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Polybrominated diphenyl ethers—plasma levels and thyroid status of workers at an electronic recycling facility

  • A. Julander
  • M. Karlsson
  • K. Hagström
  • C. G. Ohlson
  • M. Engwall
  • I-L. Bryngelsson
  • H. Westberg
  • B. van Bavel
Original Article

Abstract

Objectives: Personnel working with electronic dismantling are exposed to polybrominated diphenyl ethers (PBDEs), which in animal studies have been shown to alter thyroid homeostasis. The aim of this longitudinal study was to measure plasma level of PBDEs in workers at an electronic recycling facility and to relate these to the workers’ thyroid status. Methods: PBDEs and three thyroid hormones: triiodothyronine (T3), thyroxin (T4) and thyroid stimulating hormone (TSH) were repeatedly analysed in plasma from 11 workers during a period of 1.5 years. Results: Plasma levels of PBDEs at start of employment were <0.5–9.1 pmol/g lipid weight (l.w.). The most common congener was PBDE #47 (median 2.8 pmol/g l.w.), followed by PBDE #153 (median 1.7 pmol/g l.w.), and PBDE #183 had a median value of <0.19 pmol/g l.w. After dismantling the corresponding median concentrations were: 3.7, 1.7 and 1.2 pmol/g l.w., respectively. These differences in PBDE levels were not statistically significant. PBDE #28 showed a statistically significantly higher concentration after dismantling than at start of employment (P=0.016), although at low concentrations (start 0.11 pmol/g l.w. and dismantling 0.26 pmol/g l.w.). All measured levels of thyroid hormones (T3, T4 and TSH) were within the normal physiological range. Statistically significant positive correlations were found between T3 and #183 in a worker, between T4 and both #28 and #100 in another worker and also between TSH and #99 and #154 in two workers. Conclusions: The workers’ plasma levels of PBDEs fluctuated during the study period. Due to small changes in thyroid hormone levels it was concluded that no relevant changes were present in relation to PBDE exposure within the workers participating in this study.

Keywords

Diphenyl ethers Thyroid status Recycling factory 

Notes

Acknowledgements

Mrs Ann-Marie Porat at Haluxa for help with the blood sampling, Ms Britt-Marie Isaksson for preparing the plasma samples and Wellington Laboratories for supplying several PBDE standards. We are thankful to Mrs Cecilia Fedeli, for help with the statistical analysis and interpretation of the result. The Knowledge Foundation is acknowledged for financial support. Last but not least, we would like to express our gratitude to the management and the workers at Wåab Electronics for participating in the study.

