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European Journal of Applied Physiology

, Volume 92, Issue 4–5, pp 540–547 | Cite as

Muscle buffer capacity and aerobic fitness are associated with repeated-sprint ability in women

  • David Bishop
  • Johann Edge
  • Carmel Goodman
Original Article

Abstract

In addition to a high aerobic fitness, the ability to buffer hydrogen ions (H+) may also be important for repeated-sprint ability (RSA). We therefore investigated the relationship between muscle buffer capacity (βmin vivo and βmin vitro) and RSA. Thirty-four untrained females [mean (SD): age 19 (1) years, maximum oxygen uptake (O2peak) 42.3 (7.1) ml·kg−1·min−1] completed a graded exercise test (GXT), followed by a RSA cycle test (five 6-s sprints, every 30 s). Capillary blood was sampled during the GXT and before and after the RSA test to determine blood pH (pHb) and lactate concentration ([La]b). Muscle biopsies were taken before (n=34) and after (n=23) the RSA test to determine muscle lactate concentration ([La]i), hydrogen ion concentration ([H+]i) pHi, βmin vivo and βmin vitro. There were significant correlations between work decrement (%) and βmin vivo (r=−0.72, P<0.05), O2peak (r=−0.62, P<0.05), lactate threshold (LT) (r=−0.56, P<0.05) and changes in [H+]i (r=0.41, P<0.05). There were however, no significant correlations between work decrement and βmin vitro, or changes in [La]i, or [La]b. There were also no significant correlations between total work (J·kg−1) during the RSA test and βmin vitro, βmin vivo, or changes in [La]i, pHi, [La]b, or pHb. There were significant correlations between total work (J·kg−1) and bothO2peak (r=0.60, P<0.05) and LT(r=0.54, P<0.05). These results support previous research, identifying a relationship between RSA and aerobic fitness. This study is the first to identify a relationship between βmin vivo and RSA. This suggests that the ability to buffer H+ may be important for maintaining performance during brief, repeated sprints.

