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Histochemistry and Cell Biology

, Volume 109, Issue 1, pp 41–48 | Cite as

Loss of caveolin expression in type I pneumocytes as an indicator of subcellular alterations during lung fibrogenesis

  • M. Kasper
  • T. Reimann
  • U. Hempel
  • K.-W. Wenzel
  • A. Bierhaus
  • D. Schuh
  • V. Dimmer
  • G. Haroske
  • M. Müller
ORIGINAL PAPER

Abstract

 Caveolin is a major structural protein of caveolae, also known as plasmalemmal vesicles, which are particularly abundant in type I pneumocytes and capillary endothelial cells of lung parenchyma. Here we demonstrate that caveolin expression in the alveolar epithelium of rats and mini pigs is strikingly downregulated after irradiation-induced lung injury. Indirect immunoperoxidase staining with polyclonal anti-caveolin antibodies, confirmed by double fluorescence studies with type I cell-specific monoclonal anti-cytokeratin antibodies or lectins, revealed a dramatic loss of caveolin immunoreactivity in type I pneumocytes. In contrast, caveolin expression increased in endothelial cells. Immunoblotting of lung homogenates from normal and irradiated rats using specific anti-caveolin antibodies confirmed the presence of caveolin in normal tissue and its marked decrease of expression in fibrotic tissue. The loss of caveolin as an important structural protein of caveolae in alveolar epithelial cells may be an early indicator of serious type I cell injury during fibrogenesis. The increase of caveolin immunoreactivity in endothelia of blood vessels may indicate that different types of caveolae and/or different regulatory mechanisms of caveolin expression exist.

Keywords

Alveolar Epithelial Cell Capillary Endothelial Cell Alveolar Epithelium Fibrotic Tissue Lung Homogenate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • M. Kasper
    • 1
  • T. Reimann
    • 2
  • U. Hempel
    • 2
  • K.-W. Wenzel
    • 2
  • A. Bierhaus
    • 1
  • D. Schuh
    • 3
  • V. Dimmer
    • 3
  • G. Haroske
    • 3
  • M. Müller
    • 3
  1. 1.Institute of Anatomy, Technical University of Dresden, Fetscherstrasse 74, D-O1307 Dresden, Germany Tel. +49 351 458 2022; Fax: +49 351 458 5329DE
  2. 2.Institute of Physiological Chemistry, Technical University of Dresden, Karl Marx Allee 3, D-01109 Dresden
  3. 3.Institute of Pathology, Technical University of Dresden, Fetscherstrasse 74, D-01307 Dresden GermanyDE

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