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European Journal of Applied Physiology

, Volume 93, Issue 1–2, pp 196–202 | Cite as

Effects of dietary supplementation with vitamins C and E on muscle function during and after eccentric contractions in humans

  • A. ShafatEmail author
  • P. Butler
  • R. L. Jensen
  • A. E. Donnelly
Original Article

Abstract

Reactive oxygen species may contribute to exercise-induced skeletal muscle damage, and antioxidants may protect against such damage. This study examined the effectiveness of prophylactic supplementation with vitamins C and E on symptoms of muscle damage in a single blind, two-group study design. Twelve male volunteers were randomly assigned to either treatment or control groups. The treatment group received 500 mg of vitamin C and 1,200 IU of alpha−tocopherol daily and the control group received glucose placebo for 37 days. After 30 days of treatment, volunteers performed 300 maximal eccentric contractions of the knee extensor muscles of one leg. Maximal voluntary isometric contraction force and electrically evoked force at a frequency of 20 Hz and 50 Hz were recorded before and after exercise, and on days 1, 2 and 7 after exercise. Muscle soreness questionnaires were completed and muscle girth recorded at the same time points. Eccentric contractile torque and work during the bout declined significantly in both groups (P<0.001), but this decline was smaller in the vitamin-supplemented group (P<0.05). Maximal voluntary isometric contraction force and 20:50 Hz force ratio declined significantly after exercise in both groups (P<0.01), but the decline was smaller in the treatment group on days 1 and 2 post-exercise (P<0.05). Both groups experienced similar significant muscle soreness and swelling after exercise. These data suggest that prior supplementation with dietary antioxidants ameliorates muscle functional decrements subsequent to eccentric muscle contraction.

Keywords

Antioxidants Muscle damage Reactive oxygen species Vitamin C Vitamin E 

Notes

Acknowledgements

The experiments described in the current study comply with Irish Law.