References

  1. Andersson Ö, Blomkvist G (1981) Polybrominated aromatic pollutants in fish in Sweden. Chemosphere 10:1051–1060CrossRefGoogle Scholar
  2. Asplund L, Malmvärn A, Marsh G, Athanasiadou M, Bergman Å, Kautsky L (2001) Hydroxylated brominated diphenyl ethers in salmon (Salmo salar), blue mussel (Mytilus edulis) and the red algae (Ceramium tenuicorne) from the Baltic Sea—natural production in the Baltic Sea biota. Organohal Comp 52:67–70Google Scholar
  3. Athanasiadou M, Marsh G, Athanasiadous I, Asplund L, Bergman Å (2003) Chromatographic and mass spectrometric characteristics of polybrominated diphenyl ethers (MeO-PBDEs). Organohal Comp 61:25–28Google Scholar
  4. Bahn A, Mills J, Snyder P, Gann P, Houten L, Bialik O, Hollman L, Utiger R (1980) Hypothyroidism in workers exposed to polybrominated biphenyls. N Engl J Med 302:31–33PubMedGoogle Scholar
  5. van Bavel B, Hardell L, Kitti A, Liljedahl M, Karlsson M, Pettersson A, Tysklind M, Lindström G (2002) High levels of PBDEs in 5% of 220 blood samples from the Swedish population. Organohal Comp 58:161–164Google Scholar
  6. de Boer J, Wester PG, Rodriguez DP, Lewis W, Boon JP (1998) Polybrominated biphenyls and diphenyls in sperm whale and other marine mammals—a new threat to ocean life? Organohal Comp 35:383–386Google Scholar
  7. Brown H, Prescott R (1999) Applied mixed models in medicine. In: Barnett V (ed) Statistics in practice. Wiley, ChichesterGoogle Scholar
  8. BSEF (2003) Major brominated flame retardants volume estimates, BSEF. 2003. http://www.bsef-site.com/docs/BFR_vol_2001.doc
  9. Darnerud P, Eriksen G, Jóhannesson T, Larsen P, Viluksela M (2001) Polybrominated diphenyl ethers: occurrence, dietary exposure, and toxicology. Environ Health Perspect 10(suppl):49–68Google Scholar
  10. Domingo JL (2004) Human exposure to polybrominated diphenyl ethers through the diet. J Chromatogr A 1054:321–326CrossRefPubMedGoogle Scholar
  11. Fängström B (2004) A retrospective study of PBDEs in human milk; and PBDE, PCDD/F, PCB, and levels in US human blood (1973 and 2003). BFR 2004. In: Proceedings of the third international workshop on brominated flame retardants, pp 33–36Google Scholar
  12. Fowles JR, Fairbrother A, Baecher-Steppan L, Kerkvliet N (1994) Immunologic and endocrine effects of the flameretardant pentabromodiphenyl ether (DE-71) in C57BL/6J mice. Toxicology 86:49–61CrossRefPubMedGoogle Scholar
  13. Grimvall E, Rylander L, Nilsson-Ehle P, N U, Strömberg U, Hagmar L, Östman C (1997) Monitoring of polychlorinated biphenyls in human blood plasma: methodological developments and influence of age, lactation and fish consumption. Arch Environ Contam Toxicol 32:329–336CrossRefPubMedGoogle Scholar
  14. Haglund P, Zook D, Buser H-R, Hu J (1997) Identification and quantification of polybrominated diphenyl ethers and methoxy-polybrominated diphenyl ethers in Baltic biota. Environ Sci Techn 31:3281–3287CrossRefGoogle Scholar
  15. Hagmar L, Björk J, Sjödin A, Bergman Å, Erfurth E-M (2001) Plasma levels of persistent organohalogens and hormone levels in adult male humans. Arch Environ Health 56:138–143PubMedGoogle Scholar
  16. Hallgren S, Darnerud PO (1998) Effects of polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs) and chlorinated paraffins (CPs) on thyroid hormone levels and enzyme activites in rats. Organohal Comp 35:391–394Google Scholar
  17. Hallgren S, Sinjari T, Håkansson H, Darnerud PO (2001) Effects of polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) on thyroid hormone and vitamin A levels in rats and mice. Arch Toxicol 75:200–208CrossRefPubMedGoogle Scholar
  18. Hooper K, McDonald TA (2000) The PBDEs: an emerging environmental challenge and another reason for breast-milk monitoring programs. Environ Health Perspect 108:387–392PubMedGoogle Scholar
  19. Hornung R, Reed L (1990) Estimation of average concentration in the presence of nondetectable values. Appl Occup Environ Hyg 5:46–51Google Scholar
