Leukocyte 8-hydroxydeoxyguanosine and aromatic DNA adduct in coke-oven workers with polycyclic aromatic hydrocarbon exposure

  • J. Zhang
  • M. IchibaEmail author
  • T. Hanaoka
  • G. Pan
  • Y. Yamano
  • K. Hara
  • K. Takahashi
  • K. Tomokuni
Original Article



To investigate the potential for exposure to polycyclic aromatic hydrocarbons (PAHs) to induce oxidative DNA damage, we conducted a cross-sectional study in coke-oven workers employed at an iron–steel factory.


The study population contained 119 coke-oven workers from different work areas of the oven and 38 controls. Personal information on age, employment duration, smoking habit and alcohol consumption was obtained at an interview. Leukocyte 8-hydroxydeoxyguanosine (8-OHdG) was measured by high performance liquid chromatography with electrochemical detection. Leukocyte aromatic DNA adducts as effective dose, and urinary 1-hydroxypyren as internal dose, were also measured, and used to analyze the relationship of 8-OHdG with other biomarkers for PAH exposure, tobacco smoke and alcohol consumption.


The leukocyte 8-OHdG revealed a wide inter-individual variation. The highest 8-OHdG level was detected in bottom-workers of the coke-oven. There were significant differences among the four different work areas (P=0.02). We could not find significant correlation between 8-OHdG levels and urinary 1-hydroxypyrene, but a weakly positive correlation was found between 8-OHdG and leukocyte aromatic DNA adducts among all subjects (r=0.19 P=0.03). We could not observe any effect of smoking and alcohol drinking on 8-OHdG production.


We could not find clear evidence that PAH exposure induces oxidative DNA damage.


PAH Aromatic DNA adduct 8-Hydroxydeoxyguanosine 



We are grateful to D. Guan (Angang Public Health and Anti-Epidemic Station, China) and G. Gao (Angang Occupational Hygiene Institute, China) for their help with the epidemiological survey and sampling. This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Science, Sports, and Culture, Japan.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • J. Zhang
    • 1
    • 7
  • M. Ichiba
    • 1
    Email author
  • T. Hanaoka
    • 2
  • G. Pan
    • 3
  • Y. Yamano
    • 4
  • K. Hara
    • 5
  • K. Takahashi
    • 6
  • K. Tomokuni
    • 1
  1. 1.Department of Social and Environmental MedicineSaga Medical SchoolSagaJapan
  2. 2.Epidemiology and Biostatistics DivisionNational Cancer Research Institute EastKashiwaJapan
  3. 3.Liaoning Provincial Center for Disease Prevention and Control Heping DistrictShenyangChina
  4. 4.Department of Hygiene and Preventive MedicineShowa University School of MedicineTokyoJapan
  5. 5.Institute for Science of LaborKawasakiJapan
  6. 6.University of Occupational and Environmental HealthKitakyushuJapan
  7. 7.Institute of Microbiology and Epidemiology, Fengtai DistrictBeijingChina

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