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Increase of urinary concentrations of 8-hydroxy-2′-deoxyguanosine in diesel exhaust emission inspector exposed to polycyclic aromatic hydrocarbons

  • Mei-Wen Lee
  • Mei-Lien Chen
  • Shih-Chun Candice Lung
  • Chung-Jung Tsai
  • Chao-Feng Steven Lai
  • Shang-Chun Yang
  • I-Fang MaoEmail author
Original Article

Abstract

Purpose

The objectives of this study were to explore the factors influencing urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels in diesel engine exhaust emission inspectors (inspectors), the association between polycyclic aromatic hydrocarbons (PAHs) exposure and fine particulate matter (PM2.5) levels in diesel exhaust particles (DEPs), and the PAHs exposure levels in diesel vehicle emission inspection stations (inspection stations).

Methods

Twenty-eight inspectors and a control group of thirty-eight individuals matched by age and gender were recruited for this study. Fifteen ambient air samples and eighty-four personal air samples were monitored during 3-day work periods using a repeated-measures study design in each inspection station. Airborne samples were analyzed with a fluorescence detector and by high-performance liquid chromatography. Urinary 8-OHdG was measured in 168 pre- and post-work urine samples from inspectors, and in 38 urine samples from controls.

Results

The concentrations of PAHs in DEP2.5 (PM2.5 in DEPs) were significantly and positively related to urinary log10 8-OHdG levels after adjusting for smoking status and BMI. Statistically, there was a significant correlation between air log10 PAHs and air log10 PM2.5 concentrations in inspectors. Fifteen PAHs compounds within DEP2.5 revealed the concentrations ranged from 5.18 to 22.93 ng/m3 in ambient air monitoring and 1.03 to 12.60 ng/m3 in personal air monitoring.

Conclusions

This study is the first to indicate an association between occupational PAHs exposure from DEP2.5 at an inspection station and an increased excretion of urinary 8-OHdG in inspectors. In addition, this study also found smoking is not a confounder in inspectors exposed to PAHs in DEP2.5.

Keywords

Diesel exhaust Polycyclic aromatic hydrocarbons Urinary 8-hydroxy-2′-deoxyguanosine Occupational exposure 

Notes

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Mei-Wen Lee
    • 1
    • 2
  • Mei-Lien Chen
    • 1
  • Shih-Chun Candice Lung
    • 3
  • Chung-Jung Tsai
    • 4
  • Chao-Feng Steven Lai
    • 5
  • Shang-Chun Yang
    • 1
  • I-Fang Mao
    • 6
    • 7
    Email author
  1. 1.Institute of Environmental and Occupational Health SciencesNational Yang-Ming UniversityTaipeiTaiwan
  2. 2.Department of Healthcare AdministrationCentral Taiwan University of Science and TechnologyTaichungTaiwan
  3. 3.Research Center for Environmental ChangesAcademia SinicaTaipeiTaiwan
  4. 4.Department of Occupational Safety and HealthChung Hwa University of Medical TechnologyRende Shiang, Tainan CountyTaiwan
  5. 5.Department of Landscape ArchitectureMingDao UniversityChanghuaTaiwan
  6. 6.Department of Occupational Safety and HealthChung Shan Medical UniversityTaichungTaiwan
  7. 7.Department of Medical ResearchChung Shan Medical University HospitalTaichungTaiwan

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