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European Journal of Applied Physiology

, Volume 96, Issue 5, pp 558–563 | Cite as

Effects of pedal frequency on estimated muscle microvascular O2 extraction

  • Leonardo F. Ferreira
  • Barbara J. Lutjemeier
  • Dana K. Townsend
  • Thomas J. Barstow
Original Article

Abstract

An increase in muscle contraction frequency could limit muscle blood flow \( {\left( {\ifmmode\expandafter\dot\else\expandafter\.\fi{Q}_{{\text{M}}} } \right)}, \) compromising the matching of \( \ifmmode\expandafter\dot\else\expandafter\.\fi{Q}_{{\text{M}}} \) and muscle oxygen uptake \( {\left( {\ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2M}}}} }} } \right)}. \) This study examined the effects of pedal cadence on skeletal muscle oxygenation at low, moderate and peak exercise. Nine healthy subjects [24.7±6.3 years (SD)] performed incremental cycling exercise at 60 and 100 rpm. Pulmonary \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{\text{2}}} }} {\left( {\ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2P}}}} }} } \right)} \) was measured breath-by-breath and vastus lateralis oxygenation was determined by near-infrared spectroscopy (NIRS). The deoxyhemoglobin signal ([HHb]) from NIRS was used to estimate microvascular O2 extraction (i.e., [HHb] ∝ \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2M}}}} }} {\text{/}}\ifmmode\expandafter\dot\else\expandafter\.\fi{Q}_{{\text{M}}} \)). The \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2P}}}} }} \) and [HHb] for low, moderate and at peak exercise were determined. The \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2P}}}} }} \) at 60 rpm (low=0.64±0.13, moderate=2.03±0.38 and peak=3.39±0.84 l/min) were lower (P<0.01) than at 100 rpm (1.29±0.23, 2.14±0.39 and 3.54±0.88 l/min, respectively). There was a progressive increase in [HHb] from low to peak exercise. However, there was no significant difference (ANOVA, P=0.94) for the 60 (in μM, low=24.0±9.5, moderate=30.5±13.8 and peak=36.7±16.5) and 100 contractions/min (in μM, low=25.7±11.6, moderate=32.1±14.0 and peak=35.4±16.5). We conclude that vastus lateralis O2 extraction was similar at 60 and 100 cpm, suggesting that the \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{{\text{2M}}}} }} {\text{/}}\ifmmode\expandafter\dot\else\expandafter\.\fi{Q}_{{\text{M}}} \) in the microcirculation was not altered and, presumably, no impairment of \( \ifmmode\expandafter\dot\else\expandafter\.\fi{Q}_{{\text{M}}} \) occurred with the increase in pedal frequency.

Keywords

Exercise Blood flow Contraction frequency Muscle oxygenation Near-infrared spectroscopy 

Notes

Acknowledgments

This work was supported in part by American Heart Association, Grant-in-Aid # 0151183Z to T.J. Barstow. L.F. Ferreira was supported by a Fellowship from the Ministry of Education/CAPES - Brazil.

