European Journal of Applied Physiology

, Volume 96, Issue 6, pp 636–643

The effects of short-term sprint training on MCT expression in moderately endurance-trained runners

  • Dale C. Bickham
  • David J. Bentley
  • Peter F. Le Rossignol
  • David Cameron-Smith
Original Articles


The purpose of this study was to assess the effects of short-term sprint training on transient changes in monocarboxylate lactate transporter 1 (MCT1) and MCT4 protein and mRNA content. Seven moderately endurance-trained runners (mean ± SE; age 27.7±2.9 years, body mass 81.1±5.9 kg, \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}{\text{O}}_{2} \;\max \) 58.1±2.0 ml kg−1 min−1) completed a \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}{\text{O}}_{2} \;\max \) and a supramaximal running test to exhaustion (RTE) before and after a 6-week period of sprint training. The sprint training was progressive and consisted of 18 sessions of near maximal short duration (5–15 s) sprints to compliment the athlete’s endurance training. Prior to the training period there was a significant (P<0.05) increase in MCT1, but not MCT4 protein, 2 h after the RTE. This occurred without any change in corresponding mRNA levels. After the training period, there was a significant increase in MCT1 protein but no significant change in the MCT4 isoform. Both MCT1 and MCT4 mRNA was significantly lower at rest and 2 h post-RTE after the completion of the training period. After the training period, there was a significant increase in the time to exhaustion and distance covered during the RTE. This study demonstrates that sprint training of this length and type results in an upregulation of MCT1 protein, but not MCT4 content. Additionally, this study shows conflicting adaptations in MCT1 and MCT4 protein and mRNA levels following training, which may indicate post-transcriptional regulation of MCT expression in human muscle.


