European Journal of Applied Physiology

, Volume 96, Issue 5, pp 558–563 | Cite as

Effects of pedal frequency on estimated muscle microvascular O2 extraction

  • Leonardo F. Ferreira
  • Barbara J. Lutjemeier
  • Dana K. Townsend
  • Thomas J. Barstow
Original Article


An increase in muscle contraction frequency could limit muscle blood flow \( {\left( {\ifmmode\expandafter\dot\else\expandafter\.\fi{Q}_{{\text{M}}} } \right)}, \) compromising the matching of \( \ifmmode\expandafter\dot\else\expandafter\.\fi{Q}_{{\text{M}}} \) and muscle oxygen uptake \( {\left( {\ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2M}}}} }} } \right)}. \) This study examined the effects of pedal cadence on skeletal muscle oxygenation at low, moderate and peak exercise. Nine healthy subjects [24.7±6.3 years (SD)] performed incremental cycling exercise at 60 and 100 rpm. Pulmonary \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{\text{2}}} }} {\left( {\ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2P}}}} }} } \right)} \) was measured breath-by-breath and vastus lateralis oxygenation was determined by near-infrared spectroscopy (NIRS). The deoxyhemoglobin signal ([HHb]) from NIRS was used to estimate microvascular O2 extraction (i.e., [HHb] ∝ \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2M}}}} }} {\text{/}}\ifmmode\expandafter\dot\else\expandafter\.\fi{Q}_{{\text{M}}} \)). The \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2P}}}} }} \) and [HHb] for low, moderate and at peak exercise were determined. The \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2P}}}} }} \) at 60 rpm (low=0.64±0.13, moderate=2.03±0.38 and peak=3.39±0.84 l/min) were lower (P<0.01) than at 100 rpm (1.29±0.23, 2.14±0.39 and 3.54±0.88 l/min, respectively). There was a progressive increase in [HHb] from low to peak exercise. However, there was no significant difference (ANOVA, P=0.94) for the 60 (in μM, low=24.0±9.5, moderate=30.5±13.8 and peak=36.7±16.5) and 100 contractions/min (in μM, low=25.7±11.6, moderate=32.1±14.0 and peak=35.4±16.5). We conclude that vastus lateralis O2 extraction was similar at 60 and 100 cpm, suggesting that the \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2M}}}} }} {\text{/}}\ifmmode\expandafter\dot\else\expandafter\.\fi{Q}_{{\text{M}}} \) in the microcirculation was not altered and, presumably, no impairment of \( \ifmmode\expandafter\dot\else\expandafter\.\fi{Q}_{{\text{M}}} \) occurred with the increase in pedal frequency.


Exercise Blood flow Contraction frequency Muscle oxygenation Near-infrared spectroscopy 



This work was supported in part by American Heart Association, Grant-in-Aid # 0151183Z to T.J. Barstow. L.F. Ferreira was supported by a Fellowship from the Ministry of Education/CAPES - Brazil.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Leonardo F. Ferreira
    • 1
  • Barbara J. Lutjemeier
    • 1
  • Dana K. Townsend
    • 1
  • Thomas J. Barstow
    • 1
    • 2
  1. 1.Departments of Kinesiology and Anatomy and PhysiologyKansas State UniversityManhattanUSA
  2. 2.Department of KinesiologyKansas State UniversityManhattanUSA

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