References

  1. Andersen P, Saltin B (1985) Maximal perfusion of skeletal muscle in man. J Physiol 366:233–249PubMedGoogle Scholar
  2. Beaver WL, Wasserman K, Whipp BJ (1986) A new method for detecting anaerobic threshold by gas exchange. J Appl Physiol 60:2020–2027PubMedGoogle Scholar
  3. Bellemare F, Wight D, Lavigne CM, Grassino A (1983) Effect of tension and timing of contraction on the blood flow of the diaphragm. J Appl Physiol 54:1597–1606PubMedGoogle Scholar
  4. Buchler B, Magder S, Roussos C (1985) Effects of contraction frequency and duty cycle on diaphragmatic blood flow. J Appl Physiol 58:265–273PubMedGoogle Scholar
  5. Cardus J, Marrades RM, Roca J, Barbera JA, Diaz O, Masclans JR, Rodriguez-Roisin R, Wagner PD (1998) Effects of FIO2 on leg \({\ifmmode\expandafter\dot\else\expandafter\.\fi{V}}_{{\text{O}}_{{\text{2}}}} \) during cycle ergometry in sedentary subjects. Med Sci Sports Exerc 30:697–703Google Scholar
  6. Di Prampero PE (2003) Factors limiting maximal performance in humans. Eur J Appl Physiol 90:420–429PubMedCrossRefGoogle Scholar
  7. Ferguson RA, Ball D, Krustrup P, Aagaard P, Kjaer M, Sargeant AJ, Hellsten Y, Bangsbo J (2001) Muscle oxygen uptake and energy turnover during dynamic exercise at different contraction frequencies in humans. J Physiol 536:261–271CrossRefPubMedGoogle Scholar
  8. Ferrari M, Binzoni T, Quaresima V (1997) Oxidative metabolism in muscle. Philos Trans R Soc Lond B Biol Sci 352:677–683PubMedCrossRefGoogle Scholar
  9. Ferrari M, Mottola L, Quaresima V (2004) Principles, techniques, and limitations of near infrared spectroscopy. Can J Appl Physiol 29:463–487PubMedGoogle Scholar
  10. Ferreira LF, Townsend DK, Lutjemeier BJ, Barstow TJ (2005) Muscle capillary blood flow kinetics estimated from pulmonary O2 uptake and near-infrared spectroscopy. J Appl Physiol 98:1820–1828CrossRefPubMedGoogle Scholar
  11. Gotshall RW, Bauer TA, Fahrner SL (1996) Cycling cadence alters exercise hemodynamics. Int J Sports Med 17:17–21PubMedCrossRefGoogle Scholar
  12. Grassi B, Pogliaghi S, Rampichini S, Quaresima V, Ferrari M, Marconi C, Cerretelli P (2003) Muscle oxygenation and pulmonary gas exchange kinetics during cycling exercise on-transitions in humans. J Appl Physiol 95:149–158PubMedGoogle Scholar
  13. Hamann JJ, Buckwalter JB, Clifford PS (2004) Vasodilatation is obligatory for contraction-induced hyperaemia in canine skeletal muscle. J Physiol 557:1013–1020CrossRefPubMedGoogle Scholar
  14. Hoelting BD, Scheuermann BW, Barstow TJ (2001) Effect of contraction frequency on leg blood flow during knee extension exercise in humans. J Appl Physiol 91:671–679PubMedGoogle Scholar
  15. Jones AM, Campbell IT, Pringle JS (2004) Influence of muscle fibre type and pedal rate on the \({\ifmmode\expandafter\dot\else\expandafter\.\fi{V}}_{{\text{O}}_{{\text{2}}}} \)-work rate slope during ramp exercise. Eur J Appl Physiol 91:238–245Google Scholar
  16. Knight DR, Poole DC, Schaffartzik W, Guy HJ, Prediletto R, Hogan MC, Wagner PD (1992) Relationship between body and leg \({\ifmmode\expandafter\dot\else\expandafter\.\fi{V}}_{{\text{O}}_{{\text{2}}}} \) during maximal cycle ergometry. J Appl Physiol 73:1114–1121Google Scholar
  17. Knight DR, Schaffartzik W, Poole DC, Hogan MC, Bebout DE, Wagner PD (1993) Effects of hyperoxia on maximal leg O2 supply and utilization in men. J Appl Physiol 75:2586–2594PubMedGoogle Scholar
  18. Kowalchuk JM, Rossiter HB, Ward SA, Whipp BJ (2002) The effect of resistive breathing on leg muscle oxygenation using near-infrared spectroscopy during exercise in men. Exp Physiol 87:601–611CrossRefPubMedGoogle Scholar
