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Histochemistry and Cell Biology

, Volume 152, Issue 3, pp 207–215 | Cite as

Zw10 is a spindle assembly checkpoint protein that regulates meiotic maturation in mouse oocytes

  • Yuram Park
  • Jae-Sung KimEmail author
  • Jeong Su OhEmail author
Original Paper

Abstract

Faithful chromosome segregation during the cell cycle is ensured by the spindle assembly checkpoint (SAC). Although SAC activity is highly conserved and most organisms share common SAC components, additional proteins that regulate SAC activity to ensure high fidelity chromosome segregation are present in higher eukaryotes. Zw10 is one of these additional SAC components. Although Zw10 has been demonstrated to be involved in SAC activity during mitosis, little is known about its role during oocyte meiosis. Here, we report that Zw10 is localized at the kinetochore and is required for SAC activation during meiotic maturation. Knockdown of Zw10 led to precocious polar body extrusion by impairing Mad2 recruitment at kinetochores. Moreover, Zw10 knockdown impaired chromosome alignment and kinetochore–microtubule attachment, increasing the incidence of aneuploidy. Furthermore, Zw10 expression decreased with maternal age, suggesting that Zw10 is associated with the age-related increase in the incidence of aneuploidy. Together, our results demonstrate that Zw10 is localized at kinetochores and functions as an essential SAC component in mouse oocytes.

Keywords

Oocyte Spindle assembly checkpoint Zw10 Kinetochore Chromosome segregation 

Notes

Acknowledgements

We thank Hyuk-Joon Jeon for contributing a preliminary experiment. This work was supported by grants from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2016R1A2B4006752) and Ministry of Education (NRF-2017R1A6A1A03015642).

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Integrative Biotechnology, College of Biotechnology and BioengineeringSungkyunkwan UniversitySuwonKorea
  2. 2.Division of Radiation Cancer ResearchKorea Institute of Radiological and Medical SciencesSeoulRepublic of Korea

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