References

  1. Ashton T, Young IS, Peters JR, Jones E, Jackson SK, Davies B, Rowlands CC (1999) Electron spin resonance spectroscopy, exercise, and oxidative stress: an ascorbic acid intervention study. J Appl Physiol 87:2032–2036PubMedGoogle Scholar
  2. Bailey DM, Davies B, Young IS, Jackson MJ, Davison GW, Isaacson R, Richardson RS (2003) EPR spectroscopic detection of free radical outflow from an isolated muscle bed in exercising humans. J Appl Physiol 94:1714–1718PubMedGoogle Scholar
  3. Balnave CD, Allen DG (1995) Intracellular calcium and force in single mouse muscle fibres following repeated contractions with stretch. J Physiol (Lond) 488:25–36Google Scholar
  4. Beaton LJ, Allan DA, Tarnopolsky MA, Tiidus PM, Phillips SM (2002) Contraction-induced muscle damage is unaffected by vitamin E supplementation. Med Sci Sports Exerc 34:798–805CrossRefPubMedGoogle Scholar
  5. Brockett CL, Morgan DL, Proske U (2001) Human hamstring muscles adapt to eccentric exercise by changing optimum length. Med Sci Sports Exerc 33:783–790PubMedGoogle Scholar
  6. Brotto MA, Nosek TM (1996) Hydrogen peroxide disrupts Ca2+ release from the sarcoplasmic reticulum of rat skeletal muscle fibers. J Appl Physiol 81:731–737PubMedGoogle Scholar
  7. Brown SJ, Child RB, Day SH, Donnelly AE (1997) Exercise-induced skeletal muscle damage and adaptation following repeated bouts of eccentric muscle contractions. J Sports Sci 15:215–222CrossRefPubMedGoogle Scholar
  8. Brown SJ, Child RB, Donnelly AE, Saxton JM, Day SH (1996) Changes in human skeletal muscle contractile function following stimulated eccentric exercise. Eur J Appl Physiol Occup Physiol 72:515–521PubMedGoogle Scholar
  9. Child R, Brown S, Day S, Donnelly A, Roper H, Saxton J (1999) Changes in indices of antioxidant status, lipid peroxidation and inflammation in human skeletal muscle after eccentric muscle actions. Clin Sci (Lond) 96:105–115Google Scholar
  10. Childs A, Jacobs C, Kaminski T, Halliwell B, Leeuwenburgh C (2001) Supplementation with vitamin C and N-acetyl-cysteine increases oxidative stress in humans after an acute muscle injury induced by eccentric exercise. Free Radic Biol Med 31:745–753CrossRefPubMedGoogle Scholar
  11. Clarkson PM, Tremblay I (1988) Exercise-induced muscle damage, repair and adaptation in humans. J Appl Physiol 65:1–6PubMedGoogle Scholar
  12. Edwards RH, Hill DK, Jones DA, Merton PA (1977) Fatigue of long duration in human skeletal muscle after exercise. J Physiol (Lond) 272:769–778Google Scholar
  13. Ingalls CP, Warren GL, Williams JH, Ward CW, Armstrong RB (1998) E-C coupling failure in mouse EDL muscle after in vivo eccentric contractions. J Appl Physiol 85:58–67PubMedGoogle Scholar
  14. Ji LL (1999) Antioxidants and oxidative stress in exercise. Proc Soc Exp Biol Med 222:283–292CrossRefPubMedGoogle Scholar
  15. Jones DA, Newham DJ, Round JM, Tolfree SE (1986) Experimental human muscle damage: morphological changes in relation to other indices of damage. J Physiol (Lond) 375:435–448Google Scholar
  16. Kaminski M, Boal R (1992) An effect of ascorbic acid on delayed-onset muscle soreness. Pain 50:317–321CrossRefPubMedGoogle Scholar
  17. Kourie JI (1998) Interaction of reactive oxygen species with ion transport mechanisms. Am J Physiol 275: C1–24PubMedGoogle Scholar
  18. Lynn R, Morgan DL (1994) Decline running produces more sarcomeres in rat vastus intermedius muscle fibers than does incline running. J Appl Physiol 77:1439–1444PubMedGoogle Scholar
  19. Mackey AL, Donnelly AE, Turpeenniemi-Hujanen T, Roper HP (2004) Skeletal muscle collagen content in humans following high force eccentric contractions. J Appl Physiol 97:197–203CrossRefPubMedGoogle Scholar
  20. Maxwell SR, Jakeman P, Thomason H, Leguen C, Thorpe GH (1993) Changes in plasma antioxidant status during eccentric exercise and the effect of vitamin supplementation. Free Radic Res Commun 19:191–202PubMedGoogle Scholar
  21. McBride JM, Kraemer WJ, Triplett-McBride T, Sebastianelli W (1998) Effect of resistance exercise on free radical production. Med Sci Sports Exerc 30:67–72PubMedGoogle Scholar
  22. Morgan DL (1990) New insights into the behavior of muscle during active lengthening. Biophys J 57:209–221PubMedGoogle Scholar
  23. Morgan DL, Allen DG (1999) Early events in stretch-induced muscle damage. J Appl Physiol 87:2007–2015PubMedGoogle Scholar
  24. Petersen EW, Ostrowski K, Ibfelt T, Richelle M, Offord E, Halkjaer-Kristensen J, Pederson BT (2001) Effect of vitamin supplementation on cytokine response and on muscle damage after strenuous exercise. Am J Physiol 280:C1570-C1575Google Scholar
  25. Posterino GS, Lamb GD (1996) Effects of reducing agents and oxidants on excitation-contraction coupling in skeletal muscle fibres of rat and toad. J Physiol (Lond) 496:809–825Google Scholar
  26. Saxton JM, Donnelly AE, Roper HP (1994) Indices of free-radical-mediated damage following maximum voluntary eccentric and concentric muscular work. Eur J Appl Physiol Occup Physiol 68:189–193PubMedGoogle Scholar
  27. Thompson D, Williams C, Kingsley M, Nicholas CW, Lakomy HKA, McArdle F, Jackson MJ (2001) Muscle soreness and damage parameters after prolonged intermittent shuttle-running following acute vitamin C supplementation. Int J Sports Med 22:68–75CrossRefPubMedGoogle Scholar
  28. Thompson D, Williams C, Garcia-Roves P, McGregor SJ, McArdle F, Jackson MJ (2003) Post-exercise vitamin C supplementation and recovery from demanding exercise. Eur J Appl Physiol 89:393–400PubMedGoogle Scholar
  29. Van Der Meulen JH, McArdle A, Jackson MJ, Faulkner JA (1997) Contraction-induced injury to the extensor digitorum longus muscles of rats: the role of vitamin E. J Appl Physiol 83:817–823PubMedGoogle Scholar
  30. Warren GL, Ingalls CP, Lowe DA, Armstrong RB (2001) Excitation-contraction uncoupling: major role in contraction-induced muscle injury. Exerc Sport Sci Rev 29:82–87CrossRefPubMedGoogle Scholar
  31. Warren GL, Lowe DA, Hayes DA, Karwoski CJ, Prior BM, Armstrong RB (1993) Excitation failure in eccentric contraction-induced injury of mouse soleus muscle. J Physiol 468:487–499PubMedGoogle Scholar
  32. Yu BP (1994) Cellular defenses against damage from reactive oxygen species. Physiol Rev 74:139-162PubMedGoogle Scholar
  33. Zerba E, Komorowski TE, Faulkner JA (1990) Free radical injury to skeletal muscles of young, adult, and old mice. Am J Physiol 258:C429–C435PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • A. Shafat
    • 1
    Email author
  • P. Butler
    • 1
  • R. L. Jensen
    • 1
    • 2
  • A. E. Donnelly
    • 1
  1. 1.Department of Physical Education and Sport SciencesUniversity of LimerickLimerickIreland
  2. 2.Department of Health Physical Education & RecreationNorthern Michigan UniversityMarquetteUSA

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