithelial Na+ channel. Pflugers Arch Eur J Physiol 447:316–320Google Scholar
  7. Falk B, Bar OR, MacDougall JD (1991) Aldosterone and prolactin response to exercise in the heat in circumpubertal boys. J Appl Physiol 71:1741–1745Google Scholar
  8. Francois V (1999) Early aldosterone action: toward filling the gap between transcription and transport. Am J Physiol 277 (Renal Physiol 46), F319–F327Google Scholar
  9. Hardy JD, DuBois EF (1938) The technique of measuring radiation and convection. J Nutr 15:461–475Google Scholar
  10. Johnson RE, Pitts GC, Consolazio FC (1944) Factors influencing chloride concentration in human sweat. Am J Physiol 141:575–589Google Scholar
  11. Kirby CR, Convertino VA (1986) Plasma aldosterone and sweat sodium concentrations after exercise and heat acclimatization. J Appl Physiol 61:967–970Google Scholar
  12. Kondo N, Shibasaki M, Aoki K, Koga S, Inoue Y, Crandall CG (2001) The function of human eccrine sweat gland during passive heat stress and dynamic exercise. J Appl Physiol 90:1877–1881Google Scholar
  13. Kuno Y (1956) Human perspiration. Thomas, SpringfieldGoogle Scholar
  14. Melin B, Eclache JP, Geelen G, Annat G, Allevard AM, Jarsaillon E, Zebidi A, Legros JJ, Gharib C (1980) Plasma AVP, neurophysin, renin activity, and aldosterone during submaximal exercise performed until exhaustion in trained and untrained men. Eur J Appl Physiol Occup Physiol 44:141–151Google Scholar
  15. Mizushima T, Tajima F, Okawa H, Umezu Y, Furusawa K, Ogata H (2003) Cardiovascular and endocrine responses during the cold pressor test in subjects with spinal cord injuries. Arch Phys Med Rehabil 84:112–118Google Scholar
  16. Nadel ER, Bullard RW, Stolwijk JAJ (1971) Importance of skin temperature in regulation of sweating. J Appl Physiol 31:80–87Google Scholar
  17. Ogawa T, Sugenoya J (1993) Pulsatile sweating and sympathetic sudomotor activity. Jpn J Physiol 43:275–289Google Scholar
  18. Orenstein DM, Henke KG, Green CG (1984) Heat acclimation in cystic fibrosis. J Appl Physiol Respirat Environ Exerc Physiol 57:408–412Google Scholar
  19. Pääkkönen T, Leppäluoto J (2002) Cold exposure and hormonal secretion: a review. Int J Circumpolar Health 61:265–276Google Scholar
  20. Robinson SID, Gerking SD, Turrell ES, Kincaid RK (1950) Effect of skin temperature on salt concentration of sweat. J Appl Physiol 2:654–662Google Scholar
  21. Ruff RL (1999) Effects of temperature on slow and fast inactivation of rat skeletal muscle Na+ channels. Am J Physiol 277 (Cell Physiol 46), C937–C947Google Scholar
  22. Sato K (1977) The physiology, pharmacology, and biochemistry of the eccrine sweat gland. Rev Physiol Biochem Pharmacol 79:51–131Google Scholar
  23. Sato K, Kang WH, Saga K, Sato KT (1989) Biology of sweat glands and their disorders. I. Normal sweat gland function. J Am Acad Dermatol 20:713–726Google Scholar
  24. Schwartz IL, Thaysen JH (1956) Excretion of sodium and potassium in human sweat. J Clin Invest 34:114–120Google Scholar
  25. Shamsuddin AKM, Togawa T (1998) Continuous monitoring of sweating by electrical conductivity measurement. Physiol Meas 19:375–382Google Scholar
  26. Shamsuddin AKM, Togawa T (2000) Continuous monitoring of single-sweat-gland activity. Physiol Meas 21:535–540Google Scholar
  27. Shamsuddin AKM, Yanagimoto S, Kuwahara T, Zhang Y, Nomura C, Kondo N (2005) Changes in the index of sweat ion concentration with increasing sweat during passive heat stress in humans. Eur J Appl Physiol (in press)Google Scholar
  28. Sugenoya J, Iwase S, Mano T, Ogawa T (1990) Identification of sudomotor activity in cutaneous sympathetic nerves using sweat expulsion as the effector response. Eur J Appl Physiol Occup Physiol 61:302–308Google Scholar
  29. Weiner JS, Heyningen RE (1952) Relation of skin temperature to salt concentration of general body sweat. J Appl Physiol 4:725–733Google Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • A. K. M. Shamsuddin
    • 1
  • T. Kuwahara
    • 1
  • A. Oue
    • 1
  • C. Nomura
    • 1
  • S. Koga
    • 2
  • Y. Inoue
    • 3
  • N. Kondo
    • 1
  1. 1.Laboratory for Applied Human Physiology, Faculty of Human DevelopmentKobe UniversityKobeJapan
  2. 2.Kobe Design UniversityKobeJapan
  3. 3.Osaka International UniversityOsakaJapan

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