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Biomarkers of inflammation in workers exposed to compost and sewage dust

  • Kari Kulvik HeldalEmail author
  • Lars Barregard
  • Dag G. Ellingsen
Original Article

Abstract

Purpose

The association between exposure during handling of sewage and compost and the serum concentration of inflammatory biomarkers was studied.

Methods

A total of 44 workers exposed to sewage dust, 47 workers exposed to compost dust and 38 referents from the administrative staff participated. Microbial aerosols were collected by personal inhalable samplers. The concentrations of bacterial cells, spores from fungi and bacteria (actinomycetes) and endotoxins were determined by fluorescence and scanning electron microscopy and the Limulus assay. Fibrinogen, D-dimer, ICAM-1, VCAM-1 and IL-6 were determined by ELISA and C-reactive protein (CRP) by HS-MicroCRP assay in blood samples collected post-shift.

Results

The exposure to dust ranged from 0.02 to 11 mg/m3, endotoxins from 1 to 3160 EU/m3 and bacteria from 0 to 209 × 106 cells/m3. Fungal (0–41 × 106 spores/m3) and actinomycetes spores (0–590 × 106 actinomycetes spores/m3) were observed only at compost plants. The exposed workers had significantly higher fibrinogen (arithmetic mean 3.3 mg/ml) and CRP (geometric mean 1.5 mg/L) compared to the referents (2.8 and 1.0 mg/L, respectively). The serum concentration of CRP was negatively associated with forced expiratory volume in one second (FEV1) in  % of predicted. Exposure to inhalable dust and bacteria was positively associated with the serum concentration of ICAM-1.

Conclusions

This study suggests that exposure to bacteria and dust when handling sewage and compost may initiate an inflammation shown by an increase in serum concentration of ICAM-1. The higher concentrations of fibrinogen and CRP in exposed workers compared to the referents may reflect a low-grade systemic inflammation.

Keywords

Exposure Bacteria Endotoxins Actinomycetes ICAM CRP 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Kari Kulvik Heldal
    • 1
    Email author
  • Lars Barregard
    • 2
  • Dag G. Ellingsen
    • 1
  1. 1.National Institute of Occupational HealthOsloNorway
  2. 2.Department of Occupational and Environmental MedicineUniversity of GothenburgGothenburgSweden

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