Keywords

Hydrogen ions Intermittent exercise Lactate threshold Team sports 

References

  1. Bassett DR. Howley ET (2000) Limiting factors for maximum oxygen uptake and determinants of endurance performance. Med Sci Sports Exerc 32:70–84PubMedGoogle Scholar
  2. Bishop D, Jenkins DG, Mackinnon LT (1998) The relationship between plasma lactate parameters, W peak and 1-h cycling performance in women. Med Sci Sports Exerc 30:1270–1275PubMedGoogle Scholar
  3. Bishop D, Spencer M, Duffield R, Lawrence S (2001) The validity of a repeated sprint ability test. J Sci Med Sport 4:19–29PubMedGoogle Scholar
  4. Bishop D, Lawrence S, Spencer M (2003) Predictors of repeated-sprint ability in elite female hockey players. J Sci Med Sport 6:199–209PubMedGoogle Scholar
  5. Bishop D, Davis C, Edge J, Goodman C (2004) Induced metabolic alkalosis affects muscle metabolism and repeated-sprint ability. Med Sci Sports Exerc 36:807–813PubMedGoogle Scholar
  6. Bogdanis GC, Nevill ME, Boobis LH, Lakomy HKA (1996) Contribution of phosphocreatine and aerobic metabolism to energy supply during repeated sprint exercise. J Appl Physiol 80:876–884Google Scholar
  7. Chin ER, Allen DG (1998) The contribution of pH-dependent mechanisms to fatigue at different intensities in mammalian single muscle fibres. J Physiol (Lond) 512:831–840Google Scholar
  8. Coyle EF, Feltner ME, Kautz SA, et al (1991) Physiological and biomechanical factors associated with elite endurance cycling performance. Med Sci Sports Exerc 23:93–107PubMedGoogle Scholar
  9. Dawson B, FitzSimons M, Ward D (1993) The relationship of repeated sprint ability to aerobic power and performance measures of anaerobic work capacity and power. Aust J Sci Med Sport 25:88–93Google Scholar
  10. Edge J, Bishop D, Goodman C, Dawson B (2002) The effects of training intensity on muscle buffer capacity and repeated-sprint ability. European Congress of Sport Science, Athens, Greece, p 622Google Scholar
  11. Fabiato A, Fabiato F (1978) Effects of pH on the myofilaments and the sarcoplasmic reticulum of skinned cells from the cardiac and skeletal muscles. J Physiol (Lond) 276:233–255Google Scholar
  12. FitzSimons M, Dawson B, Ward D, Wilkinson A (1993) Cycling and running tests of repeated sprint ability. Aust J Sci Med Sport 25:82–87Google Scholar
  13. Fornasiero D, Martin DT, Brosnan MJ, et al (1999) Effects of altitude training on repeat sprint and graded exercise test performance in female road cyclists. Fifth IOC World Congress on Sport Sciences, p 90Google Scholar
  14. Gaitanos GC, Williams C, Boobis LH, Brooks S (1993) Human muscle metabolism during intermittent maximal exercise. J Appl Physiol 75:712–719Google Scholar
  15. Gore CJ, Hahn AG, Aughey RJ, et al (2001) Live high:train low increases muscle buffer capacity and submaximal cycling efficiency. Acta Physiol Scand 173:275–286PubMedGoogle Scholar
  16. Harris C, Edwards RHT, Hultman E, Nordesjo LO, Nylind B (1976) The time course of phosphorylcreatine resynthesis during recovery of the quadriceps muscle in man. Pflugers Arch 367:137–142PubMedGoogle Scholar
  17. Harris RC, Hultman E, Nordesjo LO (1974) Glycogen, glycolytic intermediates and high-energy phosphates determined in biopsy samples of musculus quadriceps femoris of man at rest: methods and variance of values. Scand J Clin Lab Invest 33:109–120PubMedGoogle Scholar
  18. Hermansen L (1981) Muscle fatigue during maximal exercise of short duration. In: Prampero PE di, Poortmans J (eds) Physiological chemistry of exercise and training. medicine and sport science. Karger, Basel, pp 45–52Google Scholar
  19. Holloszy JO, Coyle EF (1984) Adaptations of skeletal muscle to endurance exercise and their metabolic consequences. J Appl Physiol 56:831–838PubMedGoogle Scholar
  20. Hultman E, Sahlin K (1980) Acid-base balance during exercise. Exerc Sport Sci Rev 8:41–128PubMedGoogle Scholar
  21. Ivy JL, Costill DL, Maxwell BD (1980) Skeletal muscle determinants of maximum aerobic power in man. Eur J Appl Physiol 44:1–8Google Scholar
  22. Juel C (1998) Skeletal muscle Na+/H+ exchange in rats: pH dependency and the effect of training. Acta Physiol Scand 164:135–140CrossRefPubMedGoogle Scholar
  23. Kowalchuk JM, Heigenhauser GJF, Lindinger MI, Obminski G, Sutton JR, Jones NL (1988) Role of lungs and inactive muscle in acid-base control after maximal exercise. J Appl Physiol 65:2090–2096PubMedGoogle Scholar
  24. Krustup P, Mohr M, Amstrup T, et al (2003) The yo-yo intermittent recovery test: physiological response, reliability and validity. Med Sci Sports Exerc 35:697–705PubMedGoogle Scholar
  25. Larson-Meyer DE, Newcomer BR, Hunter GR, Joanisse DR, Weinsier RL, Bamman MM (2001) Relation between in vivo and in vitro measurements of skeletal muscle metabolism. Muscle Nerve 24:1665–1676CrossRefPubMedGoogle Scholar
  26. Mannion AF, Jakeman PM, Willan PLT (1993) Determination of human skeletal muscle buffer value by homogenate technique: methods of measurement. J Appl Physiol 75:1412–1418Google Scholar
  27. McCully KK, Kakihira H, Vandenborne K, Kent-Braun J (1991) Noninvasive measurements of activity-induced changes in muscle metabolism. J Biomech 21 [Suppl 1]:153–161Google Scholar
  28. McCully KK, Fielding RA, Evans WJ, Leigh JS, Posner JD (1993) Relationships between in vivo and in vitro measurements of metabolism in young and old human calf muscles. J Appl Physiol 75:813–819Google Scholar
  29. McMahon S, Wenger HA (1998) The relationship between aerobic fitness and both power output and subsequent recovery during maximal intermittent exercise. J Sci Med Sport 1:219–227PubMedGoogle Scholar
  30. Nevill ME, Boobis LH, Brooks S, Williams C (1989) Effect of training on muscle metabolism during treadmill sprinting. J Appl Physiol 67:2376–2382Google Scholar
  31. Sahlin K, Henriksson J (1984) Buffer capacity and lactate accumulation in skeletal muscle of trained and untrained men. Acta Physiol Scand 122:331–339PubMedGoogle Scholar
  32. Sjödin B, Jacobs I, Svedenhag J (1982) Changes in onset of blood lactate accumulation and muscle enzymes after training at OBLA. Eur J Appl Physiol 49:45–57Google Scholar
  33. Takahashi H, Inaki M, Fujimoto K, Katsuta S, Anno I, Niitsu M, Itai Y (1995) Control of the rate of phosphocreatine resynthesis after exercise in trained and untrained human quadriceps muscles. Eur J Appl Physiol 71:396–404Google Scholar
  34. Walter G, Vandenborne K, McCully KK, Leigh JS (1997) Noninvasive measurement of phosphocreatine recovery kinetics in single human muscles. Am J Physiol 272:C525–C535PubMedGoogle Scholar
  35. Westerblad H, Bruton J, Lannergren J (1997) The effect of intracellular pH on contractile function of intact, single fibres of mouse declines with increasing temperature. J Physiol (Lond) 500:193–204Google Scholar
  36. Weston AR, Wilson GR, Noakes TD, Myburgh KH (1996) Skeletal muscle buffering capacity is higher in the superficial vastus than in the sloeus of spontaneously running rats. Acta Physiol Scand 157:211–216CrossRefPubMedGoogle Scholar
  37. Weston AR, Myburgh KH, Lindsay FH, Dennis SC, Noakes TD, Hawley JA (1997) Skeletal muscle buffering capacity and endurance performance after high-intensity interval training by well-trained cyclists. Eur J Appl Physiol 75:7–13Google Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Team Sport Research Group, School of Human Movement and Exercise ScienceThe University of Western AustraliaCrawleyAustralia

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