European Journal of Applied Physiology

, Volume 91, Issue 5–6, pp 737–740 | Cite as

Magnetic resonance imaging evaluation of cooling on blood flow and oedema in skeletal muscles after exercise

  • Osamu Yanagisawa
  • Hiroki Kudo
  • Nobuyuki Takahashi
  • Hiroshi Yoshioka
Original Article


This study assessed the effects of cooling on blood flow and intramuscular water content in human skeletal muscles after exercise using magnetic resonance imaging. In six male subjects, their legs were randomly assigned to be control or to be cooled. All subjects performed ankle dorsiflexion exercise inside an imaging magnet and after exercise, an ice bag was placed on the ankle dorsiflexors of the cooled leg. Flow-sensitive images, which reflect both perfusion and intramuscular water, were obtained before and up to 270 s post-exercise at 30-s intervals. The flow-sensitive alternating inversion recovery (FAIR) images, which extract only the perfusion change, were also obtained. Signal intensity (SI) in the ankle dorsiflexors was estimated before and after exercise in both flow-sensitive and FAIR images. On the flow-sensitive images, the control leg increased SI 30–270 s after exercise (P<0.05), but the cooled leg showed no significant change. On the FAIR images, the control leg increased SI 30–270 s post-exercise (P<0.05), while the cooled leg increased SI 30–150 s and 210 s after exercise (P<0.05). The findings suggest that cooling attenuates the perfusion elevation and prevents the oedema formation in skeletal muscle immediately after exercise.


Flow-sensitive image Flow-sensitive alternating inversion recovery image Perfusion Intramuscular water Cryotherapy 


  1. Clarke RSJ, Hellon RF, Lind AR (1958) Vascular reactions of the human forearm to cold. Clin Sci 17:165–179Google Scholar
  2. Coté DJ, Prentice WE, Hooker DN, Shields EW (1988) Comparison of three treatment procedures for minimizing ankle sprain swelling. Phys Ther 68:1072–1076PubMedGoogle Scholar
  3. Daanen HAM, Van de Linde FJG, Romet TT, Ducharme MB (1997) The effect of body temperature on the hunting response of the middle finger skin temperature. Eur J Appl Physiol 76:538–543Google Scholar
  4. Dolan MG, Thornton RM, Fish DR, Mendel FC (1997) Effects of cold water immersion on edema formation after blunt injury to the hind limbs of rats. J Athl Train 32:233–237Google Scholar
  5. Fleckenstein JL, Canby RC, Parkey RW, Peshock RM (1988) Acute effects of exercise on MR imaging of skeletal muscle in normal volunteers. Am J Roentgenol 151:231–237Google Scholar
  6. Fleckenstein JL, Haller RG, Lewis SF, Archer BT, Barker BR, Payne J, Parkey RW, Peshock RM (1991) Absence of exercise-induced MRI enhancement of skeletal muscle in McArdle’s disease. J Appl Physiol 71:961–969Google Scholar
  7. Fox RH, Wyatt HT (1962) Cold-induced vasodilatation in various areas of the body surface of man. J Physiol (Lond) 162:289–297Google Scholar
  8. Ho SSW, Illgen RL, Meyer RW, Torok PJ, Cooper MD, Reider B (1995) Comparison of various icing times in decreasing bone metabolism and blood flow in the knee. Am J Sports Med 23:74–76PubMedGoogle Scholar
  9. Karunakara RG, Lephart SM, Pincivero DM (1999) Changes in forearm blood flow during single and intermittent cold application. J Orthop Sports Phys Ther 29:177–180PubMedGoogle Scholar
  10. Le Rumeur E, De Certaines J, Toulouse P, Rochcongar P (1987) Water phases in rat striated muscles as determined by T2 proton NMR relaxation times. Magn Reson Imaging 5:267–272PubMedGoogle Scholar
  11. Lewis T (1930) Observations upon reactions of vessels in human skin to cold. Heart 15:177–208Google Scholar
  12. Matsen FA, Questad K, Matsen AL (1975) The effects of local cooling on postfracture swelling: A controlled study. Clin Orthop 109:201–206PubMedGoogle Scholar
  13. Maw GJ, Mackenzie IL, Taylor NAS (1998) Human body-fluid distribution during exercise in hot, temperate, and cool environments. Acta Physiol Scand 163:297–304CrossRefPubMedGoogle Scholar
  14. McMaster WC, Liddle S (1980) Cryotherapy influence on posttraumatic limb edema. Clin Orthop 150:283–287PubMedGoogle Scholar
  15. Paddon-Jones DJ, Quigley BM (1997) Effect of cryotherapy on muscle soreness and strength following eccentric exercise. Int J Sports Med 18:588–593PubMedGoogle Scholar
  16. Sendowski I, Savourey G, Besnard Y, Bittel J (1997) Cold induced vasodilatation and cardiovascular responses in humans during cold water immersion of various upper limb areas. Eur J Appl Physiol 75:471–477Google Scholar
  17. Sendowski I, Savourey G, Launay J-C, Besnard Y, Cottet-Emard J-M, Pequignot J-M, Bittel J (2000) Sympathetic stimulation induced by hand cooling alters cold-induced vasodilatation in humans. Eur J Appl Physiol 81:303–309Google Scholar
  18. Shepherd JT, Rusch NJ, Vanhoutte PM (1983) Effect of cold on the blood vessel wall. Gen Pharmacol 14:61–64CrossRefPubMedGoogle Scholar
  19. Sjøgaard G, Saltin B (1982) Extra- and intracellular water spaces in muscles of man at rest and with dynamic exercise. Am J Physiol 243:R271–R280PubMedGoogle Scholar
  20. Swenson C, Swärd L, Karlsson J (1996) Cryotherapy in sports medicine. Scand J Med Sci Sports 6:193–200PubMedGoogle Scholar
  21. Taber C, Contryman K, Fahrenbruch J, LaCount K, Cornwall M (1992) Measurement of reactive vasodilatation during cold gel pack application to nontraumatized ankles. Phys Ther 72:294–299PubMedGoogle Scholar
  22. Thorsson O, Lilja B, Ahlgren L, Hemdal B, Westlin N (1985) The effect of local cold application on intramuscular blood flow at rest and after running. Med Sci Sports Exerc 17:710–713PubMedGoogle Scholar
  23. Yanagisawa O, Niitsu M, Yoshioka H, Goto K, Kudo H, Itai Y (2003) The use of magnetic resonance imaging to evaluate the effects of cooling on skeletal muscle after strenuous exercise. Eur J Appl Physiol 89:53–62PubMedGoogle Scholar
  24. Yoshioka H, Itai Y (1996) Magnetic resonance imaging of isolated skeletal muscles: Osmotic influence. Invest Radiol 31:359–363CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Osamu Yanagisawa
    • 1
  • Hiroki Kudo
    • 2
  • Nobuyuki Takahashi
    • 2
  • Hiroshi Yoshioka
    • 3
  1. 1.Japan Institute of Sports SciencesTokyoJapan
  2. 2.Doctoral Program in Graduate School of Comprehensive Human SciencesUniversity of TsukubaIbarakiJapan
  3. 3.Department of Radiology, Institute of Clinical MedicineUniversity of TsukubaIbarakiJapan

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