  20. Hovander L, Malmberg T, Athanasiadou M, Athanassisdis I, Rahm S, Bergman Å, Klasson-Wehler E (2002) Identification of hydroylated PCB metabolites and other phenolic halogenated pollutants in human blood plasma. Arch Environ Contam Toxicol 42:105–117CrossRefPubMedGoogle Scholar
  21. Huwe J (2004) Polybrominated diphenyl ethers in meat samples collected from supermarkets across the US. BFR 2004. In: Proceedings of the third international workshop on brominated flame retardants, pp 41–44Google Scholar
  22. IPCS (1994) Brominated diphenyl ethers. World Health Organisation, GenevaGoogle Scholar
  23. Jansson B, Asplund L (1987) Brominated flame retardants—ubiquitous environmental pollutants. Chemosphere 16:2343–2349CrossRefGoogle Scholar
  24. Jansson B, Andersson R, Asplund L, Lizén K, Nylund K, Sellström U, Uvemo U, Wahlberg C, Widequist U, Odsjö T, Olsson M (1993) Chlorinated and brominated persistent organic compounds in biological samples from the environment. Environ Toxicol Chem 12:1163–1174Google Scholar
  25. Jaward F, Farrar N, Harner T, Sweetman A, Jonse K (2004a) Passive air sampling of PCBs, PBDEs, and organochlorine pesticides across Europe. Environ Sci Technol 38:34–41CrossRefGoogle Scholar
  26. Jaward F, Meijer S, Steinnes E, Thomas G, Jones K (2004b) Further studies on the latitudinal and temporal trends of persistent organic pollutants in Norwegian and UK background air. Environ Sci Technol 38:2523–2530CrossRefGoogle Scholar
  27. Karlsson M, Julander A, van Bavel B, Lindström G (2005) Solid-phase extraction of polybrominated diphenyl ethers in human plasma—comparison with an open column extraction method. Chromatographia 61:67–73CrossRefGoogle Scholar
  28. Law R, Allchin C, Bennett ME, Morris S, Rogan E (2002) Polybrominated diphenyl ethers in two species of marine top predators from England and Wales. Chemosphere 46:673–681CrossRefPubMedGoogle Scholar
  29. Lindström G, Wingfors H, Dam M, van Bavel B (1999) Identification of 19 polybrominated diphenyl ethers (PBDEs) in long-finned pilot whale (Globicephala melas) from the Atlantic. Arch Environ Cont Toxicol 36:355–363CrossRefGoogle Scholar
  30. Marsh G, Athanasiadou M, Bergman Å, Asplund L (2004) Identification of hydroxylated and methoxylated polybrominated diphenyl ethers in Baltic Sea salmon (Salmo salar) blood. Environ Sci Technol 38:10–18CrossRefPubMedGoogle Scholar
  31. Meerts IATM, Marsh G, van Leeuwen-Bol I, Luijks EAC, Jakobsson E, Bergman Å, Brouwer A (1998) Interaction of polybrominated diphenyl ether with metabolites (PBDE-OH) with human transthyretin in vitro. Organohal Comp 37:309–312Google Scholar
  32. Meironyté Guvenius D, Aronsson A, Ekman-Ordeberg G, Bergman Å, Norén K (2003) Human prenatal and postnatal exposure to polybrominated diphenyl ethers, polychlorinated biphenyls, polychlorinated biphenylols, and pentachlorophenol. Environ Health Perspect 111:1235–1241PubMedGoogle Scholar
  33. Nagayama J, Takasuga T, Tsuji H (2001) Contamination levels of brominated flame retardants, dioxins and organochlorine compounds in the blood of Japanese adults. In: Proceedings of the second international workshop on brominated flame retardants. Stockholm, Sweden, pp113–116Google Scholar
  34. Norén K, Meironyté D (2000) Certain organochlorine and organobromine contaminants in Swedish human milk in perspective of past 20–30 years. Chemosphere 40:1111–1123CrossRefPubMedGoogle Scholar
  35. Norris JM, Kociba RJ, Schwetz BA, Rose JQ, Humiston CG, Jewett GL, Gehring PJ, Mailhes JB (1975) Toxicology of octabromobiphenyl and decabromodiphenyl oxide. Environ Health Perspect 11:153–161PubMedGoogle Scholar
  36. Nylund K, Asplund L, Jansson B, Jonsson P, Litzén K, Sellström U (1992) Analysis of some polyhalogenated organic pollutants in sediment and sewage sludge. Chemosphere 24:1721–1730CrossRefGoogle Scholar
  37. Pettersson A, Westberg H, Engwall M, Ohlson C (2001) Concentrations in air and dust of polybrominated diphenyl ethers and tetrabromobisphenol A. In: Proceedings of the second international workshop on brominated flame retardants. Stockholm, Sweden, pp 223–226Google Scholar