  19. Li L, Caldwell GE (1998) Muscle coordination in cycling: effect of surface incline and posture. J Appl Physiol 85:927–934PubMedGoogle Scholar
  20. Lutjemeier BJ, Miura A, Scheuermann BW, Koga S, Townsend DK, Barstow TJ (2005) Muscle contraction-blood flow interactions during upright knee extension exercise in humans. J Appl Physiol 98:1575–1583CrossRefPubMedGoogle Scholar
  21. MacIntosh BR, Neptune RR, Horton JF (2000) Cadence, power, and muscle activation in cycle ergometry. Med Sci Sports Exerc 32:1281–1287CrossRefPubMedGoogle Scholar
  22. McDaniel J, Durstine JL, Hand GA, Martin JC (2002) Determinants of metabolic cost during submaximal cycling. J Appl Physiol 93:823–828PubMedGoogle Scholar
  23. Osada T, Radegran G (2002) Femoral artery inflow in relation to external and total work rate at different knee extensor contraction rates. J Appl Physiol 92:1325–1330PubMedGoogle Scholar
  24. Poole DC, Wagner PD, Wilson DF (1995) Diaphragm microvascular plasma PO2 measured in vivo. J Appl Physiol 79:2050–2057PubMedGoogle Scholar
  25. Quaresima V, Homma S, Azuma K, Shimizu S, Chiarotti F, Ferrari M, Kagaya A (2001) Calf and shin muscle oxygenation patterns and femoral artery blood flow during dynamic plantar flexion exercise in humans. Eur J Appl Physiol 84:387–394CrossRefPubMedGoogle Scholar
  26. Richardson RS, Poole DC, Knight DR, Kurdak SS, Hogan MC, Grassi B, Johnson EC, Kendrick KF, Erickson BK, Wagner PD (1993) High muscle blood flow in man: is maximal O2 extraction compromised? J Appl Physiol 75:1911–1916PubMedGoogle Scholar
  27. Richardson RS, Noyszewski EA, Kendrick KF, Leigh JS, Wagner PD (1995) Myoglobin O2 desaturation during exercise. Evidence of limited O2 transport. J Clin Invest 96:1916–1926PubMedCrossRefGoogle Scholar
  28. Sheriff DD, Hakeman AL (2001) Role of speed vs. grade in relation to muscle pump function at locomotion onset. J Appl Physiol 91:269–276PubMedGoogle Scholar
  29. Sjogaard G, Hansen EA, Osada T (2002) Blood flow and oxygen uptake increase with total power during five different knee-extension contraction rates. J Appl Physiol 93:1676–1684PubMedGoogle Scholar
  30. Takaishi T, Ishida K, Katayama K, Yamazaki K, Yamamoto T, Moritani T (2002) Effect of cycling experience and pedal cadence on the near-infrared spectroscopy parameters. Med Sci Sports Exerc 34:2062–2071CrossRefPubMedGoogle Scholar
  31. Valic Z, Buckwalter JB, Clifford PS (2005) Muscle blood flow response to contraction: influence of venous pressure. J Appl Physiol 98:72–76CrossRefPubMedGoogle Scholar
  32. Walloe L, Wesche J (1988) Time course and magnitude of blood flow changes in the human quadriceps muscles during and following rhythmic exercise. J Physiol 405:257–273PubMedGoogle Scholar
  33. Wasserman K, Whipp BJ, Koyl SN, Beaver WL (1973) Anaerobic threshold and respiratory gas exchange during exercise. J Appl Physiol 35:236–243PubMedGoogle Scholar
  34. Whipp BJ (1994) The bioenergetic and gas exchange basis of exercise testing. Clin Chest Med 15:173–192PubMedGoogle Scholar
  35. Zoladz JA, Rademaker AC, Sargeant AJ (1995) Non-linear relationship between O2 uptake and power output at high intensities of exercise in humans. J Physiol 488:211–217PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Leonardo F. Ferreira
    • 1
  • Barbara J. Lutjemeier
    • 1
  • Dana K. Townsend
    • 1
  • Thomas J. Barstow
    • 1
    • 2
  1. 1.Departments of Kinesiology and Anatomy and PhysiologyKansas State UniversityManhattanUSA
  2. 2.Department of KinesiologyKansas State UniversityManhattanUSA

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