Leukocyte 8-hydroxydeoxyguanosine and aromatic DNA adduct in coke-oven workers with polycyclic aromatic hydrocarbon exposure
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To investigate the potential for exposure to polycyclic aromatic hydrocarbons (PAHs) to induce oxidative DNA damage, we conducted a cross-sectional study in coke-oven workers employed at an iron–steel factory.
The study population contained 119 coke-oven workers from different work areas of the oven and 38 controls. Personal information on age, employment duration, smoking habit and alcohol consumption was obtained at an interview. Leukocyte 8-hydroxydeoxyguanosine (8-OHdG) was measured by high performance liquid chromatography with electrochemical detection. Leukocyte aromatic DNA adducts as effective dose, and urinary 1-hydroxypyren as internal dose, were also measured, and used to analyze the relationship of 8-OHdG with other biomarkers for PAH exposure, tobacco smoke and alcohol consumption.
The leukocyte 8-OHdG revealed a wide inter-individual variation. The highest 8-OHdG level was detected in bottom-workers of the coke-oven. There were significant differences among the four different work areas (P=0.02). We could not find significant correlation between 8-OHdG levels and urinary 1-hydroxypyrene, but a weakly positive correlation was found between 8-OHdG and leukocyte aromatic DNA adducts among all subjects (r=0.19 P=0.03). We could not observe any effect of smoking and alcohol drinking on 8-OHdG production.
We could not find clear evidence that PAH exposure induces oxidative DNA damage.
KeywordsPAH Aromatic DNA adduct 8-Hydroxydeoxyguanosine
We are grateful to D. Guan (Angang Public Health and Anti-Epidemic Station, China) and G. Gao (Angang Occupational Hygiene Institute, China) for their help with the epidemiological survey and sampling. This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Science, Sports, and Culture, Japan.
- Autrup H, Daneshvar B, Dragsted LO, Gamborg M, Hansen AM, Loft S, Okkels H, Nielsen F, Nielsen PS, Raffn E, Wallin H, Knudsen LE (1999) Biomarkers for exposure to ambient air pollution—comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress. Environ Health Perspect 107:233–238Google Scholar
- Carstensen U, Hou SM, Alexandrie AK, Hogstedt B, Tagesson C, Warholm M, Rannug A, Lambert B, Axmon A, Hagmar L (1999) Influence of genetic polymorphisms of biotransformation enzymes on gene mutations strand breaks of deoxyribonucleic acid and micronuclei in mononuclear blood cells and urinary 8-hydroxydeoxyguanosine in potroom workers exposed to polyaromatic hydrocarbons. Scand J Work Environ Health 25:351–360PubMedGoogle Scholar
- Farmer FB (1995) Monitoring of human exposure to carcinogens through DNA and protein adduct determination. Toxicol Lett 82–83:757–762Google Scholar
- Ichiba M, Wang Y, Oishi H, Iyadomi M, Shono N, Tomokuni K (1996) Smoking-related DNA adducts and genetic polymorphism for metabolic enzymes in human lymphocytes. Biomarkers 1:211–214Google Scholar
- International Agency for Research on Cancer (1984) Polynuclear aromatic compounds, part 3: Industrial exposures in aluminium production, coal gasification, coke production, and iron and steel founding. In: IARC monographs on the evaluation of the carcinogenic risk of chemicals in humans. Lyons, vol 34, pp 101–131Google Scholar
- Kasai H, Chung MH, Jones DS, Inoue H, Ishikawa H, Kamiya H, Ohtsuka E, Nishimura S (1991) 8-Hydroxyguanine, a DNA adduct formed by oxygen radicals: its implication on oxygen radical-involved mutagenesis/carcinogenesis. J Toxicol Sci 16 [Suppl 1]:95–105Google Scholar
- Marczynski B, Rihs HP, Rossbach B, Holzer J, Angerer J, Scherenberg M, Hoffmann G, Bruning T, Wilhelm M (2002) Analysis of 8-oxy-7,8-dihydro-2′-deoxyguanosine and DNA strand breaks in white blood cells of occupationally exposed workers: comparison with ambient monitoring, urinary metabolites and enzyme polymorphisms. Carcinogenesis 23:273–281CrossRefPubMedGoogle Scholar
- Tagesson C, Chabiuk D, Axelson O, Baranski B, Palus J, Wyszynska K (1993) Increased urinary excretion of the oxidative DNA adduct 8-hydroxydeoxyguanosine as a possible early indicator of occupational cancer hazards in the asbestos rubber and azo-dye industries. Pol J Occup Med Environ Health 6:357–368PubMedGoogle Scholar
- Takahashi K, Pan G, Kasai H, Hanaoka T, Feng Y, Liu N, Zhang S, Xu Z, Tsyda T, Yamato H, Higashi T, Okubo T (1997) Relationship between asbestos exposure and 8-hydroxydeoxyguanosine levels in leukocytic DNA of workers at a Chinese asbestos-material plant. Int J Occup Environ Health 3:111–119PubMedGoogle Scholar
- Yamano Y, Tokutake T, Haga M, Ishihara Y, Morinobu S, Kagawa J (1990) Determination of cotinine by high performance liquid chromatography and evaluation of exposure to tobacco smoke. Proceedings of 63rd Annual Meeting of Japan Association of Industrial Health (in Japanese), p126Google Scholar