  38. Pettersson-Julander A, van Bavel B, Engwall M, Westberg H (2004) Personal air sampling and analysis of polybrominated diphenyl ethers and other bromine containing compounds at an electronic recycling facility in Sweden. J Environ Monit 6:874–880CrossRefPubMedGoogle Scholar
  39. Schecter A, Päpke O, Tung K, Staskal D, Birnbaum L (2004) Polybrominated diphenyl ethers contamination of United States food. Environ Sci Technol 38:5306–5311CrossRefPubMedGoogle Scholar
  40. Sellström U (1999) Determination of some polybrominated flame retardants in biota, sediment, and sewage sludge. In: Proceedings of the environmental chemistry and institute of applied environmental research (ITM). Stockholm, Stockholm, p 71Google Scholar
  41. Sellström U, Kierkegaard A, Alsberg T, Jonsson P, Wahlberg C, de Wit C (1999) Brominated flame retardants in sediment from European estuaries, the Baltic Sea and in sewage sludge. Organohal Comp 40:383–386Google Scholar
  42. Sjödin A (2000) Occupational and dietary exposure to organohalogen substances, with special emphasis on polybrominated diphenyl ethers. In: Proceedings of the environmental chemistry. Stockholm, p 66Google Scholar
  43. Sjödin A, Jakobsson E, Kierkegaard A, Marsh G, Sellström U (1998) Gas chromatographic identification and quantification of polybrominated diphenyl ethers in a commercial product, Bromkal 70-5DE. J Chromatogr A 822:83–89CrossRefGoogle Scholar
  44. Sjödin A, Hagmar L, Klasson-Wehler E, Kronholm-Diab K, Jakobsson E, Bergman Å (1999) Flame retardant exposure: polybrominated diphenyl ethers in blood from Swedish workers. Environ Health Perspect 107:643–648PubMedGoogle Scholar
  45. Sjödin A, Carlsson H, Thuresson K, Sjölin S, Bergman Å, Östman C (2001) Flame retardants in indoor air at an electronics recycling plant and at other work environments. Environ Sci Technol 35:448–454CrossRefPubMedGoogle Scholar
  46. Stoker T, Laws S, Crofton KM, Hedge JM, Ferrell JM, Cooper RL (2004) Assessment of DE-71, a commercial polybrominated diphenyl ether (PBDE) mixture, in the EDSP male and female pubertal protocols. Toxicol Sci 78:144–155CrossRefPubMedGoogle Scholar
  47. Strandberg B, Dodder NG, Basu I, Hites R (2001) Concentrations and spatial variations of polybrominated diphenyl ethers and other organohalogen compounds in Great Lakes air. Environ Sci Technol 35:1078–1083CrossRefPubMedGoogle Scholar
  48. Thomsen C, Lundanes E, Becher G (2001) Brominated flame retardants in plasma samples from three different occupational groups in Norway. J Environ Monit 3:366–370CrossRefPubMedGoogle Scholar
  49. Thomsen C, Lundanes E, Becher G (2002) Brominated flame retardants in archived serum samples from norway: a study on temporal trends and the role of age. Environ Sci Technol 36:1414–1418CrossRefPubMedGoogle Scholar
  50. Thuresson K, Jakobsson L, Hagmar L, Sjödin A, Bergman Å (2002) Decabromodiphenyl ether exposure to workers manufacturing rubber and in an industrial setting producing rubber coated electric products. Organohal Comp 58:165–169Google Scholar
  51. Vetter W, Scholz E, Gaus C, Müller J, Haynes D (2001) Anthropogenic and natural organohalogen compounds in blubber of dolphins and dugongs (Dugond dugon) from Norhteast Australia. Arch Environ Contam Toxicol 41:221–231PubMedGoogle Scholar
  52. Vetter W, Stoll E, Garson M, Fahey S, Gaus C, Müller J (2002) Sponge halogenated natural products found at parts-per-million levels in marine mammals. Environ Toxicol Chem 21:2014–2019CrossRefPubMedGoogle Scholar
  53. Watanabe I, Kashimoto T, Tatsukawa R (1987) Polybrominated biphenyl ethers in marine fish, shellfish and river and marine sediments in Japan. Chemosphere 16:2389–2396CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • A. Julander
    • 1
  • M. Karlsson
    • 1
  • K. Hagström
    • 2
  • C. G. Ohlson
    • 2
  • M. Engwall
    • 1
  • I-L. Bryngelsson
    • 2
  • H. Westberg
    • 2
  • B. van Bavel
    • 1
  1. 1.Department of Natural Sciences, Man-Technology-Environment Research CentreÖrebro UniversityÖrebroSweden
  2. 2.Deprtment of Occupational and Environmental MedicineÖrebro University HospitalÖrebroSweden

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