Anaerobic Running Fiber type Interval Lactate transport 


  1. Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W, Lipman D (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402CrossRefPubMedGoogle Scholar
  2. Bonen A (2001) The expression of lactate transporters (MCT1 and MCT4) in heart and muscle. Eur J Appl Physiol 86:6–11PubMedCrossRefGoogle Scholar
  3. Bonen A, McCullagh KJA, Putman CT, Hultman E, Jones NL, Heigenhauser GJF (1998) Short-term training increases human muscle MCT1 and femoral venous lactate in relation to muscle lactate. Am J Physiol 274:E102–E107PubMedGoogle Scholar
  4. Bonen A, Tonouchi M, Miskovic D, Heddle C, Heikkila JJ, Halestrap A (2000) Isoform-specific regulation of the lactate transporters MCT1 and MCT4 by contractile activity. Am J Physiol 279:E1131– E1138Google Scholar
  5. Brooks GA, Brown MA, Butz CA, Sicurello JP, DuBouchaud H (1999) Cardiac and skeletal muscle mitochondria have a monocarboxylate transporter MCT1. J Appl Physiol 87:1713–1718PubMedGoogle Scholar
  6. Buck D, McNaughton LR (1999) Changing the number of submaximal exercise bouts effects calculation of MAOD. Int J Sports Med 20:28–33PubMedCrossRefGoogle Scholar
  7. Coles L, Litt J, Hatta H, Bonen A (2004) Exercise rapidly increases expression of the monocarboxylate transporters MCT1 and MCT4 in rat muscle. J Physiol 561:253–261CrossRefPubMedGoogle Scholar
  8. Dawson B, Fitzsimons M, Green S, Goodman C, Carey M, Cole K (1998) Changes in performance, muscle metabolites, enzymes and fiber types after short sprint training. Eur J Appl Physiol 78:163–169CrossRefGoogle Scholar
  9. DuBouchaud H, Butterfield GE, Wolfel EE, Bergman BC, Brooks GA (2000) Endurance training, expression, and physiology if LDH, MCT1, and MCT4 in human skeletal muscle. Am J Physiol 278:E571–E579PubMedGoogle Scholar
  10. Evertsen F, Medbø J, Bonen A (2001) Effect of training intensity on muscle lactate transporters and lactate threshold of cross-country skiers. Acta Physiol Scand 173:195–205CrossRefPubMedGoogle Scholar
  11. Garcia CK, Goldstein JL, Pathak RK, Anderson G, Brown M (1994) Molecular characterization of a membrane transporter for lactate, pyruvate, and other monocarboxylates: implications for the Cori cycle. Cell 76:865–873CrossRefPubMedGoogle Scholar
  12. Green H, Halestrap A, Mockett C, O’Toole D, Grant S, Ouyang J (2002) Increases in muscle MCT are associated with reductions in muscle lactate after a single exercise session in humans. Am J Physiol 282:E154–E160Google Scholar
  13. Halestrap AP, Price NT (1999) The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation. Biochem J 343:281–299CrossRefPubMedGoogle Scholar
  14. Hatta H, Tonouchi M, Miskovic D, Wang Y, Heikalla JJ, Bonen A (2001) Tissue specific and isoform-specific changes in MCT1 and MCT4 in heart and soleus muscle during a 1-yr period. Am J Physiol 281:E749–E756PubMedGoogle Scholar
  15. Jansson E, Esbjornsson M, Holm I, Jacobs I (1990) Increase in the proportion of fast-twitch muscle fibers by sprint training in males. Acta Physiol Scand 140:359–363PubMedCrossRefGoogle Scholar
  16. Juel C (1996) Symmetry and pH dependency of the lactate/proton carrier in skeletal muscle studied with rat sarcolemmal giant vesicles. Biochim Biophys Acta 1283:106–110PubMedCrossRefGoogle Scholar
  17. Juel C, Klarskov C, Nielsen JJ, Krustrup P, Mohr M, Bangsbo J (2004) Effect of high-intensity intermittent training on lactate and H+ release from human skeletal muscle. Am J Physiol 286:E245–E251Google Scholar
  18. Mahoney DJ, Carey K, Fu MH, Snow R, Cameron-Smith D, Parise G, Tarnapolsky MA (2004) Real-time RT-PCR analysis of housekeeping genes in human skeletal muscle following acute exercise. Physiol Genomics 18:226–231CrossRefPubMedGoogle Scholar
  19. McCullagh KJA, Poole RC, Halestrap AP, O’Brien M, Bonen A (1996) Role of the lactate transporter (MCT1) in skeletal muscles. Am J Physiol 271:E143–E150PubMedGoogle Scholar
  20. Nordsborg N, Bangsbo J, Pilegaard H (2003) Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism. J Appl Physiol 95:1201–1206PubMedGoogle Scholar
  21. Pilegaard H, Domino K, Noland T, Juel C, Hellsten Y, Halestrap AP, Bangsbo J (1999a) Effect of high-intensity exercise training on lactate/H+ transport capacity in human skeletal muscle. Am J Physiol 276:E255–E266Google Scholar
  22. Pilegaard H, Terzis G, Halestrap A, Juel C (1999b) Distribution of lactate/H+ transporter isoforms MCT1 and MCT4 in human skeletal muscle. Am J Physiol 276:E843–E848Google Scholar
  23. Pilegaard H, Ordway GA, Saltin B, Neufer PD (2000) Transcriptional regulation of gene expression in human skeletal muscle during recovery from exercise. Am J Physiol 279:E806–E814Google Scholar
  24. Schmittgen T, Zakrajsek B, Mills A, Gorn V, Singer M, Reed M (2000) Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Annal Biochem 285:194–204CrossRefGoogle Scholar
  25. Thomas C, Perrey S, Lambert K, Hugon G, Mornet D, Mercier J (2005) Monocarboxylate transporters, blood lactate removal after supramaximal exercise, and fatigue indexes in humans. J Appl Physiol 98:804–809CrossRefPubMedGoogle Scholar
  26. Wilson MC, Jackson VN, Heddle C, Price NT, Pilegaard H, Juel C, Bonen A, Montgomery I, Hutter OF, Halestrap AP (1998) Lactic acid efflux from white skeletal muscle is catalyzed by the monocarboxylate transporter isoform MCT3. J Biol Chem 273:15920–15926CrossRefPubMedGoogle Scholar
  27. Yu M, Stepto NK, Chibalin AV, Fryer LG, Carling D, Krook A, Hawley JA, Zierath JR (2003) Metabolic and mitogenic signal transduction in human skeletal muscle after intense cycling exercise. J Physiol 546(Pt 2):327–335CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Dale C. Bickham
    • 1
    • 4
  • David J. Bentley
    • 2
  • Peter F. Le Rossignol
    • 3
  • David Cameron-Smith
    • 1
  1. 1.School of Nutrition and Exercise SciencesDeakin UniversityMelbourneAustralia
  2. 2.Health and Sport Science, Department of Medical ScienceUniversity of New South WalesSydneyAustralia
  3. 3.Human Movement StudiesQueensland University of Technology BrisbaneAustralia
  4. 4.Division of Biomedical SciencesImperial College – LondonLondonUK

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