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Histochemistry and Cell Biology

, Volume 115, Issue 1, pp 59–65 | Cite as

Green fluorescent protein (GFP) fusion constructs in gene therapy research

  • Jarmo Wahlfors
  • Sami Loimas
  • Tiina Pasanen
  • Tanja Hakkarainen
Review

Abstract

The history of green fluorescent protein (GFP) as a marker is less than 10 years old, but it has already made a major impact on many areas of natural sciences, especially on cell biology and histochemistry. GFP can be detected in living cells without selection or staining and it can be fused to other proteins to yield fluorescent chimeras. The potential of GFP has also been recognised by gene therapy researchers and various GFP-tagged therapeutic proteins have been constructed. These chimeric proteins have been used to determine the expression level, site and time course of the therapeutic gene, or the correlation between gene transfer rate and therapeutic outcome. This review summarises the status of the applications of GFP fusions in gene therapy research.

Gene therapy GFP fusion protein 

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Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • Jarmo Wahlfors
    • 1
  • Sami Loimas
    • 1
  • Tiina Pasanen
    • 1
  • Tanja Hakkarainen
    • 1
  1. 1.Gene Transfer Technology Group, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O. Box 1627, 70211 KuopioFinland

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