Exposure to field vs. storage wheat dust: different consequences on respiratory symptoms and immune response among grain workers
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The aim of this study was to understand the differential acute effects of two distinct wheat-related dusts, such as field or stored wheat dust handling, on workers’ health and how those effects evolved at 6 month intervals.
Exposure, work-related symptoms, changes in lung function, and blood samples of 81 workers handling wheat and 61 controls were collected during the high exposure season and 6 months after. Specific IgG, IgE, and precipitins against 12 fungi isolated from wheat dust were titrated by enzyme-linked immunosorbent assay, dissociation-enhanced lanthanide fluorescence immunoassay, and electrosyneresis. The level of fungi was determined in the workers’ environment. Levels of exhaled fraction of nitrogen monoxide (FENO) and total IgE were obtained. Exposure response associations were investigated by mixed logistic and linear regression models.
The recent exposure to field wheat dust was associated with a higher prevalence for five of six self-reported airway symptoms and with a lower FENO than those in the control population. Exposure to stored wheat dust was only associated with cough. No acute impact of exposure on respiratory function was observed. Exposure to field wheat dust led to workers’ sensitization against the three field fungi Aureobasidum, Cryptococcus, and Phoma, although exposure to storage wheat dust was associated with tolerance. The level of Ig remained stable 6 months after exposure.
The clinical picture of workers exposed to field or storage wheat dust differed. The systematic characterization of the aerosol microbial profile may help to understand the reasons for those differences.
KeywordsGrain workers Cattle raisers Respiratory symptoms Occupational wheat dust exposure Fungi-specific immunoglobulins
We thank Dr. C. Lazor-Blanchet and M. Coté for their support recruiting the controls, M. Porchet and D. Spinedi for helping with data collection, Y. Randin for data management supportand BioMed Proofreading® LLC experts to correct the English.
The study was funded by the ANSES (Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail, France) Grant 2011/1/087 to HNH and by a SUVA (Caisse nationale suisse d’assurance en cas d’accidents) grant to PK and BD. The funding organs played no role in the collection of data, formulation of study hypothesis analyses, or interpretation of findings.
Compliance with ethical standards
Conflict of interest
The authors declare no conflicts of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
- Barrera C, Richaud-Thiriez B, Rocchi S, Rognon B, Roussel S, Grenouillet F, Laboissiere A, Dalphin JC, Reboux G, Millon L (2016) New commercially available IgG kits and time-resolved fluorometric IgE assay for allergic broncho-pulmonary aspergillosis diagnosis in cystic fibrosis patients. Clin Vaccine Immunol 23:196–203CrossRefGoogle Scholar
- Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336CrossRefGoogle Scholar
- Dalphin J-C, Reboux G, Lefebvre A, Thaon I (2009) Pneumopahties d’hypersensibilité. In: Traité de Pneumologie (ed) Flammarion, ParisGoogle Scholar
- Gora A, Mackiewicz B, Krawczyk P, Golec M, Skorska C, Sitkowska J, Cholewa G, Larsson L, Jarosz M, Wojcik-Fatla A, Dutkiewicz J (2009) Occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in Poland. Ann Agric Environ Med 16:143 – 50Google Scholar
- Halstensen AS, Heldal KK, Wouters IM, Skogstad M, Ellingsen DG, Eduard W (2013) Exposure to grain dust and microbial components in the Norwegian grain and compound feed industry. Ann Occup Hyg 57:1105–1114Google Scholar
- Jouneau S, Boche A, Brinchault G, Fekete K, Guillot S, Bayat S, Desrues B (2012) On-site screening of farming-induced chronic obstructive pulmonary disease with the use of an electronic mini-spirometer: results of a pilot study in Brittany, France. Int Arch Occup Environ Health 85:623–630CrossRefGoogle Scholar
- Le Bars J, Escoula L, Henry G (1973) Mycoflore des fourrages secs, Inventaire et fréquence des espèces. Ann Rech Vet 4:273–282Google Scholar
- Lucas D, Lodde B, Pougnet RP, Dewitte JD, Jegaden D (2013) Evaluation of the sensitisation to grains and its pulmonary impact in employees of the port of Brest silos. Int Marit Health 64:18–23Google Scholar
- Minette A (1989) Questionnaire of the European Community for Coal and Steel (ECSC) on respiratory symptoms. 1987—updating of the 1962 and 1967 questionnaires for studying chronic bronchitis and emphysema. Eur Respir J 2:165–177Google Scholar
- Roussel S, Rognon B, Barrera C, Reboux G, Salamin K, Grenouillet F, Thaon I, Dalphin J-C, Tillie-Leblond I, Quadroni M, Monod M, Millon L (2011a) Immuno-reactive proteins from Mycobacterium immunogenum useful for serodiagnosis of metalworking fluid hypersensitivity pneumonitis. Int J Med Microbiol 301:150–156CrossRefGoogle Scholar
- Spankie S, Cherrie JW (2012) Exposure to grain dust in Great Britain. Ann Occup Hyg 56:25–36Google Scholar
- Swan JR, Crook B (1998) Airborne microorganisms associated with grain handling. Ann Agric Environ Med 5:7–15Google Scholar